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Analysis of replication region of the cryptic plasmid pAG20 from Acetobacter aceti 3620.
Biochem Biophys Res Commun. 2005 Mar 04; 328(1):27-31.BB

Abstract

The DNA sequence of small cryptic plasmid pAG20 in Acetobacter aceti was determined at 3064 bp with 51.6% GC pairs. The plasmid encoded a 186 amino acid protein which is important for plasmid replication in Gram-negative bacteria except Escherichia coli. Two 21 bp large direct repeat sequence 1 and two 13 bp direct repeat sequence 2 were determined in the regulation region upstream from gene encoded Rep protein. Vector pAG24 with kanamycin gene and two deletion derivatives pAG25 and pAG26 without rep gene from plasmid pAG20 were constructed. Plasmid pAG24 was replicated in a broad host range like E. coli, Acetobacter pasteurianus, A. aceti, Comanomonas spp., Serratia marcescens, and Shigella spp.

Authors+Show Affiliations

Department of Molecular Biology, Comenius University, Faculty of Natural Sciences, Mlynska dolina B-2, 842 15 Bratislava 4, Slovak Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15670745

Citation

Kretová, Miroslava, et al. "Analysis of Replication Region of the Cryptic Plasmid pAG20 From Acetobacter Aceti 3620." Biochemical and Biophysical Research Communications, vol. 328, no. 1, 2005, pp. 27-31.
Kretová M, Szemes T, Laco J, et al. Analysis of replication region of the cryptic plasmid pAG20 from Acetobacter aceti 3620. Biochem Biophys Res Commun. 2005;328(1):27-31.
Kretová, M., Szemes, T., Laco, J., Gronesová, P., & Grones, J. (2005). Analysis of replication region of the cryptic plasmid pAG20 from Acetobacter aceti 3620. Biochemical and Biophysical Research Communications, 328(1), 27-31.
Kretová M, et al. Analysis of Replication Region of the Cryptic Plasmid pAG20 From Acetobacter Aceti 3620. Biochem Biophys Res Commun. 2005 Mar 4;328(1):27-31. PubMed PMID: 15670745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of replication region of the cryptic plasmid pAG20 from Acetobacter aceti 3620. AU - Kretová,Miroslava, AU - Szemes,Tomás, AU - Laco,Juraj, AU - Gronesová,Paulína, AU - Grones,Jozef, PY - 2004/12/16/received PY - 2005/1/27/pubmed PY - 2005/3/29/medline PY - 2005/1/27/entrez SP - 27 EP - 31 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 328 IS - 1 N2 - The DNA sequence of small cryptic plasmid pAG20 in Acetobacter aceti was determined at 3064 bp with 51.6% GC pairs. The plasmid encoded a 186 amino acid protein which is important for plasmid replication in Gram-negative bacteria except Escherichia coli. Two 21 bp large direct repeat sequence 1 and two 13 bp direct repeat sequence 2 were determined in the regulation region upstream from gene encoded Rep protein. Vector pAG24 with kanamycin gene and two deletion derivatives pAG25 and pAG26 without rep gene from plasmid pAG20 were constructed. Plasmid pAG24 was replicated in a broad host range like E. coli, Acetobacter pasteurianus, A. aceti, Comanomonas spp., Serratia marcescens, and Shigella spp. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/15670745/Analysis_of_replication_region_of_the_cryptic_plasmid_pAG20_from_Acetobacter_aceti_3620_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(04)02952-3 DB - PRIME DP - Unbound Medicine ER -