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Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection.
Electrophoresis. 2005 Feb; 26(3):593-9.E

Abstract

A capillary electrophoresis (CE) method with laser-induced fluorescence (LIF) detection was developed for the separation and detection of carnosine-related peptides (carnosine, anserine, and homocarnosine). A sensitive and fluorogenic regent, 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde (CBQCA) was selected as a precapillary labeling reagent for imidazole dipeptides to form isoindole derivatives. The optimized molar ratio between CBQCA and peptide was found to be 75:1, and 50 mmol/L borate buffer (pH 9.2) was used for the derivatization in order to achieve good efficiency. Three imidazole dipeptides were baseline-separated within 20 min by using 112 mmol/L sodium borate (pH 10.4-10.8) as running buffer. Concentration detection limits (signal-to-noise ratios) for carnosine, anserine, and homocarnosine were 4.73, 4.37, and 3.94 nmol/L, respectively. This method has been applied to the analysis of human cerebrospinal fluid (CSF) and meat dry powder of pig and sheep. Recoveries were in the range of 82.9-104.8% for homocarnosine in CSF. For carnosine and anserine, the recoveries are 98.3% and 80.2% in meat dry powder of pig and 111.2% and 112.8% in meat dry powder of sheep, respectively.

Authors+Show Affiliations

Huang Y
Department of Chemistry, Fuzhou University, Fujian, China.
Duan J
No affiliation info available
Chen H
No affiliation info available
Chen M
No affiliation info available
Chen G
No affiliation info available

MeSH

AdolescentAdultAnimalsAnserineBenzoatesCarnosineCerebrospinal FluidElectrophoresis, CapillaryHumansLasersMeatMiddle AgedMusclesPeptidesQuinolinesSheepSpectrometry, FluorescenceSus scrofa

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15690432

Citation

Huang, Ying, et al. "Separation and Determination of Carnosine-related Peptides Using Capillary Electrophoresis With Laser-induced Fluorescence Detection." Electrophoresis, vol. 26, no. 3, 2005, pp. 593-9.
Huang Y, Duan J, Chen H, et al. Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis. 2005;26(3):593-9.
Huang, Y., Duan, J., Chen, H., Chen, M., & Chen, G. (2005). Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis, 26(3), 593-9.
Huang Y, et al. Separation and Determination of Carnosine-related Peptides Using Capillary Electrophoresis With Laser-induced Fluorescence Detection. Electrophoresis. 2005;26(3):593-9. PubMed PMID: 15690432.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection. AU - Huang,Ying, AU - Duan,Jianping, AU - Chen,Hongqing, AU - Chen,Min, AU - Chen,Guonan, PY - 2005/2/4/pubmed PY - 2005/6/10/medline PY - 2005/2/4/entrez SP - 593 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 26 IS - 3 N2 - A capillary electrophoresis (CE) method with laser-induced fluorescence (LIF) detection was developed for the separation and detection of carnosine-related peptides (carnosine, anserine, and homocarnosine). A sensitive and fluorogenic regent, 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde (CBQCA) was selected as a precapillary labeling reagent for imidazole dipeptides to form isoindole derivatives. The optimized molar ratio between CBQCA and peptide was found to be 75:1, and 50 mmol/L borate buffer (pH 9.2) was used for the derivatization in order to achieve good efficiency. Three imidazole dipeptides were baseline-separated within 20 min by using 112 mmol/L sodium borate (pH 10.4-10.8) as running buffer. Concentration detection limits (signal-to-noise ratios) for carnosine, anserine, and homocarnosine were 4.73, 4.37, and 3.94 nmol/L, respectively. This method has been applied to the analysis of human cerebrospinal fluid (CSF) and meat dry powder of pig and sheep. Recoveries were in the range of 82.9-104.8% for homocarnosine in CSF. For carnosine and anserine, the recoveries are 98.3% and 80.2% in meat dry powder of pig and 111.2% and 112.8% in meat dry powder of sheep, respectively. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15690432/Separation_and_determination_of_carnosine_related_peptides_using_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -