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Analysis of keratan sulfate oligosaccharides by electrospray ionization tandem mass spectrometry.
Anal Chem. 2005 Feb 01; 77(3):902-10.AC

Abstract

Keratan sulfate (KS) is a glycosaminoglycan consisting of repeating disaccharide units composed of alternating residues of d-galactose and N-acetyl-d-glucosamine linked beta-(1-4) and beta-(1-3), respectively. In this study, electrospray ionization tandem mass spectrometry (ESI-MS/MS) was employed to identify keratan sulfate oligosaccharides. Two nonsulfated disaccharide isomers and two monosulfated disaccharide isomers were distinguished through MS/MS. In MS(1) spectra of multiply sulfated KS oligosaccharides, the charge state of the most abundant molecular ion equals the number of sulfates. Subsequent MS(2) and MS(3) spectra of mono-, di-, tri-, and tetrasulfated KS oligosaccharides and sialylated tetrasaccharides reveal diagnostic ions that can be used as fingerprint maps to identify unknown KS oligosaccharides. Based on the pattern of fragment ions, the compositions of an oligosaccharide mixture from shark cartilage KS and of two enzyme digests of bovine corneal KS were determined directly, without prior isolation of individual oligosaccharides by HPLC or other methods.

Authors+Show Affiliations

Zhang Y
Division of Biology, Kansas State University, Manhattan, KS 66506, USA. ytz@ksu.edu
Kariya Y
No affiliation info available
Conrad AH
No affiliation info available
Tasheva ES
No affiliation info available
Conrad GW
No affiliation info available

MeSH

AcetylglucosamineAnimalsCarbohydrate SequenceCattleCorneaGalactoseIsomerismKeratan SulfateMolecular Sequence DataOligosaccharidesSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15679360

Citation

Zhang, Yuntao, et al. "Analysis of Keratan Sulfate Oligosaccharides By Electrospray Ionization Tandem Mass Spectrometry." Analytical Chemistry, vol. 77, no. 3, 2005, pp. 902-10.
Zhang Y, Kariya Y, Conrad AH, et al. Analysis of keratan sulfate oligosaccharides by electrospray ionization tandem mass spectrometry. Anal Chem. 2005;77(3):902-10.
Zhang, Y., Kariya, Y., Conrad, A. H., Tasheva, E. S., & Conrad, G. W. (2005). Analysis of keratan sulfate oligosaccharides by electrospray ionization tandem mass spectrometry. Analytical Chemistry, 77(3), 902-10.
Zhang Y, et al. Analysis of Keratan Sulfate Oligosaccharides By Electrospray Ionization Tandem Mass Spectrometry. Anal Chem. 2005 Feb 1;77(3):902-10. PubMed PMID: 15679360.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of keratan sulfate oligosaccharides by electrospray ionization tandem mass spectrometry. AU - Zhang,Yuntao, AU - Kariya,Yutaka, AU - Conrad,Abigail H, AU - Tasheva,Elena S, AU - Conrad,Gary W, PY - 2005/2/1/pubmed PY - 2005/5/6/medline PY - 2005/2/1/entrez SP - 902 EP - 10 JF - Analytical chemistry JO - Anal Chem VL - 77 IS - 3 N2 - Keratan sulfate (KS) is a glycosaminoglycan consisting of repeating disaccharide units composed of alternating residues of d-galactose and N-acetyl-d-glucosamine linked beta-(1-4) and beta-(1-3), respectively. In this study, electrospray ionization tandem mass spectrometry (ESI-MS/MS) was employed to identify keratan sulfate oligosaccharides. Two nonsulfated disaccharide isomers and two monosulfated disaccharide isomers were distinguished through MS/MS. In MS(1) spectra of multiply sulfated KS oligosaccharides, the charge state of the most abundant molecular ion equals the number of sulfates. Subsequent MS(2) and MS(3) spectra of mono-, di-, tri-, and tetrasulfated KS oligosaccharides and sialylated tetrasaccharides reveal diagnostic ions that can be used as fingerprint maps to identify unknown KS oligosaccharides. Based on the pattern of fragment ions, the compositions of an oligosaccharide mixture from shark cartilage KS and of two enzyme digests of bovine corneal KS were determined directly, without prior isolation of individual oligosaccharides by HPLC or other methods. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15679360/Analysis_of_keratan_sulfate_oligosaccharides_by_electrospray_ionization_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -