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Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR.

Abstract

OBJECTIVE

The gut microbiota may contribute to the onset and maintenance of irritable bowel syndrome (IBS). In this study, the microbiotas of patients suffering from IBS were compared with a control group devoid of gastrointestinal (GI) symptoms.

METHODS

Fecal microbiota of patients (n = 27) fulfilling the Rome II criteria for IBS was compared with age- and gender-matched control subjects (n = 22). Fecal samples were obtained at 3 months intervals. Total bacterial DNA was analyzed by 20 quantitative real-time PCR assays covering approximately 300 bacterial species.

RESULTS

Extensive individual variation was observed in the GI microbiota among both the IBS- and control groups. Sorting of the IBS patients according to the symptom subtypes (diarrhea, constipation, and alternating predominant type) revealed that lower amounts of Lactobacillus spp. were present in the samples of diarrhea predominant IBS patients whereas constipation predominant IBS patients carried increased amounts of Veillonella spp. Average results from three fecal samples suggested differences in the Clostridium coccoides subgroup and Bifidobacterium catenulatum group between IBS patients (n = 21) and controls (n = 15). Of the intestinal pathogens earlier associated with IBS, no indications of Helicobacter spp. or Clostridium difficile were found whereas one case of Campylobacter jejuni was identified by sequencing.

CONCLUSIONS

With these real-time PCR assays, quantitative alterations in the GI microbiota of IBS patients were found. Increasing microbial DNA sequence information will further allow designing of new real-time PCR assays for a more extensive analysis of intestinal microbes in IBS.

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  • Authors+Show Affiliations

    ,

    Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Section of Microbiology, P.O. Box 66, FIN-00014 University of Helsinki, Finland.

    , , , , , , ,

    Source

    MeSH

    Adult
    Aged
    Bacteria
    DNA, Bacterial
    Feces
    Female
    Humans
    Irritable Bowel Syndrome
    Male
    Middle Aged
    Polymerase Chain Reaction
    Sequence Analysis, DNA

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    15667495

    Citation

    Malinen, Erja, et al. "Analysis of the Fecal Microbiota of Irritable Bowel Syndrome Patients and Healthy Controls With Real-time PCR." The American Journal of Gastroenterology, vol. 100, no. 2, 2005, pp. 373-82.
    Malinen E, Rinttilä T, Kajander K, et al. Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR. Am J Gastroenterol. 2005;100(2):373-82.
    Malinen, E., Rinttilä, T., Kajander, K., Mättö, J., Kassinen, A., Krogius, L., ... Palva, A. (2005). Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR. The American Journal of Gastroenterology, 100(2), pp. 373-82.
    Malinen E, et al. Analysis of the Fecal Microbiota of Irritable Bowel Syndrome Patients and Healthy Controls With Real-time PCR. Am J Gastroenterol. 2005;100(2):373-82. PubMed PMID: 15667495.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR. AU - Malinen,Erja, AU - Rinttilä,Teemu, AU - Kajander,Kajsa, AU - Mättö,Jaana, AU - Kassinen,Anna, AU - Krogius,Lotta, AU - Saarela,Maria, AU - Korpela,Riitta, AU - Palva,Airi, PY - 2005/1/26/pubmed PY - 2005/3/2/medline PY - 2005/1/26/entrez SP - 373 EP - 82 JF - The American journal of gastroenterology JO - Am. J. Gastroenterol. VL - 100 IS - 2 N2 - OBJECTIVE: The gut microbiota may contribute to the onset and maintenance of irritable bowel syndrome (IBS). In this study, the microbiotas of patients suffering from IBS were compared with a control group devoid of gastrointestinal (GI) symptoms. METHODS: Fecal microbiota of patients (n = 27) fulfilling the Rome II criteria for IBS was compared with age- and gender-matched control subjects (n = 22). Fecal samples were obtained at 3 months intervals. Total bacterial DNA was analyzed by 20 quantitative real-time PCR assays covering approximately 300 bacterial species. RESULTS: Extensive individual variation was observed in the GI microbiota among both the IBS- and control groups. Sorting of the IBS patients according to the symptom subtypes (diarrhea, constipation, and alternating predominant type) revealed that lower amounts of Lactobacillus spp. were present in the samples of diarrhea predominant IBS patients whereas constipation predominant IBS patients carried increased amounts of Veillonella spp. Average results from three fecal samples suggested differences in the Clostridium coccoides subgroup and Bifidobacterium catenulatum group between IBS patients (n = 21) and controls (n = 15). Of the intestinal pathogens earlier associated with IBS, no indications of Helicobacter spp. or Clostridium difficile were found whereas one case of Campylobacter jejuni was identified by sequencing. CONCLUSIONS: With these real-time PCR assays, quantitative alterations in the GI microbiota of IBS patients were found. Increasing microbial DNA sequence information will further allow designing of new real-time PCR assays for a more extensive analysis of intestinal microbes in IBS. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/15667495/Analysis_of_the_fecal_microbiota_of_irritable_bowel_syndrome_patients_and_healthy_controls_with_real_time_PCR_ L2 - http://Insights.ovid.com/pubmed?pmid=15667495 DB - PRIME DP - Unbound Medicine ER -