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Tissue tropism and bursal transformation ability of subgroup J avian leukosis virus in White Leghorn chickens.
Avian Dis. 2004 Dec; 48(4):921-7.AD

Abstract

In Experiment 1, a monoclonal antibody against the envelope glycoprotein (gp85) of subgroup J avian leukosis virus (ALV-J) was used to study the distribution of ALV-J in various tissues of White Leghorn chickens inoculated as embryos with the strain ADOL-Hcl of ALV-J. At 2 and 6 wk of age, various tissues from infected and control uninfected chickens were tested for the presence of ALV-J gp85 by immunohistochemistry. In Experiment 2, using the methyl green-pyronine (MGP) stain, sections of bursa of Fabricius (BF) from chickens of line 15I5 x 7(1), inoculated with ALV-J or Rous-associated virus-1 (RAV-1), a subgroup A ALV, at hatch were examined for transformation of bursal follicles at 4 and 10 wk of age. In Experiment 1, specific staining indicative of the presence of ALV-J gp85 was noted at both 2 and 6 wk of age in the adrenal gland, bursa, gonads, heart, kidney, liver, bone marrow, nerve, pancreas, proventriculus, spleen, and thymus. In Experiment 2, by 10 wk of age, transformed bursal follicles were detected in MGP-stained sections of BF in only one of five (20%) chickens inoculated with ALV-J at hatch, compared with five of five (100%) chickens inoculated with RAV-1. The data demonstrate distribution of ALV-J gp85 in various tissues of White Leghorn chickens experimentally inoculated as embryos with the virus. The data also confirm our previous observation that ALV-J is capable of inducing transformation of bursal follicles, albeit the incidence is less frequent than that induced by subgroup A ALV.

Authors+Show Affiliations

USDA-Agricultural Research Service, Avian Disease and Oncology Laboratory, 3606 East Mount Hope Road, East Lansing, MI 48823, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15666876

Citation

Williams, Susan M., et al. "Tissue Tropism and Bursal Transformation Ability of Subgroup J Avian Leukosis Virus in White Leghorn Chickens." Avian Diseases, vol. 48, no. 4, 2004, pp. 921-7.
Williams SM, Fitzgerald SD, Reed WM, et al. Tissue tropism and bursal transformation ability of subgroup J avian leukosis virus in White Leghorn chickens. Avian Dis. 2004;48(4):921-7.
Williams, S. M., Fitzgerald, S. D., Reed, W. M., Lee, L. F., & Fadly, A. M. (2004). Tissue tropism and bursal transformation ability of subgroup J avian leukosis virus in White Leghorn chickens. Avian Diseases, 48(4), 921-7.
Williams SM, et al. Tissue Tropism and Bursal Transformation Ability of Subgroup J Avian Leukosis Virus in White Leghorn Chickens. Avian Dis. 2004;48(4):921-7. PubMed PMID: 15666876.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue tropism and bursal transformation ability of subgroup J avian leukosis virus in White Leghorn chickens. AU - Williams,Susan M, AU - Fitzgerald,Scott D, AU - Reed,Willie M, AU - Lee,Lucy F, AU - Fadly,Aly M, PY - 2005/1/26/pubmed PY - 2005/3/16/medline PY - 2005/1/26/entrez SP - 921 EP - 7 JF - Avian diseases JO - Avian Dis VL - 48 IS - 4 N2 - In Experiment 1, a monoclonal antibody against the envelope glycoprotein (gp85) of subgroup J avian leukosis virus (ALV-J) was used to study the distribution of ALV-J in various tissues of White Leghorn chickens inoculated as embryos with the strain ADOL-Hcl of ALV-J. At 2 and 6 wk of age, various tissues from infected and control uninfected chickens were tested for the presence of ALV-J gp85 by immunohistochemistry. In Experiment 2, using the methyl green-pyronine (MGP) stain, sections of bursa of Fabricius (BF) from chickens of line 15I5 x 7(1), inoculated with ALV-J or Rous-associated virus-1 (RAV-1), a subgroup A ALV, at hatch were examined for transformation of bursal follicles at 4 and 10 wk of age. In Experiment 1, specific staining indicative of the presence of ALV-J gp85 was noted at both 2 and 6 wk of age in the adrenal gland, bursa, gonads, heart, kidney, liver, bone marrow, nerve, pancreas, proventriculus, spleen, and thymus. In Experiment 2, by 10 wk of age, transformed bursal follicles were detected in MGP-stained sections of BF in only one of five (20%) chickens inoculated with ALV-J at hatch, compared with five of five (100%) chickens inoculated with RAV-1. The data demonstrate distribution of ALV-J gp85 in various tissues of White Leghorn chickens experimentally inoculated as embryos with the virus. The data also confirm our previous observation that ALV-J is capable of inducing transformation of bursal follicles, albeit the incidence is less frequent than that induced by subgroup A ALV. SN - 0005-2086 UR - https://www.unboundmedicine.com/medline/citation/15666876/Tissue_tropism_and_bursal_transformation_ability_of_subgroup_J_avian_leukosis_virus_in_White_Leghorn_chickens_ L2 - https://doi.org/10.1637/7196-041904R DB - PRIME DP - Unbound Medicine ER -