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A model for fluid secretion in Rhodnius upper Malpighian tubules (UMT).
J Membr Biol. 2004 Nov; 202(2):105-14.JM

Abstract

We have measured fluid secretion rate in Rhodnius prolixus upper Malpighian tubules (UMT) stimulated to secrete with 5-OH-tryptamine. We used double perfusions in order to have access separately to the basolateral and to the apical cell membranes. Thirteen pharmacological agents were applied: ouabain, Bafilomycin A(1), furosemide, bumetanide, DIOA, Probenecid, SITS, acetazolamide, amiloride, DPC, BaCl(2), pCMBS and DTT. These agents are known to block different ion transport functions, namely ATPases, co- and/or counter-transporters and ion and water channels. The basic assumption is that water movement changes reflect changes in ion transport mechanisms, which we localize as follows: (i) At the basolateral cell membrane, fundamental are a Na(+)-K(+)-2Cl(-) cotransporter and a Cl(-)-HCO(3) (-) exchanger; of intermediate importance are the Na(+)-K(+)-ATPase, Cl(-) channels and Rp-MIP water channels; K(+) channels play a lesser role: (ii) At the apical cell membrane, most important are a K(+)-Cl(-) cotransport that is being located for the first time, a V-H(+)-ATPase; and a Na(+)-H(+) exchanger; a urate-anion exchanger and K(+) channels are less important, while Cl(-) channels are not important at all. A tentative model for the function of the UMT cell is presented.

Authors+Show Affiliations

Instituto Venezolano de Investigaciones Científicas, IVIC, P. O. Box 21827, Caracas 1020-A, Venezuela. agutierr@ivic.veNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15702374

Citation

Gutiérrez, A M., et al. "A Model for Fluid Secretion in Rhodnius Upper Malpighian Tubules (UMT)." The Journal of Membrane Biology, vol. 202, no. 2, 2004, pp. 105-14.
Gutiérrez AM, Hernández CS, Whittembury G. A model for fluid secretion in Rhodnius upper Malpighian tubules (UMT). J Membr Biol. 2004;202(2):105-14.
Gutiérrez, A. M., Hernández, C. S., & Whittembury, G. (2004). A model for fluid secretion in Rhodnius upper Malpighian tubules (UMT). The Journal of Membrane Biology, 202(2), 105-14.
Gutiérrez AM, Hernández CS, Whittembury G. A Model for Fluid Secretion in Rhodnius Upper Malpighian Tubules (UMT). J Membr Biol. 2004;202(2):105-14. PubMed PMID: 15702374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A model for fluid secretion in Rhodnius upper Malpighian tubules (UMT). AU - Gutiérrez,A M, AU - Hernández,C S, AU - Whittembury,G, PY - 2004/05/03/received PY - 2004/09/30/revised PY - 2005/2/11/pubmed PY - 2005/5/13/medline PY - 2005/2/11/entrez SP - 105 EP - 14 JF - The Journal of membrane biology JO - J Membr Biol VL - 202 IS - 2 N2 - We have measured fluid secretion rate in Rhodnius prolixus upper Malpighian tubules (UMT) stimulated to secrete with 5-OH-tryptamine. We used double perfusions in order to have access separately to the basolateral and to the apical cell membranes. Thirteen pharmacological agents were applied: ouabain, Bafilomycin A(1), furosemide, bumetanide, DIOA, Probenecid, SITS, acetazolamide, amiloride, DPC, BaCl(2), pCMBS and DTT. These agents are known to block different ion transport functions, namely ATPases, co- and/or counter-transporters and ion and water channels. The basic assumption is that water movement changes reflect changes in ion transport mechanisms, which we localize as follows: (i) At the basolateral cell membrane, fundamental are a Na(+)-K(+)-2Cl(-) cotransporter and a Cl(-)-HCO(3) (-) exchanger; of intermediate importance are the Na(+)-K(+)-ATPase, Cl(-) channels and Rp-MIP water channels; K(+) channels play a lesser role: (ii) At the apical cell membrane, most important are a K(+)-Cl(-) cotransport that is being located for the first time, a V-H(+)-ATPase; and a Na(+)-H(+) exchanger; a urate-anion exchanger and K(+) channels are less important, while Cl(-) channels are not important at all. A tentative model for the function of the UMT cell is presented. SN - 0022-2631 UR - https://www.unboundmedicine.com/medline/citation/15702374/A_model_for_fluid_secretion_in_Rhodnius_upper_Malpighian_tubules__UMT__ L2 - https://dx.doi.org/10.1007/s00232-004-0723-6 DB - PRIME DP - Unbound Medicine ER -