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Ultrastructural localization of matrix metalloproteinase-9 in eosinophils from the cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis.
Ann Trop Med Parasitol. 2004 Dec; 98(8):831-41.AT

Abstract

Matrix metalloproteinase-9 (MMP-9) has been implicated in the pathogenesis of eosinophilic meningitis caused by Angiostrongylus cantonensis. In the present study, such meningitis in mice was found to be associated with elevated expression of MMP-9 mRNA, elevated MMP-9 concentrations and enhanced MMP-9 activity in the cerebrospinal fluid (CSF). Immunocytochemistry showed that an anti-MMP-9 antibody reacted with macrophages, neutrophils and eosinophils from the CSF. As eosinophils are generally considered to be effector cells in host defence against A. cantonensis infection, high-resolution immuno-electron microscopy was then used to confirm the localization of MMP-9 in the eosinophils from the CSF. The method used, which was based on immunogold, indicated that the eosinophilic MMP-9 was mostly localized in the 'small' granules in the cytoplasm and along the cell membrane, and not in the crystalloid-containing secretory granules observed. It therefore appears that MMP-9 is synthesised and/or stored in the small granules of the eosinophils, and is released into the subarachnoid space of the host's brain by secretion or cell rupture.

Authors+Show Affiliations

Department of Entomology, National Chung-Hsing University, 250, Kuo-Kwang Road, Taichung 402, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15667715

Citation

Tseng, Y K., et al. "Ultrastructural Localization of Matrix Metalloproteinase-9 in Eosinophils From the Cerebrospinal Fluid of Mice With Eosinophilic Meningitis Caused By Angiostrongylus Cantonensis." Annals of Tropical Medicine and Parasitology, vol. 98, no. 8, 2004, pp. 831-41.
Tseng YK, Tu WC, Lee HH, et al. Ultrastructural localization of matrix metalloproteinase-9 in eosinophils from the cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis. Ann Trop Med Parasitol. 2004;98(8):831-41.
Tseng, Y. K., Tu, W. C., Lee, H. H., Chen, K. M., Chou, H. L., & Lai, S. C. (2004). Ultrastructural localization of matrix metalloproteinase-9 in eosinophils from the cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis. Annals of Tropical Medicine and Parasitology, 98(8), 831-41.
Tseng YK, et al. Ultrastructural Localization of Matrix Metalloproteinase-9 in Eosinophils From the Cerebrospinal Fluid of Mice With Eosinophilic Meningitis Caused By Angiostrongylus Cantonensis. Ann Trop Med Parasitol. 2004;98(8):831-41. PubMed PMID: 15667715.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrastructural localization of matrix metalloproteinase-9 in eosinophils from the cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis. AU - Tseng,Y K, AU - Tu,W C, AU - Lee,H H, AU - Chen,K M, AU - Chou,H L, AU - Lai,S C, PY - 2005/1/26/pubmed PY - 2005/4/26/medline PY - 2005/1/26/entrez SP - 831 EP - 41 JF - Annals of tropical medicine and parasitology JO - Ann Trop Med Parasitol VL - 98 IS - 8 N2 - Matrix metalloproteinase-9 (MMP-9) has been implicated in the pathogenesis of eosinophilic meningitis caused by Angiostrongylus cantonensis. In the present study, such meningitis in mice was found to be associated with elevated expression of MMP-9 mRNA, elevated MMP-9 concentrations and enhanced MMP-9 activity in the cerebrospinal fluid (CSF). Immunocytochemistry showed that an anti-MMP-9 antibody reacted with macrophages, neutrophils and eosinophils from the CSF. As eosinophils are generally considered to be effector cells in host defence against A. cantonensis infection, high-resolution immuno-electron microscopy was then used to confirm the localization of MMP-9 in the eosinophils from the CSF. The method used, which was based on immunogold, indicated that the eosinophilic MMP-9 was mostly localized in the 'small' granules in the cytoplasm and along the cell membrane, and not in the crystalloid-containing secretory granules observed. It therefore appears that MMP-9 is synthesised and/or stored in the small granules of the eosinophils, and is released into the subarachnoid space of the host's brain by secretion or cell rupture. SN - 0003-4983 UR - https://www.unboundmedicine.com/medline/citation/15667715/Ultrastructural_localization_of_matrix_metalloproteinase_9_in_eosinophils_from_the_cerebrospinal_fluid_of_mice_with_eosinophilic_meningitis_caused_by_Angiostrongylus_cantonensis_ L2 - https://medlineplus.gov/meningitis.html DB - PRIME DP - Unbound Medicine ER -