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Effects of glutamine-containing total parenteral nutrition on phagocytic activity and anabolic hormone response in rats undergoing gastrectomy.
World J Gastroenterol. 2005 Feb 14; 11(6):817-22.WJ

Abstract

AIM

To investigate the effect of glutamine (Gln)-containing parenteral nutrition on phagocytic activity and to elucidate the possible roles of Gln in the secretion of anabolic hormones and nitrogen balance in rats undergoing a gastrectomy.

METHODS

Rats with an internal jugular catheter were divided into 2 experimental groups and received total parenteral nutrition (TPN). The TPN solutions were isonitrogenous and identical in nutrient compositions except for differences in amino acid content. One group received conventional TPN (control), and in the other group, 25% of the total amino acid nitrogen was replaced with Gln. After receiving TPN for 3 d, one-third of the rats in each experimental group were sacrificed as the baseline group. The remaining rats underwent a partial gastrectomy and were killed 1 and 3 d, respectively, after surgery. Plasma, peritoneal lavage fluid (PLF), and urine samples were collected for further analysis.

RESULTS

The Gln group had fewer nitrogen losses 1 and 2 d after surgery (d1, 16.6+/-242.5 vs -233.4+/-205.9 mg/d, d2, 31.8+/-238.8 vs -253.4+/-184.6 mg/d, P<0.05). There were no differences in plasma growth hormone (GH) and insulin-like growth factor-1 levels between the 2 groups before or after surgery. The phagocytic activity of peritoneal macrophages was higher in the Gln group than in the control group 1 d after surgery (A 1185+/-931 vs 323+/-201, P<0.05). There were no differences in the phagocytic activities of blood polymorphonuclear neutrophils between the 2 groups at the baseline or on the postoperative days. No significant differences in interleukin-1beta or interleukin-6 concentrations in PLF were observed between the 2 groups. However, tumor necrosis factor-alpha level in PLF was significantly lower in the Gln group than in the control group on postoperative d 3.

CONCLUSION

TPN supplemented with Gln can improve the nitrogen balance, and enhance macrophage phagocytic activity at the site of injury. However, Gln supplementation has no effect on phagocytic cell activity in the systemic circulation, GH and insulin-like growth factor-1 might not be responsible for attenuating nitrogen losses in rats with a partial gastrectomy.

Authors+Show Affiliations

Institute of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan 110, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15682473

Citation

Lee, Chen-Hsien, et al. "Effects of Glutamine-containing Total Parenteral Nutrition On Phagocytic Activity and Anabolic Hormone Response in Rats Undergoing Gastrectomy." World Journal of Gastroenterology, vol. 11, no. 6, 2005, pp. 817-22.
Lee CH, Chiu WC, Chen SC, et al. Effects of glutamine-containing total parenteral nutrition on phagocytic activity and anabolic hormone response in rats undergoing gastrectomy. World J Gastroenterol. 2005;11(6):817-22.
Lee, C. H., Chiu, W. C., Chen, S. C., Wu, C. H., & Yeh, S. L. (2005). Effects of glutamine-containing total parenteral nutrition on phagocytic activity and anabolic hormone response in rats undergoing gastrectomy. World Journal of Gastroenterology, 11(6), 817-22.
Lee CH, et al. Effects of Glutamine-containing Total Parenteral Nutrition On Phagocytic Activity and Anabolic Hormone Response in Rats Undergoing Gastrectomy. World J Gastroenterol. 2005 Feb 14;11(6):817-22. PubMed PMID: 15682473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of glutamine-containing total parenteral nutrition on phagocytic activity and anabolic hormone response in rats undergoing gastrectomy. AU - Lee,Chen-Hsien, AU - Chiu,Wan-Chun, AU - Chen,Soul-Chin, AU - Wu,Chih-Hsiung, AU - Yeh,Sung-Ling, PY - 2005/2/1/pubmed PY - 2005/3/17/medline PY - 2005/2/1/entrez SP - 817 EP - 22 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 11 IS - 6 N2 - AIM: To investigate the effect of glutamine (Gln)-containing parenteral nutrition on phagocytic activity and to elucidate the possible roles of Gln in the secretion of anabolic hormones and nitrogen balance in rats undergoing a gastrectomy. METHODS: Rats with an internal jugular catheter were divided into 2 experimental groups and received total parenteral nutrition (TPN). The TPN solutions were isonitrogenous and identical in nutrient compositions except for differences in amino acid content. One group received conventional TPN (control), and in the other group, 25% of the total amino acid nitrogen was replaced with Gln. After receiving TPN for 3 d, one-third of the rats in each experimental group were sacrificed as the baseline group. The remaining rats underwent a partial gastrectomy and were killed 1 and 3 d, respectively, after surgery. Plasma, peritoneal lavage fluid (PLF), and urine samples were collected for further analysis. RESULTS: The Gln group had fewer nitrogen losses 1 and 2 d after surgery (d1, 16.6+/-242.5 vs -233.4+/-205.9 mg/d, d2, 31.8+/-238.8 vs -253.4+/-184.6 mg/d, P<0.05). There were no differences in plasma growth hormone (GH) and insulin-like growth factor-1 levels between the 2 groups before or after surgery. The phagocytic activity of peritoneal macrophages was higher in the Gln group than in the control group 1 d after surgery (A 1185+/-931 vs 323+/-201, P<0.05). There were no differences in the phagocytic activities of blood polymorphonuclear neutrophils between the 2 groups at the baseline or on the postoperative days. No significant differences in interleukin-1beta or interleukin-6 concentrations in PLF were observed between the 2 groups. However, tumor necrosis factor-alpha level in PLF was significantly lower in the Gln group than in the control group on postoperative d 3. CONCLUSION: TPN supplemented with Gln can improve the nitrogen balance, and enhance macrophage phagocytic activity at the site of injury. However, Gln supplementation has no effect on phagocytic cell activity in the systemic circulation, GH and insulin-like growth factor-1 might not be responsible for attenuating nitrogen losses in rats with a partial gastrectomy. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/15682473/Effects_of_glutamine_containing_total_parenteral_nutrition_on_phagocytic_activity_and_anabolic_hormone_response_in_rats_undergoing_gastrectomy_ L2 - https://www.wjgnet.com/1007-9327/full/v11/i6/817.htm DB - PRIME DP - Unbound Medicine ER -