Tags

Type your tag names separated by a space and hit enter

Nutritional manipulation of primate retinas, III: Effects of lutein or zeaxanthin supplementation on adipose tissue and retina of xanthophyll-free monkeys.
Invest Ophthalmol Vis Sci. 2005 Feb; 46(2):692-702.IO

Abstract

PURPOSE

Macular pigment (MP) is composed of the xanthophylls lutein (L) and zeaxanthin (Z) and may help to prevent age-related macular degeneration or retard its progression. In this study the effects of L or Z supplementation on carotenoid levels was examined in serum, adipose tissue, and retina in rhesus monkeys with no previous intake of xanthophylls.

METHODS

From birth to 7 to 16 years of age, 18 rhesus monkeys were fed semipurified diets containing all essential nutrients but no xanthophylls. Six were supplemented with pure L and 6 with pure Z at 3.9 micromol/kg per day for 24 to 101 weeks. At baseline and at 4- to 12-week intervals, carotenoids in adipose tissue were measured by HPLC. At study completion, carotenoids in serum and retina (central 4 mm, 8-mm annulus, and the periphery) were determined. Results were compared with data from control monkeys fed a standard laboratory diet.

RESULTS

Monkeys fed xanthophyll-free diets had no L or Z in serum or tissues. After L or Z supplementation, serum and adipose tissue concentrations significantly increased in the supplemented groups. Both L and 3R,3'S-Z (RSZ or meso-Z, not present in the diet) were incorporated into retinas of monkeys supplemented with L, with RSZ present only in the macula (central 4 mm). All-trans Z, but no RSZ, accumulated in retinas of monkeys supplemented with Z.

CONCLUSIONS

L is the precursor of RSZ, a major component of macular pigment. Xanthophyll-free monkeys can accumulate retinal xanthophylls and provide a valuable model for examining their uptake and conversion.

Authors+Show Affiliations

Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, Massachusetts 02111, USA. elizabeth.johnson@tufts.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15671301

Citation

Johnson, Elizabeth J., et al. "Nutritional Manipulation of Primate Retinas, III: Effects of Lutein or Zeaxanthin Supplementation On Adipose Tissue and Retina of Xanthophyll-free Monkeys." Investigative Ophthalmology & Visual Science, vol. 46, no. 2, 2005, pp. 692-702.
Johnson EJ, Neuringer M, Russell RM, et al. Nutritional manipulation of primate retinas, III: Effects of lutein or zeaxanthin supplementation on adipose tissue and retina of xanthophyll-free monkeys. Invest Ophthalmol Vis Sci. 2005;46(2):692-702.
Johnson, E. J., Neuringer, M., Russell, R. M., Schalch, W., & Snodderly, D. M. (2005). Nutritional manipulation of primate retinas, III: Effects of lutein or zeaxanthin supplementation on adipose tissue and retina of xanthophyll-free monkeys. Investigative Ophthalmology & Visual Science, 46(2), 692-702.
Johnson EJ, et al. Nutritional Manipulation of Primate Retinas, III: Effects of Lutein or Zeaxanthin Supplementation On Adipose Tissue and Retina of Xanthophyll-free Monkeys. Invest Ophthalmol Vis Sci. 2005;46(2):692-702. PubMed PMID: 15671301.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nutritional manipulation of primate retinas, III: Effects of lutein or zeaxanthin supplementation on adipose tissue and retina of xanthophyll-free monkeys. AU - Johnson,Elizabeth J, AU - Neuringer,Martha, AU - Russell,Robert M, AU - Schalch,Wolfgang, AU - Snodderly,D Max, PY - 2005/1/27/pubmed PY - 2005/3/12/medline PY - 2005/1/27/entrez SP - 692 EP - 702 JF - Investigative ophthalmology & visual science JO - Invest. Ophthalmol. Vis. Sci. VL - 46 IS - 2 N2 - PURPOSE: Macular pigment (MP) is composed of the xanthophylls lutein (L) and zeaxanthin (Z) and may help to prevent age-related macular degeneration or retard its progression. In this study the effects of L or Z supplementation on carotenoid levels was examined in serum, adipose tissue, and retina in rhesus monkeys with no previous intake of xanthophylls. METHODS: From birth to 7 to 16 years of age, 18 rhesus monkeys were fed semipurified diets containing all essential nutrients but no xanthophylls. Six were supplemented with pure L and 6 with pure Z at 3.9 micromol/kg per day for 24 to 101 weeks. At baseline and at 4- to 12-week intervals, carotenoids in adipose tissue were measured by HPLC. At study completion, carotenoids in serum and retina (central 4 mm, 8-mm annulus, and the periphery) were determined. Results were compared with data from control monkeys fed a standard laboratory diet. RESULTS: Monkeys fed xanthophyll-free diets had no L or Z in serum or tissues. After L or Z supplementation, serum and adipose tissue concentrations significantly increased in the supplemented groups. Both L and 3R,3'S-Z (RSZ or meso-Z, not present in the diet) were incorporated into retinas of monkeys supplemented with L, with RSZ present only in the macula (central 4 mm). All-trans Z, but no RSZ, accumulated in retinas of monkeys supplemented with Z. CONCLUSIONS: L is the precursor of RSZ, a major component of macular pigment. Xanthophyll-free monkeys can accumulate retinal xanthophylls and provide a valuable model for examining their uptake and conversion. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/15671301/Nutritional_manipulation_of_primate_retinas_III:_Effects_of_lutein_or_zeaxanthin_supplementation_on_adipose_tissue_and_retina_of_xanthophyll_free_monkeys_ L2 - http://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.02-1192 DB - PRIME DP - Unbound Medicine ER -