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Metabolic engineering of a xylose-isomerase-expressing Saccharomyces cerevisiae strain for rapid anaerobic xylose fermentation.
FEMS Yeast Res. 2005 Feb; 5(4-5):399-409.FY

Abstract

After an extensive selection procedure, Saccharomyces cerevisiae strains that express the xylose isomerase gene from the fungus Piromyces sp. E2 can grow anaerobically on xylose with a mu(max) of 0.03 h(-1). In order to investigate whether reactions downstream of the isomerase control the rate of xylose consumption, we overexpressed structural genes for all enzymes involved in the conversion of xylulose to glycolytic intermediates, in a xylose-isomerase-expressing S. cerevisiae strain. The overexpressed enzymes were xylulokinase (EC 2.7.1.17), ribulose 5-phosphate isomerase (EC 5.3.1.6), ribulose 5-phosphate epimerase (EC 5.3.1.1), transketolase (EC 2.2.1.1) and transaldolase (EC 2.2.1.2). In addition, the GRE3 gene encoding aldose reductase was deleted to further minimise xylitol production. Surprisingly the resulting strain grew anaerobically on xylose in synthetic media with a mu(max) as high as 0.09 h(-1) without any non-defined mutagenesis or selection. During growth on xylose, xylulose formation was absent and xylitol production was negligible. The specific xylose consumption rate in anaerobic xylose cultures was 1.1 g xylose (g biomass)(-1) h(-1). Mixtures of glucose and xylose were sequentially but completely consumed by anaerobic batch cultures, with glucose as the preferred substrate.

Authors+Show Affiliations

Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15691745

Citation

Kuyper, Marko, et al. "Metabolic Engineering of a Xylose-isomerase-expressing Saccharomyces Cerevisiae Strain for Rapid Anaerobic Xylose Fermentation." FEMS Yeast Research, vol. 5, no. 4-5, 2005, pp. 399-409.
Kuyper M, Hartog MM, Toirkens MJ, et al. Metabolic engineering of a xylose-isomerase-expressing Saccharomyces cerevisiae strain for rapid anaerobic xylose fermentation. FEMS Yeast Res. 2005;5(4-5):399-409.
Kuyper, M., Hartog, M. M., Toirkens, M. J., Almering, M. J., Winkler, A. A., van Dijken, J. P., & Pronk, J. T. (2005). Metabolic engineering of a xylose-isomerase-expressing Saccharomyces cerevisiae strain for rapid anaerobic xylose fermentation. FEMS Yeast Research, 5(4-5), 399-409.
Kuyper M, et al. Metabolic Engineering of a Xylose-isomerase-expressing Saccharomyces Cerevisiae Strain for Rapid Anaerobic Xylose Fermentation. FEMS Yeast Res. 2005;5(4-5):399-409. PubMed PMID: 15691745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic engineering of a xylose-isomerase-expressing Saccharomyces cerevisiae strain for rapid anaerobic xylose fermentation. AU - Kuyper,Marko, AU - Hartog,Miranda M P, AU - Toirkens,Maurice J, AU - Almering,Marinka J H, AU - Winkler,Aaron A, AU - van Dijken,Johannes P, AU - Pronk,Jack T, PY - 2004/08/10/received PY - 2004/09/23/revised PY - 2004/09/24/accepted PY - 2005/2/5/pubmed PY - 2005/5/11/medline PY - 2005/2/5/entrez SP - 399 EP - 409 JF - FEMS yeast research JO - FEMS Yeast Res VL - 5 IS - 4-5 N2 - After an extensive selection procedure, Saccharomyces cerevisiae strains that express the xylose isomerase gene from the fungus Piromyces sp. E2 can grow anaerobically on xylose with a mu(max) of 0.03 h(-1). In order to investigate whether reactions downstream of the isomerase control the rate of xylose consumption, we overexpressed structural genes for all enzymes involved in the conversion of xylulose to glycolytic intermediates, in a xylose-isomerase-expressing S. cerevisiae strain. The overexpressed enzymes were xylulokinase (EC 2.7.1.17), ribulose 5-phosphate isomerase (EC 5.3.1.6), ribulose 5-phosphate epimerase (EC 5.3.1.1), transketolase (EC 2.2.1.1) and transaldolase (EC 2.2.1.2). In addition, the GRE3 gene encoding aldose reductase was deleted to further minimise xylitol production. Surprisingly the resulting strain grew anaerobically on xylose in synthetic media with a mu(max) as high as 0.09 h(-1) without any non-defined mutagenesis or selection. During growth on xylose, xylulose formation was absent and xylitol production was negligible. The specific xylose consumption rate in anaerobic xylose cultures was 1.1 g xylose (g biomass)(-1) h(-1). Mixtures of glucose and xylose were sequentially but completely consumed by anaerobic batch cultures, with glucose as the preferred substrate. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/15691745/Metabolic_engineering_of_a_xylose_isomerase_expressing_Saccharomyces_cerevisiae_strain_for_rapid_anaerobic_xylose_fermentation_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1016/j.femsyr.2004.09.010 DB - PRIME DP - Unbound Medicine ER -