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Molecular characterization of cyanobacterial diversity in a shallow eutrophic lake.
Environ Microbiol. 2005 Mar; 7(3):365-77.EM

Abstract

We have studied the diversity of pelagic cyanobacteria in Lake Loosdrecht, The Netherlands, through recovery and analysis of small subunit ribosomal RNA gene sequences from lake samples and cyanobacterial isolates. We used an adapted protocol for specific amplification of cyanobacterial rDNA for denaturing gradient gel electrophoresis (DGGE) analysis. This protocol enabled direct comparison of cyanobacterial community profiles with overall bacterial profiles. The theoretical amplification specificity of the primers was supported by sequence analysis of DNA from excised DGGE bands. Sequences recovered from these bands, in addition to sequences obtained by polymerase chain reaction (PCR) and cloning from lake DNA as well as from cyanobacterial isolates from the lake, revealed a diverse consortium of cyanobacteria, among which are representatives of the genera Aphanizomenon, Planktothrix, Microcystis and Synechococcus. One numerically important and persistent cyanobacterium in the lake, Prochlorothrix hollandica, appeared to co-occur with an unknown but related species. However, the lake is dominated by filamentous species that originally have been termed 'Oscillatoria limnetica-like'. We show that this is a group of several related cyanobacteria, co-occurring in the lake, which belong to the Limnothrix/Pseudanabaena group. The available variation among the coexisting strains of this group can explain the persistent dominance of the group under severe viral pressure.

Authors+Show Affiliations

Centre for Limnology, NIOO-KNAW Netherlands Institute of Ecology, Rijksstraatweg 6, 3631 AC Nieuwersluis, The Netherlands. g.zwart@nioo.knaw.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15683397

Citation

Zwart, Gabriel, et al. "Molecular Characterization of Cyanobacterial Diversity in a Shallow Eutrophic Lake." Environmental Microbiology, vol. 7, no. 3, 2005, pp. 365-77.
Zwart G, Kamst-van Agterveld MP, van der Werff-Staverman I, et al. Molecular characterization of cyanobacterial diversity in a shallow eutrophic lake. Environ Microbiol. 2005;7(3):365-77.
Zwart, G., Kamst-van Agterveld, M. P., van der Werff-Staverman, I., Hagen, F., Hoogveld, H. L., & Gons, H. J. (2005). Molecular characterization of cyanobacterial diversity in a shallow eutrophic lake. Environmental Microbiology, 7(3), 365-77.
Zwart G, et al. Molecular Characterization of Cyanobacterial Diversity in a Shallow Eutrophic Lake. Environ Microbiol. 2005;7(3):365-77. PubMed PMID: 15683397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of cyanobacterial diversity in a shallow eutrophic lake. AU - Zwart,Gabriel, AU - Kamst-van Agterveld,Miranda P, AU - van der Werff-Staverman,Irene, AU - Hagen,Ferry, AU - Hoogveld,Hans L, AU - Gons,Herman J, PY - 2005/2/3/pubmed PY - 2005/4/26/medline PY - 2005/2/3/entrez SP - 365 EP - 77 JF - Environmental microbiology JO - Environ Microbiol VL - 7 IS - 3 N2 - We have studied the diversity of pelagic cyanobacteria in Lake Loosdrecht, The Netherlands, through recovery and analysis of small subunit ribosomal RNA gene sequences from lake samples and cyanobacterial isolates. We used an adapted protocol for specific amplification of cyanobacterial rDNA for denaturing gradient gel electrophoresis (DGGE) analysis. This protocol enabled direct comparison of cyanobacterial community profiles with overall bacterial profiles. The theoretical amplification specificity of the primers was supported by sequence analysis of DNA from excised DGGE bands. Sequences recovered from these bands, in addition to sequences obtained by polymerase chain reaction (PCR) and cloning from lake DNA as well as from cyanobacterial isolates from the lake, revealed a diverse consortium of cyanobacteria, among which are representatives of the genera Aphanizomenon, Planktothrix, Microcystis and Synechococcus. One numerically important and persistent cyanobacterium in the lake, Prochlorothrix hollandica, appeared to co-occur with an unknown but related species. However, the lake is dominated by filamentous species that originally have been termed 'Oscillatoria limnetica-like'. We show that this is a group of several related cyanobacteria, co-occurring in the lake, which belong to the Limnothrix/Pseudanabaena group. The available variation among the coexisting strains of this group can explain the persistent dominance of the group under severe viral pressure. SN - 1462-2912 UR - https://www.unboundmedicine.com/medline/citation/15683397/Molecular_characterization_of_cyanobacterial_diversity_in_a_shallow_eutrophic_lake_ L2 - https://doi.org/10.1111/j.1462-2920.2005.00715.x DB - PRIME DP - Unbound Medicine ER -