Tags

Type your tag names separated by a space and hit enter

Salvianic acid A protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity.
Neurosci Res. 2005 Feb; 51(2):129-38.NR

Abstract

1-methyl-4-phenylpyridinium ion (MPP(+)), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin because it elicits a severe Parkinson's disease-like syndrome with elevation of intracellular reactive oxygen species (ROS) level and apoptotic death. Salvianic acid A (SA), isolated from the Chinese herbal medicine Salvia miltiorrhiza, is capable of protecting diverse kinds of cells from damage caused by a variety of toxic stimuli. In the present study, we investigated the protective effects of SA on MPP(+)-induced cytotoxicity in human neuroblastoma SH-SY5Y cells, as well as the underlying mechanism. Treatment of SH-SY5Y cells with MPP(+) caused the loss of cell viability, and condensation and fragmentation of nuclei, which was associated with the elevation of ROS level, the increase in Bax/Bcl-2 ratio, and the activation of caspase-3. MPP(+) induced mitochondria dysfunction characterized by mitochondrial membrane potential loss and cytochrome c release. These phenotypes induced by MPP(+) were reversed by SA. Our results suggested that the protective effects of SA on MPP(+)-induced cytotoxicity may be ascribed to its antioxidative properties and anti-apoptotic activity via regulating the expression of Bcl-2 and Bax. These data indicated that SA might provide a useful therapeutic strategy for the treatment of progressive neurodegenerative disease such as Parkinson's disease.

Authors+Show Affiliations

Wang XJ
Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.
Xu JX
No affiliation info available

MeSH

1-Methyl-4-phenylpyridiniumApoptosisBisbenzimidazoleBlotting, WesternCaffeic AcidsCaspase 3CaspasesCatalaseCell Line, TumorCell NucleusCell SurvivalCytochromes cDithioerythritolDose-Response Relationship, DrugDrug InteractionsFlow CytometryHumansLactatesMembrane PotentialsMitochondriaNecrosisNeuroblastomaNeuroprotective AgentsProto-Oncogene Proteins c-bcl-2Reactive Oxygen SpeciesSulfhydryl ReagentsSuperoxide Dismutasebcl-2-Associated X Protein

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15681030

Citation

Wang, Xin-Jian, and Jian-Xing Xu. "Salvianic Acid a Protects Human Neuroblastoma SH-SY5Y Cells Against MPP+-induced Cytotoxicity." Neuroscience Research, vol. 51, no. 2, 2005, pp. 129-38.
Wang XJ, Xu JX. Salvianic acid A protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. Neurosci Res. 2005;51(2):129-38.
Wang, X. J., & Xu, J. X. (2005). Salvianic acid A protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. Neuroscience Research, 51(2), 129-38.
Wang XJ, Xu JX. Salvianic Acid a Protects Human Neuroblastoma SH-SY5Y Cells Against MPP+-induced Cytotoxicity. Neurosci Res. 2005;51(2):129-38. PubMed PMID: 15681030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Salvianic acid A protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. AU - Wang,Xin-Jian, AU - Xu,Jian-Xing, PY - 2004/07/04/received PY - 2004/10/13/accepted PY - 2005/2/1/pubmed PY - 2005/5/3/medline PY - 2005/2/1/entrez SP - 129 EP - 38 JF - Neuroscience research JO - Neurosci Res VL - 51 IS - 2 N2 - 1-methyl-4-phenylpyridinium ion (MPP(+)), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin because it elicits a severe Parkinson's disease-like syndrome with elevation of intracellular reactive oxygen species (ROS) level and apoptotic death. Salvianic acid A (SA), isolated from the Chinese herbal medicine Salvia miltiorrhiza, is capable of protecting diverse kinds of cells from damage caused by a variety of toxic stimuli. In the present study, we investigated the protective effects of SA on MPP(+)-induced cytotoxicity in human neuroblastoma SH-SY5Y cells, as well as the underlying mechanism. Treatment of SH-SY5Y cells with MPP(+) caused the loss of cell viability, and condensation and fragmentation of nuclei, which was associated with the elevation of ROS level, the increase in Bax/Bcl-2 ratio, and the activation of caspase-3. MPP(+) induced mitochondria dysfunction characterized by mitochondrial membrane potential loss and cytochrome c release. These phenotypes induced by MPP(+) were reversed by SA. Our results suggested that the protective effects of SA on MPP(+)-induced cytotoxicity may be ascribed to its antioxidative properties and anti-apoptotic activity via regulating the expression of Bcl-2 and Bax. These data indicated that SA might provide a useful therapeutic strategy for the treatment of progressive neurodegenerative disease such as Parkinson's disease. SN - 0168-0102 UR - https://www.unboundmedicine.com/medline/citation/15681030/Salvianic_acid_A_protects_human_neuroblastoma_SH_SY5Y_cells_against_MPP+_induced_cytotoxicity_ DB - PRIME DP - Unbound Medicine ER -