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Activity deprivation-dependent induction of the proapoptotic BH3-only protein Bim is independent of JNK/c-Jun activation during apoptosis in cerebellar granule neurons.
Neurosci Lett. 2005 Feb 25; 375(1):7-12.NL

Abstract

Bcl-2-interacting mediator of cell death (Bim), a proapoptotic BH3-only protein, plays a critical role in neuronal apoptosis. Cerebellar granule neurons (CGNs) depend on activity for their survival and undergo apoptosis when deprived of depolarizing concentration of KCl. While it has been proposed that the activation of c-Jun NH2-terminal protein kinase (JNK)/c-Jun pathway contributes to the upregulation of bim gene in neurons subjected to survival signaling withdrawal, here we show that neither inhibition of JNK activity nor expression of dominant-negative c-Jun suppresses the expression of bim gene induced by activity deprivation in CGNs. We conclude that induction of bim gene is independent of the activation of JNK/c-Jun signaling pathway by activity deprivation during apoptosis of CGNs.

Authors+Show Affiliations

Shi L
Department of Pharmacology, Zhongshan Medical College, SUN Yat-sen University, No. 74, Zhongshan Road 2, Guangzhou 510080, China.
Gong S
No affiliation info available
Yuan Z
No affiliation info available
Ma C
No affiliation info available
Liu Y
No affiliation info available
Wang C
No affiliation info available
Li W
No affiliation info available
Pi R
No affiliation info available
Huang S
No affiliation info available
Chen R
No affiliation info available
Han Y
No affiliation info available
Mao Z
No affiliation info available
Li M
No affiliation info available

MeSH

AnimalsAnimals, NewbornApoptosisApoptosis Regulatory ProteinsBlotting, WesternCells, CulturedCerebellumDose-Response Relationship, DrugDrug InteractionsEnzyme ActivationEnzyme InhibitorsGene Expression RegulationGreen Fluorescent ProteinsJNK Mitogen-Activated Protein KinasesNeural InhibitionNeuronsNeuropeptidesPhosphorylationPotassium ChlorideProto-Oncogene Proteins c-junRNA, MessengerRatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionSerineSignal TransductionTime FactorsTransfection

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15664113

Citation

Shi, Leyu, et al. "Activity Deprivation-dependent Induction of the Proapoptotic BH3-only Protein Bim Is Independent of JNK/c-Jun Activation During Apoptosis in Cerebellar Granule Neurons." Neuroscience Letters, vol. 375, no. 1, 2005, pp. 7-12.
Shi L, Gong S, Yuan Z, et al. Activity deprivation-dependent induction of the proapoptotic BH3-only protein Bim is independent of JNK/c-Jun activation during apoptosis in cerebellar granule neurons. Neurosci Lett. 2005;375(1):7-12.
Shi, L., Gong, S., Yuan, Z., Ma, C., Liu, Y., Wang, C., Li, W., Pi, R., Huang, S., Chen, R., Han, Y., Mao, Z., & Li, M. (2005). Activity deprivation-dependent induction of the proapoptotic BH3-only protein Bim is independent of JNK/c-Jun activation during apoptosis in cerebellar granule neurons. Neuroscience Letters, 375(1), 7-12.
Shi L, et al. Activity Deprivation-dependent Induction of the Proapoptotic BH3-only Protein Bim Is Independent of JNK/c-Jun Activation During Apoptosis in Cerebellar Granule Neurons. Neurosci Lett. 2005 Feb 25;375(1):7-12. PubMed PMID: 15664113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activity deprivation-dependent induction of the proapoptotic BH3-only protein Bim is independent of JNK/c-Jun activation during apoptosis in cerebellar granule neurons. AU - Shi,Leyu, AU - Gong,Shoufang, AU - Yuan,Zhongmin, AU - Ma,Chi, AU - Liu,Yanling, AU - Wang,Chuanfu, AU - Li,Wenming, AU - Pi,Rongbiao, AU - Huang,Shoujian, AU - Chen,Ruzhu, AU - Han,Yifan, AU - Mao,Zixu, AU - Li,Mingtao, Y1 - 2004/11/24/ PY - 2004/08/25/received PY - 2004/10/02/revised PY - 2004/10/22/accepted PY - 2005/1/25/pubmed PY - 2005/4/19/medline PY - 2005/1/25/entrez SP - 7 EP - 12 JF - Neuroscience letters JO - Neurosci Lett VL - 375 IS - 1 N2 - Bcl-2-interacting mediator of cell death (Bim), a proapoptotic BH3-only protein, plays a critical role in neuronal apoptosis. Cerebellar granule neurons (CGNs) depend on activity for their survival and undergo apoptosis when deprived of depolarizing concentration of KCl. While it has been proposed that the activation of c-Jun NH2-terminal protein kinase (JNK)/c-Jun pathway contributes to the upregulation of bim gene in neurons subjected to survival signaling withdrawal, here we show that neither inhibition of JNK activity nor expression of dominant-negative c-Jun suppresses the expression of bim gene induced by activity deprivation in CGNs. We conclude that induction of bim gene is independent of the activation of JNK/c-Jun signaling pathway by activity deprivation during apoptosis of CGNs. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/15664113/Activity_deprivation_dependent_induction_of_the_proapoptotic_BH3_only_protein_Bim_is_independent_of_JNK/c_Jun_activation_during_apoptosis_in_cerebellar_granule_neurons_ DB - PRIME DP - Unbound Medicine ER -