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Salvianic acid A protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity.
Neurosci Res. 2005 Feb; 51(2):129-38.NR

Abstract

1-methyl-4-phenylpyridinium ion (MPP(+)), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin because it elicits a severe Parkinson's disease-like syndrome with elevation of intracellular reactive oxygen species (ROS) level and apoptotic death. Salvianic acid A (SA), isolated from the Chinese herbal medicine Salvia miltiorrhiza, is capable of protecting diverse kinds of cells from damage caused by a variety of toxic stimuli. In the present study, we investigated the protective effects of SA on MPP(+)-induced cytotoxicity in human neuroblastoma SH-SY5Y cells, as well as the underlying mechanism. Treatment of SH-SY5Y cells with MPP(+) caused the loss of cell viability, and condensation and fragmentation of nuclei, which was associated with the elevation of ROS level, the increase in Bax/Bcl-2 ratio, and the activation of caspase-3. MPP(+) induced mitochondria dysfunction characterized by mitochondrial membrane potential loss and cytochrome c release. These phenotypes induced by MPP(+) were reversed by SA. Our results suggested that the protective effects of SA on MPP(+)-induced cytotoxicity may be ascribed to its antioxidative properties and anti-apoptotic activity via regulating the expression of Bcl-2 and Bax. These data indicated that SA might provide a useful therapeutic strategy for the treatment of progressive neurodegenerative disease such as Parkinson's disease.

Authors+Show Affiliations

Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15681030

Citation

Wang, Xin-Jian, and Jian-Xing Xu. "Salvianic Acid a Protects Human Neuroblastoma SH-SY5Y Cells Against MPP+-induced Cytotoxicity." Neuroscience Research, vol. 51, no. 2, 2005, pp. 129-38.
Wang XJ, Xu JX. Salvianic acid A protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. Neurosci Res. 2005;51(2):129-38.
Wang, X. J., & Xu, J. X. (2005). Salvianic acid A protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. Neuroscience Research, 51(2), 129-38.
Wang XJ, Xu JX. Salvianic Acid a Protects Human Neuroblastoma SH-SY5Y Cells Against MPP+-induced Cytotoxicity. Neurosci Res. 2005;51(2):129-38. PubMed PMID: 15681030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Salvianic acid A protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. AU - Wang,Xin-Jian, AU - Xu,Jian-Xing, PY - 2004/07/04/received PY - 2004/10/13/accepted PY - 2005/2/1/pubmed PY - 2005/5/3/medline PY - 2005/2/1/entrez SP - 129 EP - 38 JF - Neuroscience research JO - Neurosci Res VL - 51 IS - 2 N2 - 1-methyl-4-phenylpyridinium ion (MPP(+)), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin because it elicits a severe Parkinson's disease-like syndrome with elevation of intracellular reactive oxygen species (ROS) level and apoptotic death. Salvianic acid A (SA), isolated from the Chinese herbal medicine Salvia miltiorrhiza, is capable of protecting diverse kinds of cells from damage caused by a variety of toxic stimuli. In the present study, we investigated the protective effects of SA on MPP(+)-induced cytotoxicity in human neuroblastoma SH-SY5Y cells, as well as the underlying mechanism. Treatment of SH-SY5Y cells with MPP(+) caused the loss of cell viability, and condensation and fragmentation of nuclei, which was associated with the elevation of ROS level, the increase in Bax/Bcl-2 ratio, and the activation of caspase-3. MPP(+) induced mitochondria dysfunction characterized by mitochondrial membrane potential loss and cytochrome c release. These phenotypes induced by MPP(+) were reversed by SA. Our results suggested that the protective effects of SA on MPP(+)-induced cytotoxicity may be ascribed to its antioxidative properties and anti-apoptotic activity via regulating the expression of Bcl-2 and Bax. These data indicated that SA might provide a useful therapeutic strategy for the treatment of progressive neurodegenerative disease such as Parkinson's disease. SN - 0168-0102 UR - https://www.unboundmedicine.com/medline/citation/15681030/Salvianic_acid_A_protects_human_neuroblastoma_SH_SY5Y_cells_against_MPP+_induced_cytotoxicity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-0102(04)00255-X DB - PRIME DP - Unbound Medicine ER -