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Changes in the metabolome of Saccharomyces cerevisiae associated with evolution in aerobic glucose-limited chemostats.
FEMS Yeast Res. 2005 Feb; 5(4-5):419-30.FY

Abstract

The effect of culture age on intra- and extracellular metabolite levels as well as on in vitro determined specific activities of enzymes of central carbon metabolism was investigated during evolution for over 90 generations of Saccharomyces cerevisiae CEN.PK 113-7D in an aerobic glucose/ethanol-limited chemostat at a specific dilution rate of 0.052 h(-1). It was found that the fluxes of consumed (O2, glucose/ethanol) and secreted compounds (CO2) did not change significantly during the entire cultivation period. However, morphological changes were observed, leading to an increased cellular surface area. During 90 generations of chemostat growth not only the residual glucose concentration decreased, also the intracellular concentrations of trehalose, glycolytic intermediates, TCA cycle intermediates and amino acids were found to have decreased with a factor 5-10. The only exception was glyoxylate which showed a fivefold increase in concentration. In addition to this the specific activities of most glycolytic enzymes also decreased by a factor 5-10 during long-term cultivation. Exceptions to this were hexokinase, phosphofructokinase, pyruvate kinase and 6-phosphogluconate dehydrogenase of which the activities remained unchanged. Furthermore, the concentrations of the adenylate nucleotides as well as the energy charge of the cells did not change in a significant manner. Surprisingly, the specific activities of glucose-6-phosphate dehydrogenase (G6PDH), malate synthase (MS) and isocitrate lyase (ICL) increased significantly during 90 generations of chemostat cultivation. These changes seem to indicate a pattern where metabolic overcapacities (for reversible reactions) and storage pools (trehalose, high levels of amino acids and excess protein in enzymes) are lost during the evolution period. The driving force is proposed to be a growth advantage in the absence of these metabolic overcapacities.

Authors+Show Affiliations

Department of Biotechnology, Faculty of Applied Sciences, Technical University of Delft, 67 Julianalaan, 2628 BC Delft, The Netherlands. m.mashego@tnw.tudelft.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15691747

Citation

Mashego, Mlawule R., et al. "Changes in the Metabolome of Saccharomyces Cerevisiae Associated With Evolution in Aerobic Glucose-limited Chemostats." FEMS Yeast Research, vol. 5, no. 4-5, 2005, pp. 419-30.
Mashego MR, Jansen ML, Vinke JL, et al. Changes in the metabolome of Saccharomyces cerevisiae associated with evolution in aerobic glucose-limited chemostats. FEMS Yeast Res. 2005;5(4-5):419-30.
Mashego, M. R., Jansen, M. L., Vinke, J. L., van Gulik, W. M., & Heijnen, J. J. (2005). Changes in the metabolome of Saccharomyces cerevisiae associated with evolution in aerobic glucose-limited chemostats. FEMS Yeast Research, 5(4-5), 419-30.
Mashego MR, et al. Changes in the Metabolome of Saccharomyces Cerevisiae Associated With Evolution in Aerobic Glucose-limited Chemostats. FEMS Yeast Res. 2005;5(4-5):419-30. PubMed PMID: 15691747.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in the metabolome of Saccharomyces cerevisiae associated with evolution in aerobic glucose-limited chemostats. AU - Mashego,Mlawule R, AU - Jansen,Mickel L A, AU - Vinke,Jacobus L, AU - van Gulik,Walter M, AU - Heijnen,Joseph J, PY - 2004/08/18/received PY - 2004/11/10/revised PY - 2004/11/10/accepted PY - 2005/2/5/pubmed PY - 2005/5/11/medline PY - 2005/2/5/entrez SP - 419 EP - 30 JF - FEMS yeast research JO - FEMS Yeast Res VL - 5 IS - 4-5 N2 - The effect of culture age on intra- and extracellular metabolite levels as well as on in vitro determined specific activities of enzymes of central carbon metabolism was investigated during evolution for over 90 generations of Saccharomyces cerevisiae CEN.PK 113-7D in an aerobic glucose/ethanol-limited chemostat at a specific dilution rate of 0.052 h(-1). It was found that the fluxes of consumed (O2, glucose/ethanol) and secreted compounds (CO2) did not change significantly during the entire cultivation period. However, morphological changes were observed, leading to an increased cellular surface area. During 90 generations of chemostat growth not only the residual glucose concentration decreased, also the intracellular concentrations of trehalose, glycolytic intermediates, TCA cycle intermediates and amino acids were found to have decreased with a factor 5-10. The only exception was glyoxylate which showed a fivefold increase in concentration. In addition to this the specific activities of most glycolytic enzymes also decreased by a factor 5-10 during long-term cultivation. Exceptions to this were hexokinase, phosphofructokinase, pyruvate kinase and 6-phosphogluconate dehydrogenase of which the activities remained unchanged. Furthermore, the concentrations of the adenylate nucleotides as well as the energy charge of the cells did not change in a significant manner. Surprisingly, the specific activities of glucose-6-phosphate dehydrogenase (G6PDH), malate synthase (MS) and isocitrate lyase (ICL) increased significantly during 90 generations of chemostat cultivation. These changes seem to indicate a pattern where metabolic overcapacities (for reversible reactions) and storage pools (trehalose, high levels of amino acids and excess protein in enzymes) are lost during the evolution period. The driving force is proposed to be a growth advantage in the absence of these metabolic overcapacities. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/15691747/Changes_in_the_metabolome_of_Saccharomyces_cerevisiae_associated_with_evolution_in_aerobic_glucose_limited_chemostats_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1016/j.femsyr.2004.11.008 DB - PRIME DP - Unbound Medicine ER -