Tags

Type your tag names separated by a space and hit enter

A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium.
BMC Infect Dis. 2005 Feb 01; 5:6.BI

Abstract

BACKGROUND

Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium.

METHODS

Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay.

RESULTS

Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004.

CONCLUSIONS

Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating.

Authors+Show Affiliations

Laboratory of Clinical & Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research, University of Leuven, Belgium. elien.moes@uz.kuleuven.ac.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15686594

Citation

Moës, Elien, et al. "A Novel Pancoronavirus RT-PCR Assay: Frequent Detection of Human Coronavirus NL63 in Children Hospitalized With Respiratory Tract Infections in Belgium." BMC Infectious Diseases, vol. 5, 2005, p. 6.
Moës E, Vijgen L, Keyaerts E, et al. A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium. BMC Infect Dis. 2005;5:6.
Moës, E., Vijgen, L., Keyaerts, E., Zlateva, K., Li, S., Maes, P., Pyrc, K., Berkhout, B., van der Hoek, L., & Van Ranst, M. (2005). A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium. BMC Infectious Diseases, 5, 6.
Moës E, et al. A Novel Pancoronavirus RT-PCR Assay: Frequent Detection of Human Coronavirus NL63 in Children Hospitalized With Respiratory Tract Infections in Belgium. BMC Infect Dis. 2005 Feb 1;5:6. PubMed PMID: 15686594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium. AU - Moës,Elien, AU - Vijgen,Leen, AU - Keyaerts,Els, AU - Zlateva,Kalina, AU - Li,Sandra, AU - Maes,Piet, AU - Pyrc,Krzysztof, AU - Berkhout,Ben, AU - van der Hoek,Lia, AU - Van Ranst,Marc, Y1 - 2005/02/01/ PY - 2004/10/13/received PY - 2005/02/01/accepted PY - 2005/2/3/pubmed PY - 2006/4/21/medline PY - 2005/2/3/entrez SP - 6 EP - 6 JF - BMC infectious diseases JO - BMC Infect Dis VL - 5 N2 - BACKGROUND: Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium. METHODS: Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay. RESULTS: Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004. CONCLUSIONS: Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating. SN - 1471-2334 UR - https://www.unboundmedicine.com/medline/citation/15686594/A_novel_pancoronavirus_RT_PCR_assay:_frequent_detection_of_human_coronavirus_NL63_in_children_hospitalized_with_respiratory_tract_infections_in_Belgium_ L2 - https://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-5-6 DB - PRIME DP - Unbound Medicine ER -