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Virulence genes and intimin types of Shiga-toxin-producing Escherichia coli isolated from cattle and beef products in Argentina.
Int Microbiol. 2004 Dec; 7(4):269-76.IM

Abstract

A total of 153 Shiga-toxin-producing Escherichia coli (STEC) isolates from feces of cattle and beef products (hamburgers and ground beef) in Argentina were characterized in this study. PCR showed that 22 (14%) isolates carried stx1 genes, 113 (74%) possessed stx2 genes and 18 (12%) both stx1 and stx2. Intimin (eae), enterohemolysin (ehxA), and STEC autoagglutinating adhesin (saa) virulence genes were detected in 36 (24%), 70 (46%) and in 34 (22%) of the isolates, respectively. None of 34 saa-positive isolates carried the gene eae, and 31 were ehxA-positive. Fourteen (7 of serotype O26:H11 and 4 of serotype O5:H-) isolates had intimin b1, 16 isolates possessed intimin g1 (11 of serotype O145:H- and 5 of serotype O157:H7), 5 isolates had intimin type e1 (4 of serotypes O103:H- and O103:H2), and one isolate O111:H- showed intimin type q/g2. Although the 153 STEC isolates belonged to 63 different seropathotypes, only 12 accounted for 58% of isolates. Seropathotype ONT:H- stx2 (18 isolates) was the most common, followed by O171:H2 stx2 (12 isolates), etc. The majority (84%) of STEC isolates belonged to serotypes previously found in human STEC and 56% to serotypes associated with STEC isolated from patients with hemolytic uremic syndrome (HUS). Thus, this study confirms that cattle are a major reservoir of STEC pathogenic for humans. To our knowledge, this is the first study that described the presence of saa gene in STEC of serotypes O20:H19, O39:H49, O74:H28, O79:H19, O116:H21, O120:H19, O141:H7, O141:H8, O174:H21, and ONT:H21. The serotypes O120:H19 and O185:H7 were not previously reported in bovine STEC.

Authors+Show Affiliations

E. coli Reference Laboratory (LREC), Department of Microbiology and Parasitology, Faculty of Veterinary, University of Santiago de Compostela, Lugo, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15666247

Citation

Blanco, Miguel, et al. "Virulence Genes and Intimin Types of Shiga-toxin-producing Escherichia Coli Isolated From Cattle and Beef Products in Argentina." International Microbiology : the Official Journal of the Spanish Society for Microbiology, vol. 7, no. 4, 2004, pp. 269-76.
Blanco M, Padola NL, Krüger A, et al. Virulence genes and intimin types of Shiga-toxin-producing Escherichia coli isolated from cattle and beef products in Argentina. Int Microbiol. 2004;7(4):269-76.
Blanco, M., Padola, N. L., Krüger, A., Sanz, M. E., Blanco, J. E., González, E. A., Dahbi, G., Mora, A., Bernárdez, M. I., Etcheverría, A. I., Arroyo, G. H., Lucchesi, P. M., Parma, A. E., & Blanco, J. (2004). Virulence genes and intimin types of Shiga-toxin-producing Escherichia coli isolated from cattle and beef products in Argentina. International Microbiology : the Official Journal of the Spanish Society for Microbiology, 7(4), 269-76.
Blanco M, et al. Virulence Genes and Intimin Types of Shiga-toxin-producing Escherichia Coli Isolated From Cattle and Beef Products in Argentina. Int Microbiol. 2004;7(4):269-76. PubMed PMID: 15666247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Virulence genes and intimin types of Shiga-toxin-producing Escherichia coli isolated from cattle and beef products in Argentina. AU - Blanco,Miguel, AU - Padola,Nora L, AU - Krüger,Alejandra, AU - Sanz,Marcelo E, AU - Blanco,Jesús E, AU - González,Enrique A, AU - Dahbi,Ghizlane, AU - Mora,Azucena, AU - Bernárdez,María Isabel, AU - Etcheverría,Analía I, AU - Arroyo,Guillermo H, AU - Lucchesi,Paula M A, AU - Parma,Alberto E, AU - Blanco,Jorge, PY - 2005/1/25/pubmed PY - 2005/4/6/medline PY - 2005/1/25/entrez SP - 269 EP - 76 JF - International microbiology : the official journal of the Spanish Society for Microbiology JO - Int Microbiol VL - 7 IS - 4 N2 - A total of 153 Shiga-toxin-producing Escherichia coli (STEC) isolates from feces of cattle and beef products (hamburgers and ground beef) in Argentina were characterized in this study. PCR showed that 22 (14%) isolates carried stx1 genes, 113 (74%) possessed stx2 genes and 18 (12%) both stx1 and stx2. Intimin (eae), enterohemolysin (ehxA), and STEC autoagglutinating adhesin (saa) virulence genes were detected in 36 (24%), 70 (46%) and in 34 (22%) of the isolates, respectively. None of 34 saa-positive isolates carried the gene eae, and 31 were ehxA-positive. Fourteen (7 of serotype O26:H11 and 4 of serotype O5:H-) isolates had intimin b1, 16 isolates possessed intimin g1 (11 of serotype O145:H- and 5 of serotype O157:H7), 5 isolates had intimin type e1 (4 of serotypes O103:H- and O103:H2), and one isolate O111:H- showed intimin type q/g2. Although the 153 STEC isolates belonged to 63 different seropathotypes, only 12 accounted for 58% of isolates. Seropathotype ONT:H- stx2 (18 isolates) was the most common, followed by O171:H2 stx2 (12 isolates), etc. The majority (84%) of STEC isolates belonged to serotypes previously found in human STEC and 56% to serotypes associated with STEC isolated from patients with hemolytic uremic syndrome (HUS). Thus, this study confirms that cattle are a major reservoir of STEC pathogenic for humans. To our knowledge, this is the first study that described the presence of saa gene in STEC of serotypes O20:H19, O39:H49, O74:H28, O79:H19, O116:H21, O120:H19, O141:H7, O141:H8, O174:H21, and ONT:H21. The serotypes O120:H19 and O185:H7 were not previously reported in bovine STEC. SN - 1139-6709 UR - https://www.unboundmedicine.com/medline/citation/15666247/Virulence_genes_and_intimin_types_of_Shiga_toxin_producing_Escherichia_coli_isolated_from_cattle_and_beef_products_in_Argentina_ L2 - http://www.im.microbios.org/0704/0704269.pdf DB - PRIME DP - Unbound Medicine ER -