Tags

Type your tag names separated by a space and hit enter

Construction of minimum size cellulase (Cel5Z) from Pectobacterium chrysanthemi PY35 by removal of the C-terminal region.
Appl Microbiol Biotechnol. 2005 Jul; 68(1):46-52.AM

Abstract

Pectobacterium chrysanthemi PY35 secretes the endoglucanase Cel5Z, an enzyme of the glycoside hydrolase family 5. Cel5Z is a 426 amino acid, signal peptide (SP)-containing protein composed of two domains: a large N-terminal catalytic domain (CD; 291 amino acids) and a small C-terminal cellulose binding domain (CBD; 62 amino acids). These two domains are separated by a 30 amino acid linker region (LR). A truncated cel5Z gene was constructed with the addition of a nonsense mutation that removes the C-terminal region of the protein. A truncated Cel5Z protein, consisting of 280 amino acid residues, functioned as a mature enzyme despite the absence of the SP, 11 amino acid CD, LR, and CBD region. In fact, this truncated Cel5Z protein showed an enzymatic activity 80% higher than that of full-length Cel5Z. However, cellulase activity was undetectable in mature Cel5Z proteins truncated to less than 280 amino acids.

Authors+Show Affiliations

Division of Applied Life Science, Gyeongsang National University, Chinju, 660-701, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15666145

Citation

Lim, Woo Jin, et al. "Construction of Minimum Size Cellulase (Cel5Z) From Pectobacterium Chrysanthemi PY35 By Removal of the C-terminal Region." Applied Microbiology and Biotechnology, vol. 68, no. 1, 2005, pp. 46-52.
Lim WJ, Hong SY, An CL, et al. Construction of minimum size cellulase (Cel5Z) from Pectobacterium chrysanthemi PY35 by removal of the C-terminal region. Appl Microbiol Biotechnol. 2005;68(1):46-52.
Lim, W. J., Hong, S. Y., An, C. L., Cho, K. M., Choi, B. R., Kim, Y. K., An, J. M., Kang, J. M., Lee, S. M., Cho, S. J., Kim, H., & Yun, H. D. (2005). Construction of minimum size cellulase (Cel5Z) from Pectobacterium chrysanthemi PY35 by removal of the C-terminal region. Applied Microbiology and Biotechnology, 68(1), 46-52.
Lim WJ, et al. Construction of Minimum Size Cellulase (Cel5Z) From Pectobacterium Chrysanthemi PY35 By Removal of the C-terminal Region. Appl Microbiol Biotechnol. 2005;68(1):46-52. PubMed PMID: 15666145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of minimum size cellulase (Cel5Z) from Pectobacterium chrysanthemi PY35 by removal of the C-terminal region. AU - Lim,Woo Jin, AU - Hong,Su Young, AU - An,Chang Long, AU - Cho,Kye Man, AU - Choi,Byoung Rock, AU - Kim,Young Kyun, AU - An,Jin Mee, AU - Kang,Jung Mi, AU - Lee,Sun Mi, AU - Cho,Soo Jeong, AU - Kim,Hoon, AU - Yun,Han Dae, Y1 - 2005/01/22/ PY - 2004/11/10/received PY - 2004/12/13/accepted PY - 2004/12/06/revised PY - 2005/1/25/pubmed PY - 2005/11/3/medline PY - 2005/1/25/entrez SP - 46 EP - 52 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 68 IS - 1 N2 - Pectobacterium chrysanthemi PY35 secretes the endoglucanase Cel5Z, an enzyme of the glycoside hydrolase family 5. Cel5Z is a 426 amino acid, signal peptide (SP)-containing protein composed of two domains: a large N-terminal catalytic domain (CD; 291 amino acids) and a small C-terminal cellulose binding domain (CBD; 62 amino acids). These two domains are separated by a 30 amino acid linker region (LR). A truncated cel5Z gene was constructed with the addition of a nonsense mutation that removes the C-terminal region of the protein. A truncated Cel5Z protein, consisting of 280 amino acid residues, functioned as a mature enzyme despite the absence of the SP, 11 amino acid CD, LR, and CBD region. In fact, this truncated Cel5Z protein showed an enzymatic activity 80% higher than that of full-length Cel5Z. However, cellulase activity was undetectable in mature Cel5Z proteins truncated to less than 280 amino acids. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/15666145/Construction_of_minimum_size_cellulase__Cel5Z__from_Pectobacterium_chrysanthemi_PY35_by_removal_of_the_C_terminal_region_ L2 - https://dx.doi.org/10.1007/s00253-004-1880-3 DB - PRIME DP - Unbound Medicine ER -