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Comparison of probiotics and lactulose in the treatment of minimal hepatic encephalopathy in rats.

Abstract

AIM

To compare the efficacy of probiotic preparation Golden Bifid and lactulose on rat experimental model of minimal hepatic encephalopathy (MHE) induced by thioactamide (TAA).

METHODS

MHE was induced by intraperitoneal injection of TAA (200 mg/kg) every 24 h for two consecutive days. Thirty-six male MHE models were then randomly divided into 3 groups: TAA group (n = 12) received tap water ad libitum only; lactulose group (n = 12) and probiotics group (n = 12) were gavaged, respectively with 8 mL/kg of lactulose and 1.5 g/kg of probiotic preparation Golden Bifid (highly concentrated combination of probiotic) dissolved in 2 mL of normal saline, once a day for 8 d (from the 5(th) d before the experiment to the 3(rd) d of the experiment). The latency of brainstem auditory evoked potentials (BAEP) I was used as an objective index of MHE. The incidence of MHE, the level of serum endotoxin, ammonia, liver function and histological grade of hepatic injury of rats were examined individually.

RESULTS

There were no overt HE and rat deaths in 3 groups. The incidence of MHE, the levels of blood ammonia and endotoxin in TAA group, which were 83.3% (10/12), 168.33+/-15.44 mg/dL and 0.36+/-0.04 EU/mL, respectively, were significantly higher than those in lactulose group, which were 33.3% (4/12), 110.25+/-7.39 mg/dL and 0.19+/-0.02 EU/mL, and probiotics group, which were 33.3% (4/12), 108.58+/-10.24 mg/dL and 0.13+/-0.03 EU/mL respectively (P<0.001). It showed that either probiotics or lactulose could significantly lower the level of hyperammonemia and hyper-endotoxemia, lighten centrolobular necrotic areas as well as inflammatory reaction in the liver of rats, normalize the latency of BAEP, and decrease the incidence of MHE. However, no significant differences were observed between these two groups (P>0.05).

CONCLUSION

Probiotic compound Golden Bifid is at least as useful as lactulose for the prevention and treatment of MHE. Probiotic therapy may be a safe, natural, well-tolerated therapy appropriate for the long-term treatment of MHE.

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  • Authors+Show Affiliations

    ,

    Department of Digestive Diseases, Affiliated First People's Municipal Hospital, Guangzhou Medical College, Guangdong, China. gzjialin@hotmail.com

    Source

    World journal of gastroenterology 11:6 2005 Feb 14 pg 908-11

    MeSH

    Ammonia
    Animals
    Endotoxins
    Gastrointestinal Agents
    Hepatic Encephalopathy
    Incidence
    Lactulose
    Liver
    Liver Function Tests
    Male
    Probiotics
    Rats
    Rats, Sprague-Dawley
    Severity of Illness Index
    Thioacetamide

    Pub Type(s)

    Comparative Study
    Journal Article

    Language

    eng

    PubMed ID

    15682492

    Citation

    Jia, Lin, and Mei-Hua Zhang. "Comparison of Probiotics and Lactulose in the Treatment of Minimal Hepatic Encephalopathy in Rats." World Journal of Gastroenterology, vol. 11, no. 6, 2005, pp. 908-11.
    Jia L, Zhang MH. Comparison of probiotics and lactulose in the treatment of minimal hepatic encephalopathy in rats. World J Gastroenterol. 2005;11(6):908-11.
    Jia, L., & Zhang, M. H. (2005). Comparison of probiotics and lactulose in the treatment of minimal hepatic encephalopathy in rats. World Journal of Gastroenterology, 11(6), pp. 908-11.
    Jia L, Zhang MH. Comparison of Probiotics and Lactulose in the Treatment of Minimal Hepatic Encephalopathy in Rats. World J Gastroenterol. 2005 Feb 14;11(6):908-11. PubMed PMID: 15682492.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Comparison of probiotics and lactulose in the treatment of minimal hepatic encephalopathy in rats. AU - Jia,Lin, AU - Zhang,Mei-Hua, PY - 2005/2/1/pubmed PY - 2005/3/17/medline PY - 2005/2/1/entrez SP - 908 EP - 11 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 11 IS - 6 N2 - AIM: To compare the efficacy of probiotic preparation Golden Bifid and lactulose on rat experimental model of minimal hepatic encephalopathy (MHE) induced by thioactamide (TAA). METHODS: MHE was induced by intraperitoneal injection of TAA (200 mg/kg) every 24 h for two consecutive days. Thirty-six male MHE models were then randomly divided into 3 groups: TAA group (n = 12) received tap water ad libitum only; lactulose group (n = 12) and probiotics group (n = 12) were gavaged, respectively with 8 mL/kg of lactulose and 1.5 g/kg of probiotic preparation Golden Bifid (highly concentrated combination of probiotic) dissolved in 2 mL of normal saline, once a day for 8 d (from the 5(th) d before the experiment to the 3(rd) d of the experiment). The latency of brainstem auditory evoked potentials (BAEP) I was used as an objective index of MHE. The incidence of MHE, the level of serum endotoxin, ammonia, liver function and histological grade of hepatic injury of rats were examined individually. RESULTS: There were no overt HE and rat deaths in 3 groups. The incidence of MHE, the levels of blood ammonia and endotoxin in TAA group, which were 83.3% (10/12), 168.33+/-15.44 mg/dL and 0.36+/-0.04 EU/mL, respectively, were significantly higher than those in lactulose group, which were 33.3% (4/12), 110.25+/-7.39 mg/dL and 0.19+/-0.02 EU/mL, and probiotics group, which were 33.3% (4/12), 108.58+/-10.24 mg/dL and 0.13+/-0.03 EU/mL respectively (P<0.001). It showed that either probiotics or lactulose could significantly lower the level of hyperammonemia and hyper-endotoxemia, lighten centrolobular necrotic areas as well as inflammatory reaction in the liver of rats, normalize the latency of BAEP, and decrease the incidence of MHE. However, no significant differences were observed between these two groups (P>0.05). CONCLUSION: Probiotic compound Golden Bifid is at least as useful as lactulose for the prevention and treatment of MHE. Probiotic therapy may be a safe, natural, well-tolerated therapy appropriate for the long-term treatment of MHE. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/15682492/Comparison_of_probiotics_and_lactulose_in_the_treatment_of_minimal_hepatic_encephalopathy_in_rats_ L2 - http://www.wjgnet.com/1007-9327/full/v11/i6/908.htm DB - PRIME DP - Unbound Medicine ER -