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Molecular characterization of the complete genome of human influenza H5N1 virus isolates from Thailand.
J Gen Virol. 2005 Feb; 86(Pt 2):423-33.JG

Abstract

The complete genomes of three human H5N1 influenza isolates were characterized, together with the haemagglutinin (HA) and neuraminidase (NA) genes from two additional human isolates and one chicken isolate. These six influenza isolates were obtained from four different provinces of Thailand during the avian influenza outbreak in Asia from late 2003 to May 2004. All six Thailand isolates contained multiple basic amino acids at the cleavage site in the HA gene. Amino acid residues at the receptor-binding site of the five human viruses were similar to those of the chicken virus and other H5N1 viruses from Hong Kong. The presence of amantadine resistance in the Thailand viruses isolated during this outbreak was suggested by a fixed mutation in M2 and confirmed by a phenotypic assay. All genomic segments of the Thailand viruses clustered with the recently described genotype Z. The Thailand viruses contained more avian-specific residues than the 1997 Hong Kong H5N1 viruses, suggesting that the virus may have adapted to allow a more efficient spread in avian species.

Authors+Show Affiliations

Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. siput@mahidol.ac.thNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15659762

Citation

Puthavathana, Pilaipan, et al. "Molecular Characterization of the Complete Genome of Human Influenza H5N1 Virus Isolates From Thailand." The Journal of General Virology, vol. 86, no. Pt 2, 2005, pp. 423-33.
Puthavathana P, Auewarakul P, Charoenying PC, et al. Molecular characterization of the complete genome of human influenza H5N1 virus isolates from Thailand. J Gen Virol. 2005;86(Pt 2):423-33.
Puthavathana, P., Auewarakul, P., Charoenying, P. C., Sangsiriwut, K., Pooruk, P., Boonnak, K., Khanyok, R., Thawachsupa, P., Kijphati, R., & Sawanpanyalert, P. (2005). Molecular characterization of the complete genome of human influenza H5N1 virus isolates from Thailand. The Journal of General Virology, 86(Pt 2), 423-33.
Puthavathana P, et al. Molecular Characterization of the Complete Genome of Human Influenza H5N1 Virus Isolates From Thailand. J Gen Virol. 2005;86(Pt 2):423-33. PubMed PMID: 15659762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of the complete genome of human influenza H5N1 virus isolates from Thailand. AU - Puthavathana,Pilaipan, AU - Auewarakul,Prasert, AU - Charoenying,Pakapak Chor, AU - Sangsiriwut,Kantima, AU - Pooruk,Phisanu, AU - Boonnak,Kobporn, AU - Khanyok,Raweewan, AU - Thawachsupa,Pranee, AU - Kijphati,Rungrueng, AU - Sawanpanyalert,Pathom, PY - 2005/1/22/pubmed PY - 2005/3/10/medline PY - 2005/1/22/entrez SP - 423 EP - 33 JF - The Journal of general virology JO - J. Gen. Virol. VL - 86 IS - Pt 2 N2 - The complete genomes of three human H5N1 influenza isolates were characterized, together with the haemagglutinin (HA) and neuraminidase (NA) genes from two additional human isolates and one chicken isolate. These six influenza isolates were obtained from four different provinces of Thailand during the avian influenza outbreak in Asia from late 2003 to May 2004. All six Thailand isolates contained multiple basic amino acids at the cleavage site in the HA gene. Amino acid residues at the receptor-binding site of the five human viruses were similar to those of the chicken virus and other H5N1 viruses from Hong Kong. The presence of amantadine resistance in the Thailand viruses isolated during this outbreak was suggested by a fixed mutation in M2 and confirmed by a phenotypic assay. All genomic segments of the Thailand viruses clustered with the recently described genotype Z. The Thailand viruses contained more avian-specific residues than the 1997 Hong Kong H5N1 viruses, suggesting that the virus may have adapted to allow a more efficient spread in avian species. SN - 0022-1317 UR - https://www.unboundmedicine.com/medline/citation/15659762/Molecular_characterization_of_the_complete_genome_of_human_influenza_H5N1_virus_isolates_from_Thailand_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/vir.0.80368-0 DB - PRIME DP - Unbound Medicine ER -