Tags

Type your tag names separated by a space and hit enter

Telomerase activity and hTERT mRNA expression in body fluids.
Diagn Cytopathol. 2005 Mar; 32(3):167-70.DC

Abstract

Telomerase is a ribonucleoprotein enzyme associated with cellular immortality, and its activity is detectable in most human tumors and immortalized cells. In the present study, we examined telomerase activities and gene expression of human telomerase reverse transcriptase (hTERT) to detect malignant cells in body fluid. Twenty-eight cytological fluids were obtained from 28 patients, including 12 patients with benign cases and 16 patients with malignant tumors (14 ascitic fluids, 12 pleural fluids, 1 peritoneal washing, and 1 pericardial fluid). Telomerase activity was measured using a quantitative telomeric repeat amplification protocol (TRAP), and the hTERT messenger RNA (mRNA) expression was detected by reverse-transcription polymerase chain reaction (RT-PCR). Of the 16 malignant cases, telomerase activity was detected in 8 cases, and 10 cases involved expression of (hTERT) (mRNA). Neither telomerase activity nor hTERT mRNA expression was detected in benign cases. The telomerase activity and mRNA expression exhibited sensitivities of 50 and 62.5%, respectively, and both methods showed a specificity of 100%. The hTERT mRNA expression is a more sensitive marker than telomerase activity. Our results suggest that measurement of mRNA expression of hTERT in body fluid is useful as an adjunctive tool for cytological diagnosis.

Authors+Show Affiliations

Laboratory of Pathology, Department of Medical Technology, Kagawa Prefectural College of Health Sciences, Kagawa, Japan. yamamoto_y@chs.pref.kagawa.jpNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15690332

Citation

Yamamoto, Yasuko, and Eiichiro Hirakawa. "Telomerase Activity and hTERT mRNA Expression in Body Fluids." Diagnostic Cytopathology, vol. 32, no. 3, 2005, pp. 167-70.
Yamamoto Y, Hirakawa E. Telomerase activity and hTERT mRNA expression in body fluids. Diagn Cytopathol. 2005;32(3):167-70.
Yamamoto, Y., & Hirakawa, E. (2005). Telomerase activity and hTERT mRNA expression in body fluids. Diagnostic Cytopathology, 32(3), 167-70.
Yamamoto Y, Hirakawa E. Telomerase Activity and hTERT mRNA Expression in Body Fluids. Diagn Cytopathol. 2005;32(3):167-70. PubMed PMID: 15690332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Telomerase activity and hTERT mRNA expression in body fluids. AU - Yamamoto,Yasuko, AU - Hirakawa,Eiichiro, PY - 2005/2/4/pubmed PY - 2005/6/17/medline PY - 2005/2/4/entrez SP - 167 EP - 70 JF - Diagnostic cytopathology JO - Diagn Cytopathol VL - 32 IS - 3 N2 - Telomerase is a ribonucleoprotein enzyme associated with cellular immortality, and its activity is detectable in most human tumors and immortalized cells. In the present study, we examined telomerase activities and gene expression of human telomerase reverse transcriptase (hTERT) to detect malignant cells in body fluid. Twenty-eight cytological fluids were obtained from 28 patients, including 12 patients with benign cases and 16 patients with malignant tumors (14 ascitic fluids, 12 pleural fluids, 1 peritoneal washing, and 1 pericardial fluid). Telomerase activity was measured using a quantitative telomeric repeat amplification protocol (TRAP), and the hTERT messenger RNA (mRNA) expression was detected by reverse-transcription polymerase chain reaction (RT-PCR). Of the 16 malignant cases, telomerase activity was detected in 8 cases, and 10 cases involved expression of (hTERT) (mRNA). Neither telomerase activity nor hTERT mRNA expression was detected in benign cases. The telomerase activity and mRNA expression exhibited sensitivities of 50 and 62.5%, respectively, and both methods showed a specificity of 100%. The hTERT mRNA expression is a more sensitive marker than telomerase activity. Our results suggest that measurement of mRNA expression of hTERT in body fluid is useful as an adjunctive tool for cytological diagnosis. SN - 8755-1039 UR - https://www.unboundmedicine.com/medline/citation/15690332/Telomerase_activity_and_hTERT_mRNA_expression_in_body_fluids_ L2 - https://doi.org/10.1002/dc.20204 DB - PRIME DP - Unbound Medicine ER -