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A synthetic human proline-rich-polypeptide enhances hydroxyl radical generation and fails to protect dopaminergic neurons against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced toxicity in mice.
Neurosci Lett. 2005 Mar 03; 375(3):187-91.NL

Abstract

Some of the proline-rich-polypeptides (PRPs) are shown to afford protection against spinal cord transection or crush syndrome-induced neurodegeneration in the brain. In the present study a synthetic proline-rich-polypeptide of human hypothalamus origin (h-PRP) has been examined for its potency to protect against dopaminergic neuronal damage caused by the parkinsonian neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Effect of h-PRP on hydroxyl radical (*OH) generation in a Fenton-like reaction was monitored, employing a sensitive salicylate hydroxylation procedure. Balb/c mice treated twice with MPTP (30 mg/kg. i.p., twice, 16 h apart) or h-PRP (20 microg/animal, twice, 16 h apart) showed significant loss of striatal dopamine as assayed by HPLC with electrochemical detection. h-PRP pretreatment failed to attenuate MPTP-induced striatal dopamine depletion. A dose-dependent increase in the generation of *OH by h-PRP suggests its pro-oxidant action, and explains its failure to protect against MPTP-induced parkinsonism in mice.

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Authors+Show Affiliations

Knaryan VH
H. Buniatian Institute of Biochemistry, Department of Neurohormones Biochemistry, National Academy of Sciences of the Republic of Armenia, 5/1 Paruir Sevak str., 375014 Yerevan, Republic of Armenia.
Samantaray S
No affiliation info available
Galoyan AA
No affiliation info available
Mohanakumar KP
No affiliation info available

MeSH

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridineAnimalsChromatography, High Pressure LiquidDopamineDose-Response Relationship, DrugDrug Administration ScheduleDrug InteractionsElectrochemistryHumansHydroxyl RadicalMPTP PoisoningMiceMice, Inbred BALB CNeuronsPeptidesProlineProline-Rich Protein DomainsTime FactorsTreatment Failure

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15694258

Citation

Knaryan, Varduhi H., et al. "A Synthetic Human Proline-rich-polypeptide Enhances Hydroxyl Radical Generation and Fails to Protect Dopaminergic Neurons Against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Toxicity in Mice." Neuroscience Letters, vol. 375, no. 3, 2005, pp. 187-91.
Knaryan VH, Samantaray S, Galoyan AA, et al. A synthetic human proline-rich-polypeptide enhances hydroxyl radical generation and fails to protect dopaminergic neurons against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced toxicity in mice. Neurosci Lett. 2005;375(3):187-91.
Knaryan, V. H., Samantaray, S., Galoyan, A. A., & Mohanakumar, K. P. (2005). A synthetic human proline-rich-polypeptide enhances hydroxyl radical generation and fails to protect dopaminergic neurons against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced toxicity in mice. Neuroscience Letters, 375(3), 187-91.
Knaryan VH, et al. A Synthetic Human Proline-rich-polypeptide Enhances Hydroxyl Radical Generation and Fails to Protect Dopaminergic Neurons Against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Toxicity in Mice. Neurosci Lett. 2005 Mar 3;375(3):187-91. PubMed PMID: 15694258.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A synthetic human proline-rich-polypeptide enhances hydroxyl radical generation and fails to protect dopaminergic neurons against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced toxicity in mice. AU - Knaryan,Varduhi H, AU - Samantaray,Supriti, AU - Galoyan,Armen A, AU - Mohanakumar,Kochupurackal P, PY - 2004/08/05/received PY - 2004/11/03/revised PY - 2004/11/03/accepted PY - 2005/2/8/pubmed PY - 2005/5/17/medline PY - 2005/2/8/entrez SP - 187 EP - 91 JF - Neuroscience letters JO - Neurosci Lett VL - 375 IS - 3 N2 - Some of the proline-rich-polypeptides (PRPs) are shown to afford protection against spinal cord transection or crush syndrome-induced neurodegeneration in the brain. In the present study a synthetic proline-rich-polypeptide of human hypothalamus origin (h-PRP) has been examined for its potency to protect against dopaminergic neuronal damage caused by the parkinsonian neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Effect of h-PRP on hydroxyl radical (*OH) generation in a Fenton-like reaction was monitored, employing a sensitive salicylate hydroxylation procedure. Balb/c mice treated twice with MPTP (30 mg/kg. i.p., twice, 16 h apart) or h-PRP (20 microg/animal, twice, 16 h apart) showed significant loss of striatal dopamine as assayed by HPLC with electrochemical detection. h-PRP pretreatment failed to attenuate MPTP-induced striatal dopamine depletion. A dose-dependent increase in the generation of *OH by h-PRP suggests its pro-oxidant action, and explains its failure to protect against MPTP-induced parkinsonism in mice. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/15694258/A_synthetic_human_proline_rich_polypeptide_enhances_hydroxyl_radical_generation_and_fails_to_protect_dopaminergic_neurons_against_1_methyl_4_phenyl_1236_tetrahydropyridine_induced_toxicity_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(04)01406-5 DB - PRIME DP - Unbound Medicine ER -