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Binding of DNA to alpha/beta-type small, acid-soluble proteins from spores of Bacillus or Clostridium species prevents formation of cytosine dimers, cytosine-thymine dimers, and bipyrimidine photoadducts after UV irradiation.
J Bacteriol. 1992 May; 174(9):2874-80.JB

Abstract

Small, acid-soluble proteins (SASP) of the alpha/beta-type from spores of Bacillus and Clostridium species bind to DNA; this binding prevents formation of cyclobutane-type thymine dimers upon UV irradiation, but promotes formation of the spore photoproduct, an adduct between adjacent thymine residues. alpha/beta-Type SASP also bound to poly(dG).poly(dC) and poly(dA-dG).poly(dC-dT). While UV irradiation of poly(dG).poly(dC) produced cyclobutane-type cytosine dimers as well as fluorescent bipyrimidine adducts, the yields of both types of photoproduct were greatly reduced upon irradiation of alpha/beta-type SASP-poly(dG).poly(dC) complexes. UV irradiation of poly(dA-dG).poly(dC-dT) produced a significant amount of a cyclobutane dimer between cytosine and thymine, as well as a 6-4 bipyrimidine adduct. Again, binding of alpha/beta-type SASP to poly(dA-dG).poly(dC-dT) greatly reduced formation of these two photoproducts, although formation of the cytosine-thymine analog of the spore photoproduct was not observed. These data provide further evidence for the dramatic change in DNA structure and photoreactivity which takes place on binding of alpha/beta-type SASP and suggest that binding of these proteins to DNA in vivo prevents formation of most deleterious photoproducts upon UV irradiation.

Authors+Show Affiliations

Department of Biochemistry, University of Connecticut Health Center, Farmington 06030.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1569018

Citation

Fairhead, H, and P Setlow. "Binding of DNA to Alpha/beta-type Small, Acid-soluble Proteins From Spores of Bacillus or Clostridium Species Prevents Formation of Cytosine Dimers, Cytosine-thymine Dimers, and Bipyrimidine Photoadducts After UV Irradiation." Journal of Bacteriology, vol. 174, no. 9, 1992, pp. 2874-80.
Fairhead H, Setlow P. Binding of DNA to alpha/beta-type small, acid-soluble proteins from spores of Bacillus or Clostridium species prevents formation of cytosine dimers, cytosine-thymine dimers, and bipyrimidine photoadducts after UV irradiation. J Bacteriol. 1992;174(9):2874-80.
Fairhead, H., & Setlow, P. (1992). Binding of DNA to alpha/beta-type small, acid-soluble proteins from spores of Bacillus or Clostridium species prevents formation of cytosine dimers, cytosine-thymine dimers, and bipyrimidine photoadducts after UV irradiation. Journal of Bacteriology, 174(9), 2874-80.
Fairhead H, Setlow P. Binding of DNA to Alpha/beta-type Small, Acid-soluble Proteins From Spores of Bacillus or Clostridium Species Prevents Formation of Cytosine Dimers, Cytosine-thymine Dimers, and Bipyrimidine Photoadducts After UV Irradiation. J Bacteriol. 1992;174(9):2874-80. PubMed PMID: 1569018.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Binding of DNA to alpha/beta-type small, acid-soluble proteins from spores of Bacillus or Clostridium species prevents formation of cytosine dimers, cytosine-thymine dimers, and bipyrimidine photoadducts after UV irradiation. AU - Fairhead,H, AU - Setlow,P, PY - 1992/5/1/pubmed PY - 1992/5/1/medline PY - 1992/5/1/entrez SP - 2874 EP - 80 JF - Journal of bacteriology JO - J. Bacteriol. VL - 174 IS - 9 N2 - Small, acid-soluble proteins (SASP) of the alpha/beta-type from spores of Bacillus and Clostridium species bind to DNA; this binding prevents formation of cyclobutane-type thymine dimers upon UV irradiation, but promotes formation of the spore photoproduct, an adduct between adjacent thymine residues. alpha/beta-Type SASP also bound to poly(dG).poly(dC) and poly(dA-dG).poly(dC-dT). While UV irradiation of poly(dG).poly(dC) produced cyclobutane-type cytosine dimers as well as fluorescent bipyrimidine adducts, the yields of both types of photoproduct were greatly reduced upon irradiation of alpha/beta-type SASP-poly(dG).poly(dC) complexes. UV irradiation of poly(dA-dG).poly(dC-dT) produced a significant amount of a cyclobutane dimer between cytosine and thymine, as well as a 6-4 bipyrimidine adduct. Again, binding of alpha/beta-type SASP to poly(dA-dG).poly(dC-dT) greatly reduced formation of these two photoproducts, although formation of the cytosine-thymine analog of the spore photoproduct was not observed. These data provide further evidence for the dramatic change in DNA structure and photoreactivity which takes place on binding of alpha/beta-type SASP and suggest that binding of these proteins to DNA in vivo prevents formation of most deleterious photoproducts upon UV irradiation. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/1569018/Binding_of_DNA_to_alpha/beta_type_small_acid_soluble_proteins_from_spores_of_Bacillus_or_Clostridium_species_prevents_formation_of_cytosine_dimers_cytosine_thymine_dimers_and_bipyrimidine_photoadducts_after_UV_irradiation_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=1569018 DB - PRIME DP - Unbound Medicine ER -