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6-Oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl)fluorescein as a new fluorescent labeling reagent for aliphatic amines in environmental and food samples using high-performance liquid chromatography.
J Chromatogr A. 2005 Jan 21; 1063(1-2):143-51.JC

Abstract

6-Oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl)fluorescein (SAMF), a new fluorescein-based amine-reactive fluorescent probe was well designed, synthesized and used as a pre-column derivatizing reagent for the determination of aliphatic amines in HPLC. It exhibited relatively pH-independent fluorescence (pH 4-9) and excellent photostability. The derivatization was performed at room temperature in 6min. On a C18 column, the derivatives of SAMF with eight aliphatic amines were baseline separated in 28 min with a mobile phase of methanol-water (57:43, v/v) containing 10 mmol l(-1) pH 5.0, H3Cit3-NaOH buffer. With fluorescent detection at lambda(ex)/lambda(em) = 484/516 nm, the detection limit could reach 2-320 fmol (signal-to-noise = 3), which was equivalent to or better than the detection limits obtained from other analytical methods of aliphatic amines. The proposed method has been applied to the determination of the aliphatic amines in environmental and food samples such as lake water, red wine, white wine, and cheese with satisfying recoveries varying from 95 to 106%.

Authors+Show Affiliations

Department of Chemistry, Wuhan University, Wuhan 430072, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15700466

Citation

Cao, Li-Wei, et al. "6-Oxy-(N-succinimidyl Acetate)-9-(2'-methoxycarbonyl)fluorescein as a New Fluorescent Labeling Reagent for Aliphatic Amines in Environmental and Food Samples Using High-performance Liquid Chromatography." Journal of Chromatography. A, vol. 1063, no. 1-2, 2005, pp. 143-51.
Cao LW, Wang H, Li JS, et al. 6-Oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl)fluorescein as a new fluorescent labeling reagent for aliphatic amines in environmental and food samples using high-performance liquid chromatography. J Chromatogr A. 2005;1063(1-2):143-51.
Cao, L. W., Wang, H., Li, J. S., & Zhang, H. S. (2005). 6-Oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl)fluorescein as a new fluorescent labeling reagent for aliphatic amines in environmental and food samples using high-performance liquid chromatography. Journal of Chromatography. A, 1063(1-2), 143-51.
Cao LW, et al. 6-Oxy-(N-succinimidyl Acetate)-9-(2'-methoxycarbonyl)fluorescein as a New Fluorescent Labeling Reagent for Aliphatic Amines in Environmental and Food Samples Using High-performance Liquid Chromatography. J Chromatogr A. 2005 Jan 21;1063(1-2):143-51. PubMed PMID: 15700466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 6-Oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl)fluorescein as a new fluorescent labeling reagent for aliphatic amines in environmental and food samples using high-performance liquid chromatography. AU - Cao,Li-Wei, AU - Wang,Hong, AU - Li,Jin-Shu, AU - Zhang,Hua-Shan, PY - 2005/2/11/pubmed PY - 2005/5/19/medline PY - 2005/2/11/entrez SP - 143 EP - 51 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1063 IS - 1-2 N2 - 6-Oxy-(N-succinimidyl acetate)-9-(2'-methoxycarbonyl)fluorescein (SAMF), a new fluorescein-based amine-reactive fluorescent probe was well designed, synthesized and used as a pre-column derivatizing reagent for the determination of aliphatic amines in HPLC. It exhibited relatively pH-independent fluorescence (pH 4-9) and excellent photostability. The derivatization was performed at room temperature in 6min. On a C18 column, the derivatives of SAMF with eight aliphatic amines were baseline separated in 28 min with a mobile phase of methanol-water (57:43, v/v) containing 10 mmol l(-1) pH 5.0, H3Cit3-NaOH buffer. With fluorescent detection at lambda(ex)/lambda(em) = 484/516 nm, the detection limit could reach 2-320 fmol (signal-to-noise = 3), which was equivalent to or better than the detection limits obtained from other analytical methods of aliphatic amines. The proposed method has been applied to the determination of the aliphatic amines in environmental and food samples such as lake water, red wine, white wine, and cheese with satisfying recoveries varying from 95 to 106%. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/15700466/6_Oxy__N_succinimidyl_acetate__9__2'_methoxycarbonyl_fluorescein_as_a_new_fluorescent_labeling_reagent_for_aliphatic_amines_in_environmental_and_food_samples_using_high_performance_liquid_chromatography_ DB - PRIME DP - Unbound Medicine ER -
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