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Characterization of Orientia tsutsugamushi strains isolated in Shandong Province, China by immunofluorescence and restriction fragment length polymorphism (RFLP) analyses.
Southeast Asian J Trop Med Public Health. 2004 Jun; 35(2):353-7.SA

Abstract

In order to identify the characteristics of the Sta56 gene of the 23 isolates of Orientia (O.) tsutsugamushi isolated in Shandong Province, indirect immunofluorescence assay (IFA) was used to identify the gene type of 23 strains O. tsutsugamushi isolated from scrub typhus patients, chigger mites, and rodents. Restriction fragment length polymorphism (RFLP) analysis was also used to analyze the restriction profiles of the Sta56 gene PCR amplification products of the 23 isolated strains of the O. tsutsugamushi; the results were compared with those acquired by nested PCR. By IFA, 21 of the 23 isolates belonged to the Gilliam type, and 2 to the Karp type. Using RFLP analysis, 21 strains had similar restriction profiles to the Japan Kawasaki strain, but they had no restriction site Hha I, and thus had some difference in gene sequence compared with the Japan Kawasaki strain. The other 2 strains had similar restriction profiles to Karp. These results were identical to that acquired by nested-PCR. In Shandong Province, the gene types of epidemic O. tsutsugamushi strains were similar to the Japan Kawasaki type, but had some differences in gene sequence. In addition, Karp also existed.

Authors+Show Affiliations

Liu YX
School of Public Health, Shandong University, Jinan, PR China.
Zhao ZT
No affiliation info available
Gao Y
No affiliation info available
Jia CQ
No affiliation info available
Zhang JL
No affiliation info available
Yang ZQ
No affiliation info available
Wang SM
No affiliation info available
Jiang BF
No affiliation info available

MeSH

AnimalsArthropod VectorsBites and StingsChinaFluoroimmunoassayMiceOrientia tsutsugamushiPolymorphism, Restriction Fragment LengthScrub TyphusTrombiculidae

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15691135

Citation

Liu, Yun-xi, et al. "Characterization of Orientia Tsutsugamushi Strains Isolated in Shandong Province, China By Immunofluorescence and Restriction Fragment Length Polymorphism (RFLP) Analyses." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 35, no. 2, 2004, pp. 353-7.
Liu YX, Zhao ZT, Gao Y, et al. Characterization of Orientia tsutsugamushi strains isolated in Shandong Province, China by immunofluorescence and restriction fragment length polymorphism (RFLP) analyses. Southeast Asian J Trop Med Public Health. 2004;35(2):353-7.
Liu, Y. X., Zhao, Z. T., Gao, Y., Jia, C. Q., Zhang, J. L., Yang, Z. Q., Wang, S. M., & Jiang, B. F. (2004). Characterization of Orientia tsutsugamushi strains isolated in Shandong Province, China by immunofluorescence and restriction fragment length polymorphism (RFLP) analyses. The Southeast Asian Journal of Tropical Medicine and Public Health, 35(2), 353-7.
Liu YX, et al. Characterization of Orientia Tsutsugamushi Strains Isolated in Shandong Province, China By Immunofluorescence and Restriction Fragment Length Polymorphism (RFLP) Analyses. Southeast Asian J Trop Med Public Health. 2004;35(2):353-7. PubMed PMID: 15691135.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Orientia tsutsugamushi strains isolated in Shandong Province, China by immunofluorescence and restriction fragment length polymorphism (RFLP) analyses. AU - Liu,Yun-xi, AU - Zhao,Zhong-tang, AU - Gao,Yuan, AU - Jia,Chong-qi, AU - Zhang,Jing-lan, AU - Yang,Zhan-qing, AU - Wang,Shu-mei, AU - Jiang,Bao-fa, PY - 2005/2/5/pubmed PY - 2005/5/10/medline PY - 2005/2/5/entrez SP - 353 EP - 7 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 35 IS - 2 N2 - In order to identify the characteristics of the Sta56 gene of the 23 isolates of Orientia (O.) tsutsugamushi isolated in Shandong Province, indirect immunofluorescence assay (IFA) was used to identify the gene type of 23 strains O. tsutsugamushi isolated from scrub typhus patients, chigger mites, and rodents. Restriction fragment length polymorphism (RFLP) analysis was also used to analyze the restriction profiles of the Sta56 gene PCR amplification products of the 23 isolated strains of the O. tsutsugamushi; the results were compared with those acquired by nested PCR. By IFA, 21 of the 23 isolates belonged to the Gilliam type, and 2 to the Karp type. Using RFLP analysis, 21 strains had similar restriction profiles to the Japan Kawasaki strain, but they had no restriction site Hha I, and thus had some difference in gene sequence compared with the Japan Kawasaki strain. The other 2 strains had similar restriction profiles to Karp. These results were identical to that acquired by nested-PCR. In Shandong Province, the gene types of epidemic O. tsutsugamushi strains were similar to the Japan Kawasaki type, but had some differences in gene sequence. In addition, Karp also existed. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/15691135/Characterization_of_Orientia_tsutsugamushi_strains_isolated_in_Shandong_Province_China_by_immunofluorescence_and_restriction_fragment_length_polymorphism__RFLP__analyses_ DB - PRIME DP - Unbound Medicine ER -