Tags

Type your tag names separated by a space and hit enter

Compartment-specific role of the ascorbate-glutathione cycle in the response of tomato leaf cells to Botrytis cinerea infection.
J Exp Bot. 2005 Mar; 56(413):921-33.JE

Abstract

Changes in AA-GSH cycle activity following Botrytis cinerea infection were studied in tomato whole-leaf extracts as well as in chloroplasts, mitochondria, and peroxisomes. The oxidative effect of infection affected all cellular compartments although mitochondria and peroxisomes underwent the most pronounced changes. Apart from organelle-specific variations, a general shift of the cellular redox balance towards the oxidative state was found. It was manifested by the significant decline in concentrations and redox ratios of the ascorbate and glutathione pools as well as by the insufficient activity of MDHAR, DHAR, and GR needed for antioxidant regeneration. There was no compatibility between the ascorbate- and glutathione-mediated changes in different compartments. It was concluded that B. cinerea was able to break down the protective antioxidant barrier of the AA-GSH cycle at both the cellular and organellar levels. The changes in the AA-GSH cycle activity could partly be related to the B. cinerea-induced promotion of senescence that favoured disease progress.

Authors+Show Affiliations

Department of Plant Physiology and Biochemistry, University of Łódź, 90-237 Łódź, Banacha 12/16, Poland. elkuz@biol.uni.lodz.plNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15668222

Citation

Kuźniak, Elzbieta, and Maria Skłodowska. "Compartment-specific Role of the Ascorbate-glutathione Cycle in the Response of Tomato Leaf Cells to Botrytis Cinerea Infection." Journal of Experimental Botany, vol. 56, no. 413, 2005, pp. 921-33.
Kuźniak E, Skłodowska M. Compartment-specific role of the ascorbate-glutathione cycle in the response of tomato leaf cells to Botrytis cinerea infection. J Exp Bot. 2005;56(413):921-33.
Kuźniak, E., & Skłodowska, M. (2005). Compartment-specific role of the ascorbate-glutathione cycle in the response of tomato leaf cells to Botrytis cinerea infection. Journal of Experimental Botany, 56(413), 921-33.
Kuźniak E, Skłodowska M. Compartment-specific Role of the Ascorbate-glutathione Cycle in the Response of Tomato Leaf Cells to Botrytis Cinerea Infection. J Exp Bot. 2005;56(413):921-33. PubMed PMID: 15668222.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Compartment-specific role of the ascorbate-glutathione cycle in the response of tomato leaf cells to Botrytis cinerea infection. AU - Kuźniak,Elzbieta, AU - Skłodowska,Maria, Y1 - 2005/01/24/ PY - 2005/1/26/pubmed PY - 2005/6/9/medline PY - 2005/1/26/entrez SP - 921 EP - 33 JF - Journal of experimental botany JO - J Exp Bot VL - 56 IS - 413 N2 - Changes in AA-GSH cycle activity following Botrytis cinerea infection were studied in tomato whole-leaf extracts as well as in chloroplasts, mitochondria, and peroxisomes. The oxidative effect of infection affected all cellular compartments although mitochondria and peroxisomes underwent the most pronounced changes. Apart from organelle-specific variations, a general shift of the cellular redox balance towards the oxidative state was found. It was manifested by the significant decline in concentrations and redox ratios of the ascorbate and glutathione pools as well as by the insufficient activity of MDHAR, DHAR, and GR needed for antioxidant regeneration. There was no compatibility between the ascorbate- and glutathione-mediated changes in different compartments. It was concluded that B. cinerea was able to break down the protective antioxidant barrier of the AA-GSH cycle at both the cellular and organellar levels. The changes in the AA-GSH cycle activity could partly be related to the B. cinerea-induced promotion of senescence that favoured disease progress. SN - 0022-0957 UR - https://www.unboundmedicine.com/medline/citation/15668222/Compartment_specific_role_of_the_ascorbate_glutathione_cycle_in_the_response_of_tomato_leaf_cells_to_Botrytis_cinerea_infection_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/eri086 DB - PRIME DP - Unbound Medicine ER -