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AM404, an inhibitor of anandamide reuptake decreases Fos-immunoreactivity in the spinal cord of neuropathic rats after non-noxious stimulation.
Eur J Pharmacol. 2005 Jan 31; 508(1-3):139-46.EJ

Abstract

Cannabinoids like anandamide are involved in pain transmission. In this study we evaluated the effects of administrating N-(4-hydroxyphenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide (AM404), an inhibitor of anandamide reuptake and monitoring the expression of c-fos, a marker of activated neurons in an experimental model of neuropathic pain (sciatic nerve tying). Fos expression was monitored 14 days after tying of sciatic nerve and 2 h after non-noxious stimulation. We showed that non-noxious stimulation increased Fos-positivity in the dorsal superficial laminae of the lumbar spinal cord of tied animals but not in the control animals. AM404 significantly reduced Fos induction in tied animals. Co-administration of cannabinoid CB1 receptor, cannabinoid CB2 receptor and transient receptor potential vanilloid type 1 (TRPV-1) antagonists reduced the effect of AM404 and this reduction was higher using cannabinoid CB1 receptor antagonist. These results suggest that AM404 could be a useful drug to reduce neuropathic pain and that cannabinoid CB1 receptor, cannabinoid CB2 receptor and vanilloid TRPV-1 receptor are involved.

Authors+Show Affiliations

Department of Biomedical Sciences and Biotechnology, Division of Human Anatomy, University of Brescia, viale Europa 11, 25123 Brescia, Italy. rodella@med.unibs.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15680264

Citation

Rodella, Luigi F., et al. "AM404, an Inhibitor of Anandamide Reuptake Decreases Fos-immunoreactivity in the Spinal Cord of Neuropathic Rats After Non-noxious Stimulation." European Journal of Pharmacology, vol. 508, no. 1-3, 2005, pp. 139-46.
Rodella LF, Borsani E, Rezzani R, et al. AM404, an inhibitor of anandamide reuptake decreases Fos-immunoreactivity in the spinal cord of neuropathic rats after non-noxious stimulation. Eur J Pharmacol. 2005;508(1-3):139-46.
Rodella, L. F., Borsani, E., Rezzani, R., Ricci, F., Buffoli, B., & Bianchi, R. (2005). AM404, an inhibitor of anandamide reuptake decreases Fos-immunoreactivity in the spinal cord of neuropathic rats after non-noxious stimulation. European Journal of Pharmacology, 508(1-3), 139-46.
Rodella LF, et al. AM404, an Inhibitor of Anandamide Reuptake Decreases Fos-immunoreactivity in the Spinal Cord of Neuropathic Rats After Non-noxious Stimulation. Eur J Pharmacol. 2005 Jan 31;508(1-3):139-46. PubMed PMID: 15680264.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - AM404, an inhibitor of anandamide reuptake decreases Fos-immunoreactivity in the spinal cord of neuropathic rats after non-noxious stimulation. AU - Rodella,Luigi F, AU - Borsani,Elisa, AU - Rezzani,Rita, AU - Ricci,Francesca, AU - Buffoli,Barbara, AU - Bianchi,Rossella, Y1 - 2005/01/12/ PY - 2004/09/21/received PY - 2004/12/14/revised PY - 2004/12/17/accepted PY - 2005/2/1/pubmed PY - 2005/7/15/medline PY - 2005/2/1/entrez SP - 139 EP - 46 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 508 IS - 1-3 N2 - Cannabinoids like anandamide are involved in pain transmission. In this study we evaluated the effects of administrating N-(4-hydroxyphenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide (AM404), an inhibitor of anandamide reuptake and monitoring the expression of c-fos, a marker of activated neurons in an experimental model of neuropathic pain (sciatic nerve tying). Fos expression was monitored 14 days after tying of sciatic nerve and 2 h after non-noxious stimulation. We showed that non-noxious stimulation increased Fos-positivity in the dorsal superficial laminae of the lumbar spinal cord of tied animals but not in the control animals. AM404 significantly reduced Fos induction in tied animals. Co-administration of cannabinoid CB1 receptor, cannabinoid CB2 receptor and transient receptor potential vanilloid type 1 (TRPV-1) antagonists reduced the effect of AM404 and this reduction was higher using cannabinoid CB1 receptor antagonist. These results suggest that AM404 could be a useful drug to reduce neuropathic pain and that cannabinoid CB1 receptor, cannabinoid CB2 receptor and vanilloid TRPV-1 receptor are involved. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/15680264/AM404_an_inhibitor_of_anandamide_reuptake_decreases_Fos_immunoreactivity_in_the_spinal_cord_of_neuropathic_rats_after_non_noxious_stimulation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(04)01446-3 DB - PRIME DP - Unbound Medicine ER -