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Sample stacking for the analysis of eight penicillin antibiotics by micellar electrokinetic capillary chromatography.
Electrophoresis. 2005 Feb; 26(4-5):954-961.E

Abstract

We studied the use of micellar electrokinetic capillary chromatography for separating eight penicillins. The method consists of (i) an electrophoretic separation based on micellar electrokinetic capillary chromatography, which uses sodium dodecyl sulfate (SDS) as surfactant; (ii) a sample stacking technique called reverse electrode polarity stacking mode (REPSM); and (iii) direct UV detection. The background electrolyte that gave complete separation contained 20 mM sodium borate buffer and 60 mM SDS. The sensitivity of the method was improved by an enrichment step that used on-column stacking. The limits of detection were at the microg.L(-1) level for the penicillins and did not detract from the peak resolution.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Puig P
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain.
Borrull F
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain.
Calull M
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain.
Aguilar C
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain.

MeSH

Anti-Bacterial AgentsBoratesBuffersChromatography, Micellar Electrokinetic CapillaryElectrophoresis, CapillaryHydrogen-Ion ConcentrationPenicillinsPhosphatesSensitivity and SpecificitySodium Dodecyl Sulfate

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15669005

Citation

Puig, Patricia, et al. "Sample Stacking for the Analysis of Eight Penicillin Antibiotics By Micellar Electrokinetic Capillary Chromatography." Electrophoresis, vol. 26, no. 4-5, 2005, pp. 954-961.
Puig P, Borrull F, Calull M, et al. Sample stacking for the analysis of eight penicillin antibiotics by micellar electrokinetic capillary chromatography. Electrophoresis. 2005;26(4-5):954-961.
Puig, P., Borrull, F., Calull, M., & Aguilar, C. (2005). Sample stacking for the analysis of eight penicillin antibiotics by micellar electrokinetic capillary chromatography. Electrophoresis, 26(4-5), 954-961. https://doi.org/10.1002/elps.200406175
Puig P, et al. Sample Stacking for the Analysis of Eight Penicillin Antibiotics By Micellar Electrokinetic Capillary Chromatography. Electrophoresis. 2005;26(4-5):954-961. PubMed PMID: 15669005.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sample stacking for the analysis of eight penicillin antibiotics by micellar electrokinetic capillary chromatography. AU - Puig,Patricia, AU - Borrull,Francesc, AU - Calull,Marta, AU - Aguilar,Carme, PY - 2005/1/26/pubmed PY - 2005/7/27/medline PY - 2005/1/26/entrez SP - 954 EP - 961 JF - Electrophoresis JO - Electrophoresis VL - 26 IS - 4-5 N2 - We studied the use of micellar electrokinetic capillary chromatography for separating eight penicillins. The method consists of (i) an electrophoretic separation based on micellar electrokinetic capillary chromatography, which uses sodium dodecyl sulfate (SDS) as surfactant; (ii) a sample stacking technique called reverse electrode polarity stacking mode (REPSM); and (iii) direct UV detection. The background electrolyte that gave complete separation contained 20 mM sodium borate buffer and 60 mM SDS. The sensitivity of the method was improved by an enrichment step that used on-column stacking. The limits of detection were at the microg.L(-1) level for the penicillins and did not detract from the peak resolution. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15669005/Sample_stacking_for_the_analysis_of_eight_penicillin_antibiotics_by_micellar_electrokinetic_capillary_chromatography_ DB - PRIME DP - Unbound Medicine ER -