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Identification and functional analysis of Salmonella enterica serovar Typhimurium PmrA-regulated genes.
FEMS Immunol Med Microbiol. 2005 Feb 01; 43(2):249-58.FI

Abstract

The PmrA-PmrB two-component regulatory system of Salmonella enterica serovar Typhimurium is activated in vivo and plays an important role in resistance to cationic antimicrobial peptides. Resistance is partly mediated by modifications to the lipopolysaccharide. To identify new PmrA-regulated genes, microarray analysis was undertaken comparing cDNA derived from PmrA-constitutive and PmrA-null strains. A combination of RT-PCR and transcriptional analysis confirmed the inclusion of six new loci in the PmrA-PmrB regulon: STM1253, STM1269, STM4118, STM0459, STM3968 and STM4568. These loci did not affect the ability to grow in high iron conditions, the ability to modify lipid A with aminoarabinose, or virulence. STM4118, a putative phosphoethanolamine phosphotransferase, had a minor effect on polymyxin resistance, whereas the remaining genes had no role in polymyxin resistance. Although several of the identified loci lacked the consensus PmrA binding site, PmrA was demonstrated to bind the promoter of a PmrA-activated gene lacking the consensus site. A more complete definition of the PmrA-PmrB regulon will provide a better understanding of its role in host and non-host environments.

Authors+Show Affiliations

Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, MC 7758, San Antonio, TX 78229-3900, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15681155

Citation

Tamayo, Rita, et al. "Identification and Functional Analysis of Salmonella Enterica Serovar Typhimurium PmrA-regulated Genes." FEMS Immunology and Medical Microbiology, vol. 43, no. 2, 2005, pp. 249-58.
Tamayo R, Prouty AM, Gunn JS. Identification and functional analysis of Salmonella enterica serovar Typhimurium PmrA-regulated genes. FEMS Immunol Med Microbiol. 2005;43(2):249-58.
Tamayo, R., Prouty, A. M., & Gunn, J. S. (2005). Identification and functional analysis of Salmonella enterica serovar Typhimurium PmrA-regulated genes. FEMS Immunology and Medical Microbiology, 43(2), 249-58.
Tamayo R, Prouty AM, Gunn JS. Identification and Functional Analysis of Salmonella Enterica Serovar Typhimurium PmrA-regulated Genes. FEMS Immunol Med Microbiol. 2005 Feb 1;43(2):249-58. PubMed PMID: 15681155.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and functional analysis of Salmonella enterica serovar Typhimurium PmrA-regulated genes. AU - Tamayo,Rita, AU - Prouty,Angela M, AU - Gunn,John S, PY - 2004/06/23/received PY - 2004/08/10/revised PY - 2004/08/23/accepted PY - 2005/2/1/pubmed PY - 2005/8/5/medline PY - 2005/2/1/entrez SP - 249 EP - 58 JF - FEMS immunology and medical microbiology JO - FEMS Immunol Med Microbiol VL - 43 IS - 2 N2 - The PmrA-PmrB two-component regulatory system of Salmonella enterica serovar Typhimurium is activated in vivo and plays an important role in resistance to cationic antimicrobial peptides. Resistance is partly mediated by modifications to the lipopolysaccharide. To identify new PmrA-regulated genes, microarray analysis was undertaken comparing cDNA derived from PmrA-constitutive and PmrA-null strains. A combination of RT-PCR and transcriptional analysis confirmed the inclusion of six new loci in the PmrA-PmrB regulon: STM1253, STM1269, STM4118, STM0459, STM3968 and STM4568. These loci did not affect the ability to grow in high iron conditions, the ability to modify lipid A with aminoarabinose, or virulence. STM4118, a putative phosphoethanolamine phosphotransferase, had a minor effect on polymyxin resistance, whereas the remaining genes had no role in polymyxin resistance. Although several of the identified loci lacked the consensus PmrA binding site, PmrA was demonstrated to bind the promoter of a PmrA-activated gene lacking the consensus site. A more complete definition of the PmrA-PmrB regulon will provide a better understanding of its role in host and non-host environments. SN - 0928-8244 UR - https://www.unboundmedicine.com/medline/citation/15681155/Identification_and_functional_analysis_of_Salmonella_enterica_serovar_Typhimurium_PmrA_regulated_genes_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0928-8244&date=2005&volume=43&issue=2&spage=249 DB - PRIME DP - Unbound Medicine ER -