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[Frequency of intestinal microsporidian infections in HIV-positive patients, as diagnosis by quick hot Gram chromotrope staining and PCR].
Biomedica. 2004 Dec; 24(4):375-84.B

Abstract

Microsporidia are intracellular obligate parasites, today mainly associated with diarrhea in AIDS patients. Microsporidia prevalence ranges from 8% to 52% in different countries, as evaluated by several diagnostic methods, such as the stain test and PCR. In Medellín, Colombia, its frequency is unknown, and hence, a study was undertaken to determine the frequency of intestinal microsporidiosis in HIV patients, by means of the quick-hot Gram chromotrope test and the PCR. A prospective and descriptive study of an intentional population of all HIV-positive patients was sent to the Grupo Interdisciplinario para el Estudio de las Parasitosis Intestinales laboratory by institutions treating the HIV-positive patients of Medellín between August 2001 and September 2002. The clinical-epidemiological survey included a serial stool test with direct concentration and special stains for coccidiae and intestinal microsporidia. In addition, counts of lymphocytes TCD4+ and viral load were requested. One hundred and three patients with ages ranging from 2-74 years were evaluated. Seventy percent presented with diarrhea--mostly in men (83.5%). The overall frequency of intestinal microsporidiosis was 3.9% and that of other intestinal parasitic infections was 39.8%. Three of the four patients positive for microsporida were infected with Enterocytozoon bieneusi and one with Encephalitozoon intestinalis. The microsporidiosis frequency was relatively low with 3 of the 4 cases associated with protracted diarrhea, counts of LTCD4+ below 100 cel/microl and viral loads up to 100,000 copies.

Authors+Show Affiliations

Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia. jbotero@quimbaya.udea.edu.coNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

15678801

Citation

Botero, Jorge H., et al. "[Frequency of Intestinal Microsporidian Infections in HIV-positive Patients, as Diagnosis By Quick Hot Gram Chromotrope Staining and PCR]." Biomedica : Revista Del Instituto Nacional De Salud, vol. 24, no. 4, 2004, pp. 375-84.
Botero JH, Montoya MN, Vanegas AL, et al. [Frequency of intestinal microsporidian infections in HIV-positive patients, as diagnosis by quick hot Gram chromotrope staining and PCR]. Biomedica. 2004;24(4):375-84.
Botero, J. H., Montoya, M. N., Vanegas, A. L., Díaz, A., Navarro-i-Martínez, L., Bornay, F. J., Izquierdo, F., del Aguila, C., & Agudelo, S. d. e. l. . P. (2004). [Frequency of intestinal microsporidian infections in HIV-positive patients, as diagnosis by quick hot Gram chromotrope staining and PCR]. Biomedica : Revista Del Instituto Nacional De Salud, 24(4), 375-84.
Botero JH, et al. [Frequency of Intestinal Microsporidian Infections in HIV-positive Patients, as Diagnosis By Quick Hot Gram Chromotrope Staining and PCR]. Biomedica. 2004;24(4):375-84. PubMed PMID: 15678801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Frequency of intestinal microsporidian infections in HIV-positive patients, as diagnosis by quick hot Gram chromotrope staining and PCR]. AU - Botero,Jorge H, AU - Montoya,Martha Nelly, AU - Vanegas,Adriana Lucía, AU - Díaz,Abel, AU - Navarro-i-Martínez,Luis, AU - Bornay,Fernando Jorge, AU - Izquierdo,Fernando, AU - del Aguila,Carmen, AU - Agudelo,Sonia del Pilar, PY - 2005/2/1/pubmed PY - 2005/2/16/medline PY - 2005/2/1/entrez SP - 375 EP - 84 JF - Biomedica : revista del Instituto Nacional de Salud JO - Biomedica VL - 24 IS - 4 N2 - Microsporidia are intracellular obligate parasites, today mainly associated with diarrhea in AIDS patients. Microsporidia prevalence ranges from 8% to 52% in different countries, as evaluated by several diagnostic methods, such as the stain test and PCR. In Medellín, Colombia, its frequency is unknown, and hence, a study was undertaken to determine the frequency of intestinal microsporidiosis in HIV patients, by means of the quick-hot Gram chromotrope test and the PCR. A prospective and descriptive study of an intentional population of all HIV-positive patients was sent to the Grupo Interdisciplinario para el Estudio de las Parasitosis Intestinales laboratory by institutions treating the HIV-positive patients of Medellín between August 2001 and September 2002. The clinical-epidemiological survey included a serial stool test with direct concentration and special stains for coccidiae and intestinal microsporidia. In addition, counts of lymphocytes TCD4+ and viral load were requested. One hundred and three patients with ages ranging from 2-74 years were evaluated. Seventy percent presented with diarrhea--mostly in men (83.5%). The overall frequency of intestinal microsporidiosis was 3.9% and that of other intestinal parasitic infections was 39.8%. Three of the four patients positive for microsporida were infected with Enterocytozoon bieneusi and one with Encephalitozoon intestinalis. The microsporidiosis frequency was relatively low with 3 of the 4 cases associated with protracted diarrhea, counts of LTCD4+ below 100 cel/microl and viral loads up to 100,000 copies. SN - 0120-4157 UR - https://www.unboundmedicine.com/medline/citation/15678801/[Frequency_of_intestinal_microsporidian_infections_in_HIV_positive_patients_as_diagnosis_by_quick_hot_Gram_chromotrope_staining_and_PCR]_ L2 - http://www.diseaseinfosearch.org/result/9735 DB - PRIME DP - Unbound Medicine ER -