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[Effects of montelukast on apoptosis and Fas mRNA expression of eosinophils in airway of asthmatic guinea pigs].
Yao Xue Xue Bao. 2004 Oct; 39(10):769-73.YX

Abstract

AIM

To study the effect of montelukast on apoptosis and Fas mRNA expression of eosinophils in airway of asthmatic guinea pigs.

METHODS

Experimental asthma model of guinea pigs was induced by ovalbumin. The eosinophils in broncho-alveolar lavage fluid (BALF) were separated by density gradient centrifugation. The apoptosis of eosinophils was labeled by TdT-mediated dUTP nick end labeling (TUNEL) technique. Fas mRNA expression of eosinophils was detected by reverse transcription-polymerase chain reaction (RT-PCR).

RESULTS

After treatment with montelukast, the number of eosinophils in BALF of asthmatic guinea pigs decreased significantly. The apoptosis index as well as Fas mRNA expression of eosinophils were elevated significantly. There were statistical differences between the therapeutic group and the model group.

CONCLUSION

Apoptosis of eosinophils is highly correlated with the increased expression of Fas mRNA. Enhancing expression of Fas mRNA and promoting apoptosis of eosinophils subsequentely may be an important mechanism for montelukast to antagonize airway inflammation of asthma.

Authors+Show Affiliations

Medical and Pharmacological Institute of Yangzhou University, Yangzhou 225001, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15700813

Citation

Wu, Yu-qing, et al. "[Effects of Montelukast On Apoptosis and Fas mRNA Expression of Eosinophils in Airway of Asthmatic Guinea Pigs]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 39, no. 10, 2004, pp. 769-73.
Wu YQ, Zhou CH, Zhang HQ. [Effects of montelukast on apoptosis and Fas mRNA expression of eosinophils in airway of asthmatic guinea pigs]. Yao Xue Xue Bao. 2004;39(10):769-73.
Wu, Y. Q., Zhou, C. H., & Zhang, H. Q. (2004). [Effects of montelukast on apoptosis and Fas mRNA expression of eosinophils in airway of asthmatic guinea pigs]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 39(10), 769-73.
Wu YQ, Zhou CH, Zhang HQ. [Effects of Montelukast On Apoptosis and Fas mRNA Expression of Eosinophils in Airway of Asthmatic Guinea Pigs]. Yao Xue Xue Bao. 2004;39(10):769-73. PubMed PMID: 15700813.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of montelukast on apoptosis and Fas mRNA expression of eosinophils in airway of asthmatic guinea pigs]. AU - Wu,Yu-qing, AU - Zhou,Cheng-hua, AU - Zhang,Hong-quan, PY - 2005/2/11/pubmed PY - 2005/6/14/medline PY - 2005/2/11/entrez SP - 769 EP - 73 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 39 IS - 10 N2 - AIM: To study the effect of montelukast on apoptosis and Fas mRNA expression of eosinophils in airway of asthmatic guinea pigs. METHODS: Experimental asthma model of guinea pigs was induced by ovalbumin. The eosinophils in broncho-alveolar lavage fluid (BALF) were separated by density gradient centrifugation. The apoptosis of eosinophils was labeled by TdT-mediated dUTP nick end labeling (TUNEL) technique. Fas mRNA expression of eosinophils was detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: After treatment with montelukast, the number of eosinophils in BALF of asthmatic guinea pigs decreased significantly. The apoptosis index as well as Fas mRNA expression of eosinophils were elevated significantly. There were statistical differences between the therapeutic group and the model group. CONCLUSION: Apoptosis of eosinophils is highly correlated with the increased expression of Fas mRNA. Enhancing expression of Fas mRNA and promoting apoptosis of eosinophils subsequentely may be an important mechanism for montelukast to antagonize airway inflammation of asthma. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/15700813/[Effects_of_montelukast_on_apoptosis_and_Fas_mRNA_expression_of_eosinophils_in_airway_of_asthmatic_guinea_pigs]_ L2 - https://medlineplus.gov/asthma.html DB - PRIME DP - Unbound Medicine ER -