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Metabolic profiling of tryptophan-overproducing rice calli that express a feedback-insensitive alpha subunit of anthranilate synthase.
Plant Cell Physiol. 2005 Mar; 46(3):514-21.PC

Abstract

The profile of aromatic metabolites in calli was compared between wild-type rice (Oryza sativa cv. Nipponbare) and tryptophan-overproducing transgenic rice lines that express a gene (OASA1D) for a feedback-insensitive alpha subunit of anthranilate synthase. Metabolic profiling by high-performance liquid chromatography coupled with photodiode array detection of ultraviolet absorbance revealed a total of 71 peaks in both wild-type and transgenic calli. Only a limited effect on the pattern of major aromatic compounds was observed in tryptophan-accumulating transgenic rice lines, with the exception of an approximately 80-fold increase in the amount of tryptophan. Expression of OASA1D induced relatively small changes in several minor metabolites. One of the minor metabolites whose abundance was increased by OASA1D expression was purified and identified as a previously unknown indole-alkaloid glucoside. The levels of free and conjugated forms of indole-3-acetic acid (IAA), a plant hormone derived from the tryptophan biosynthetic pathway, were determined separately by liquid chromatography and tandem mass spectrometry (LC-MS/MS). The amounts of both free IAA and its conjugates were increased in the transgenic calli, suggesting that the activity of anthranilate synthase or the concentration of tryptophan (or both) is an important determinant of IAA biosynthesis.

Authors+Show Affiliations

Division of Applied Life Sciences, Department of Agriculture, Kyoto University, Kyoto, 606-8502 Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15695448

Citation

Morino, Keiko, et al. "Metabolic Profiling of Tryptophan-overproducing Rice Calli That Express a Feedback-insensitive Alpha Subunit of Anthranilate Synthase." Plant & Cell Physiology, vol. 46, no. 3, 2005, pp. 514-21.
Morino K, Matsuda F, Miyazawa H, et al. Metabolic profiling of tryptophan-overproducing rice calli that express a feedback-insensitive alpha subunit of anthranilate synthase. Plant Cell Physiol. 2005;46(3):514-21.
Morino, K., Matsuda, F., Miyazawa, H., Sukegawa, A., Miyagawa, H., & Wakasa, K. (2005). Metabolic profiling of tryptophan-overproducing rice calli that express a feedback-insensitive alpha subunit of anthranilate synthase. Plant & Cell Physiology, 46(3), 514-21.
Morino K, et al. Metabolic Profiling of Tryptophan-overproducing Rice Calli That Express a Feedback-insensitive Alpha Subunit of Anthranilate Synthase. Plant Cell Physiol. 2005;46(3):514-21. PubMed PMID: 15695448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic profiling of tryptophan-overproducing rice calli that express a feedback-insensitive alpha subunit of anthranilate synthase. AU - Morino,Keiko, AU - Matsuda,Fumio, AU - Miyazawa,Haruna, AU - Sukegawa,Akiko, AU - Miyagawa,Hisashi, AU - Wakasa,Kyo, Y1 - 2005/02/02/ PY - 2005/2/8/pubmed PY - 2005/8/16/medline PY - 2005/2/8/entrez SP - 514 EP - 21 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 46 IS - 3 N2 - The profile of aromatic metabolites in calli was compared between wild-type rice (Oryza sativa cv. Nipponbare) and tryptophan-overproducing transgenic rice lines that express a gene (OASA1D) for a feedback-insensitive alpha subunit of anthranilate synthase. Metabolic profiling by high-performance liquid chromatography coupled with photodiode array detection of ultraviolet absorbance revealed a total of 71 peaks in both wild-type and transgenic calli. Only a limited effect on the pattern of major aromatic compounds was observed in tryptophan-accumulating transgenic rice lines, with the exception of an approximately 80-fold increase in the amount of tryptophan. Expression of OASA1D induced relatively small changes in several minor metabolites. One of the minor metabolites whose abundance was increased by OASA1D expression was purified and identified as a previously unknown indole-alkaloid glucoside. The levels of free and conjugated forms of indole-3-acetic acid (IAA), a plant hormone derived from the tryptophan biosynthetic pathway, were determined separately by liquid chromatography and tandem mass spectrometry (LC-MS/MS). The amounts of both free IAA and its conjugates were increased in the transgenic calli, suggesting that the activity of anthranilate synthase or the concentration of tryptophan (or both) is an important determinant of IAA biosynthesis. SN - 0032-0781 UR - https://www.unboundmedicine.com/medline/citation/15695448/Metabolic_profiling_of_tryptophan_overproducing_rice_calli_that_express_a_feedback_insensitive_alpha_subunit_of_anthranilate_synthase_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pci051 DB - PRIME DP - Unbound Medicine ER -