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Novel mutants of Erwinia carotovora subsp. carotovora defective in the production of plant cell wall degrading enzymes generated by Mu transpososome-mediated insertion mutagenesis.
FEMS Microbiol Lett. 2005 Feb 01; 243(1):93-9.FM

Abstract

As in Erwinia carotovora subsp. carotovora the regulation details of the main virulence factors, encoding extracellular enzymes that degrade the plant cell wall, is only rudimentally understood, we performed a genetic screen to identify novel candidate genes involved in the process. Initially, we used Mu transpososome-mediated mutagenesis approach to generate a comprehensive transposon insertion mutant library of ca. 10000 clones and screened the clones for the loss of extracellular enzyme production. Extracellular enzymes production was abolished by mutations in the chromosomal helEcc, trkAEcc yheLEcc, glsEcc, igaAEcc and cysQEcc genes. The findings reported here demonstrate that we have isolated six new representatives that belong to the pool of genes modulating the production of virulence factors in E. carotovora.

Authors+Show Affiliations

Department of Genetics, Institute of Molecular and Cell Biology, Tartu University, Estonian Biocenter, 23 Riia Street, Tartu 51010, Estonia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15668006

Citation

Laasik, Eve, et al. "Novel Mutants of Erwinia Carotovora Subsp. Carotovora Defective in the Production of Plant Cell Wall Degrading Enzymes Generated By Mu Transpososome-mediated Insertion Mutagenesis." FEMS Microbiology Letters, vol. 243, no. 1, 2005, pp. 93-9.
Laasik E, Ojarand M, Pajunen M, et al. Novel mutants of Erwinia carotovora subsp. carotovora defective in the production of plant cell wall degrading enzymes generated by Mu transpososome-mediated insertion mutagenesis. FEMS Microbiol Lett. 2005;243(1):93-9.
Laasik, E., Ojarand, M., Pajunen, M., Savilahti, H., & Mäe, A. (2005). Novel mutants of Erwinia carotovora subsp. carotovora defective in the production of plant cell wall degrading enzymes generated by Mu transpososome-mediated insertion mutagenesis. FEMS Microbiology Letters, 243(1), 93-9.
Laasik E, et al. Novel Mutants of Erwinia Carotovora Subsp. Carotovora Defective in the Production of Plant Cell Wall Degrading Enzymes Generated By Mu Transpososome-mediated Insertion Mutagenesis. FEMS Microbiol Lett. 2005 Feb 1;243(1):93-9. PubMed PMID: 15668006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel mutants of Erwinia carotovora subsp. carotovora defective in the production of plant cell wall degrading enzymes generated by Mu transpososome-mediated insertion mutagenesis. AU - Laasik,Eve, AU - Ojarand,Merli, AU - Pajunen,Maria, AU - Savilahti,Harri, AU - Mäe,Andres, PY - 2004/10/29/received PY - 2004/11/23/revised PY - 2004/11/25/accepted PY - 2005/1/26/pubmed PY - 2005/5/10/medline PY - 2005/1/26/entrez SP - 93 EP - 9 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 243 IS - 1 N2 - As in Erwinia carotovora subsp. carotovora the regulation details of the main virulence factors, encoding extracellular enzymes that degrade the plant cell wall, is only rudimentally understood, we performed a genetic screen to identify novel candidate genes involved in the process. Initially, we used Mu transpososome-mediated mutagenesis approach to generate a comprehensive transposon insertion mutant library of ca. 10000 clones and screened the clones for the loss of extracellular enzyme production. Extracellular enzymes production was abolished by mutations in the chromosomal helEcc, trkAEcc yheLEcc, glsEcc, igaAEcc and cysQEcc genes. The findings reported here demonstrate that we have isolated six new representatives that belong to the pool of genes modulating the production of virulence factors in E. carotovora. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/15668006/Novel_mutants_of_Erwinia_carotovora_subsp__carotovora_defective_in_the_production_of_plant_cell_wall_degrading_enzymes_generated_by_Mu_transpososome_mediated_insertion_mutagenesis_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1016/j.femsle.2004.11.045 DB - PRIME DP - Unbound Medicine ER -