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The fabrication of protein chip based on surface plasmon resonance for detection of pathogens.
Biosens Bioelectron. 2005 Mar 15; 20(9):1847-50.BB

Abstract

Protein chip based on surface plasmon resonance (SPR) was developed for detection of pathogens existing in contaminated environment, such as Escherichia coli O157:H7, Salmonella typhimurium, Legionella pneumophila, and Yersinia enterocolitica. Protein G was immobilized to endow the orientation of antibody molecules on the SPR surface. The pathogen binding of the protein chip was investigated by SPR spectroscopy. Consequently, it was found that the four kinds of pathogen could be selectively detected by using SPR-based protein chip.

Authors+Show Affiliations

Oh BK
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea.
Lee W
No affiliation info available
Chun BS
No affiliation info available
Bae YM
No affiliation info available
Lee WH
No affiliation info available
Choi JW
No affiliation info available

MeSH

BacteriaBiocompatible MaterialsBiosensing TechniquesEquipment DesignEquipment Failure AnalysisImmunoassayMaterials TestingNerve Tissue ProteinsProtein Array AnalysisSurface Plasmon Resonance

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15681203

Citation

Oh, Byung-Keun, et al. "The Fabrication of Protein Chip Based On Surface Plasmon Resonance for Detection of Pathogens." Biosensors & Bioelectronics, vol. 20, no. 9, 2005, pp. 1847-50.
Oh BK, Lee W, Chun BS, et al. The fabrication of protein chip based on surface plasmon resonance for detection of pathogens. Biosens Bioelectron. 2005;20(9):1847-50.
Oh, B. K., Lee, W., Chun, B. S., Bae, Y. M., Lee, W. H., & Choi, J. W. (2005). The fabrication of protein chip based on surface plasmon resonance for detection of pathogens. Biosensors & Bioelectronics, 20(9), 1847-50.
Oh BK, et al. The Fabrication of Protein Chip Based On Surface Plasmon Resonance for Detection of Pathogens. Biosens Bioelectron. 2005 Mar 15;20(9):1847-50. PubMed PMID: 15681203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The fabrication of protein chip based on surface plasmon resonance for detection of pathogens. AU - Oh,Byung-Keun, AU - Lee,Woochang, AU - Chun,Bum Suk, AU - Bae,Young Min, AU - Lee,Won Hong, AU - Choi,Jeong-Woo, PY - 2003/12/22/received PY - 2004/05/05/revised PY - 2004/05/05/accepted PY - 2005/2/1/pubmed PY - 2005/6/25/medline PY - 2005/2/1/entrez SP - 1847 EP - 50 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 20 IS - 9 N2 - Protein chip based on surface plasmon resonance (SPR) was developed for detection of pathogens existing in contaminated environment, such as Escherichia coli O157:H7, Salmonella typhimurium, Legionella pneumophila, and Yersinia enterocolitica. Protein G was immobilized to endow the orientation of antibody molecules on the SPR surface. The pathogen binding of the protein chip was investigated by SPR spectroscopy. Consequently, it was found that the four kinds of pathogen could be selectively detected by using SPR-based protein chip. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/15681203/The_fabrication_of_protein_chip_based_on_surface_plasmon_resonance_for_detection_of_pathogens_ DB - PRIME DP - Unbound Medicine ER -