Tags

Type your tag names separated by a space and hit enter

Rapid genotypic detection of Bacillus anthracis and the Bacillus cereus group by multiplex real-time PCR melting curve analysis.
FEMS Immunol Med Microbiol 2005; 43(2):301-10FI

Abstract

Bacillus anthracis has four plasmid possible virulence genotypes: pXO1+/pXO2+, pXO1+/pXO2-, pXO1-/pXO2+ or pXO1-/pXO2-. Due to the lack of a specific chromosomal marker for B. anthracis, differentiation of the pXO1-/pXO2- form of B. anthracis from closely related Bacillus cereus group species is difficult. In this study, we evaluate the ability of sspE, pXO1 and pXO2 primers to discriminate individual B. anthracis and the B. cereus group genotypes using multiplex real-time PCR and melting curve analysis. Optimal conditions for successful multiplex assays have been established. Purified DNAs from 38 bacterial strains including 11 strains of B. anthracis and 18 B. cereus group strains were analyzed. Nine of the B. cereus group near-neighbor strains were shown by multilocus sequence typing to be phylogenetically proximate to the B. anthracis clade. We have demonstrated that the four plasmid genotypes of B. anthracis and B. cereus group near-neighbors were differentially and simultaneously discriminated by this assay.

Authors+Show Affiliations

Department of Microbiology, College of Medicine, Chung-Ang Univerisity, 221, Heuksuk-dong, Dongjak-gu, Seoul 156-756, South Korea. kimkj@cau.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15681162

Citation

Kim, Kijeong, et al. "Rapid Genotypic Detection of Bacillus Anthracis and the Bacillus Cereus Group By Multiplex Real-time PCR Melting Curve Analysis." FEMS Immunology and Medical Microbiology, vol. 43, no. 2, 2005, pp. 301-10.
Kim K, Seo J, Wheeler K, et al. Rapid genotypic detection of Bacillus anthracis and the Bacillus cereus group by multiplex real-time PCR melting curve analysis. FEMS Immunol Med Microbiol. 2005;43(2):301-10.
Kim, K., Seo, J., Wheeler, K., Park, C., Kim, D., Park, S., ... Leighton, T. (2005). Rapid genotypic detection of Bacillus anthracis and the Bacillus cereus group by multiplex real-time PCR melting curve analysis. FEMS Immunology and Medical Microbiology, 43(2), pp. 301-10.
Kim K, et al. Rapid Genotypic Detection of Bacillus Anthracis and the Bacillus Cereus Group By Multiplex Real-time PCR Melting Curve Analysis. FEMS Immunol Med Microbiol. 2005 Feb 1;43(2):301-10. PubMed PMID: 15681162.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid genotypic detection of Bacillus anthracis and the Bacillus cereus group by multiplex real-time PCR melting curve analysis. AU - Kim,Kijeong, AU - Seo,Juwon, AU - Wheeler,Katherine, AU - Park,Chulmin, AU - Kim,Daewhan, AU - Park,Seungjoon, AU - Kim,Wonyong, AU - Chung,Sang-In, AU - Leighton,Terrance, PY - 2004/06/05/received PY - 2004/10/10/revised PY - 2004/10/18/accepted PY - 2005/2/1/pubmed PY - 2005/8/5/medline PY - 2005/2/1/entrez SP - 301 EP - 10 JF - FEMS immunology and medical microbiology JO - FEMS Immunol. Med. Microbiol. VL - 43 IS - 2 N2 - Bacillus anthracis has four plasmid possible virulence genotypes: pXO1+/pXO2+, pXO1+/pXO2-, pXO1-/pXO2+ or pXO1-/pXO2-. Due to the lack of a specific chromosomal marker for B. anthracis, differentiation of the pXO1-/pXO2- form of B. anthracis from closely related Bacillus cereus group species is difficult. In this study, we evaluate the ability of sspE, pXO1 and pXO2 primers to discriminate individual B. anthracis and the B. cereus group genotypes using multiplex real-time PCR and melting curve analysis. Optimal conditions for successful multiplex assays have been established. Purified DNAs from 38 bacterial strains including 11 strains of B. anthracis and 18 B. cereus group strains were analyzed. Nine of the B. cereus group near-neighbor strains were shown by multilocus sequence typing to be phylogenetically proximate to the B. anthracis clade. We have demonstrated that the four plasmid genotypes of B. anthracis and B. cereus group near-neighbors were differentially and simultaneously discriminated by this assay. SN - 0928-8244 UR - https://www.unboundmedicine.com/medline/citation/15681162/Rapid_genotypic_detection_of_Bacillus_anthracis_and_the_Bacillus_cereus_group_by_multiplex_real_time_PCR_melting_curve_analysis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0928-8244&date=2005&volume=43&issue=2&spage=301 DB - PRIME DP - Unbound Medicine ER -