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CpG island methylator phenotype is a strong determinant of poor prognosis in neuroblastomas.
Cancer Res. 2005 Feb 01; 65(3):828-34.CR

Abstract

Neuroblastoma, one of the most common pediatric solid tumors, is characterized by two extreme disease courses, spontaneous regression and life-threatening progression. Here, we conducted a genome-wide search for differences in DNA methylation that distinguish between neuroblastomas of the two types. Three CpG islands (CGI) and two groups of CGIs were found to be methylated specifically in neuroblastomas with a poor prognosis. By quantitative analysis of 140 independent cases, methylation of all the five CGI (groups) was shown to be closely associated with each other, conforming to the CpG island methylator phenotype (CIMP) concept. The presence of CIMP was sensitively detected by methylation of the PCDHB CGIs and associated with significantly poor survival (hazard ratio, 22.1; 95% confidence interval, 5.3-93.4; P < 0.0001). Almost all cases with N-myc amplification (37 of 38 cases) exhibited CIMP. Even in 102 cases without N-myc amplification, the presence of CIMP (30 cases) strongly predicted poor survival (hazard ratio, 12.4; 95% confidence interval, 2.6-58.9; P = 0.002). Methylation of PCDHB CGIs, located in their gene bodies, did not suppress gene expression or induce histone modifications. However, CIMP was significantly associated with methylation of promoter CGIs of the RASSF1A and BLU tumor suppressor genes. The results showed that neuroblastomas with CIMP have a poor prognosis and suggested induction of silencing of important genes as an underlying mechanism.

Authors+Show Affiliations

Carcinogenesis Division, National Cancer Center Research Institute, University of Tokyo Graduate School of Medicine, 5-1-1 Tsikiji, Chuo-ku, Tokyo 104-0045, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15705880

Citation

Abe, Masanobu, et al. "CpG Island Methylator Phenotype Is a Strong Determinant of Poor Prognosis in Neuroblastomas." Cancer Research, vol. 65, no. 3, 2005, pp. 828-34.
Abe M, Ohira M, Kaneda A, et al. CpG island methylator phenotype is a strong determinant of poor prognosis in neuroblastomas. Cancer Res. 2005;65(3):828-34.
Abe, M., Ohira, M., Kaneda, A., Yagi, Y., Yamamoto, S., Kitano, Y., Takato, T., Nakagawara, A., & Ushijima, T. (2005). CpG island methylator phenotype is a strong determinant of poor prognosis in neuroblastomas. Cancer Research, 65(3), 828-34.
Abe M, et al. CpG Island Methylator Phenotype Is a Strong Determinant of Poor Prognosis in Neuroblastomas. Cancer Res. 2005 Feb 1;65(3):828-34. PubMed PMID: 15705880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CpG island methylator phenotype is a strong determinant of poor prognosis in neuroblastomas. AU - Abe,Masanobu, AU - Ohira,Miki, AU - Kaneda,Atsushi, AU - Yagi,Yukiko, AU - Yamamoto,Seiichiro, AU - Kitano,Yoshihiro, AU - Takato,Tsuyoshi, AU - Nakagawara,Akira, AU - Ushijima,Toshikazu, PY - 2005/2/12/pubmed PY - 2005/3/15/medline PY - 2005/2/12/entrez SP - 828 EP - 34 JF - Cancer research JO - Cancer Res VL - 65 IS - 3 N2 - Neuroblastoma, one of the most common pediatric solid tumors, is characterized by two extreme disease courses, spontaneous regression and life-threatening progression. Here, we conducted a genome-wide search for differences in DNA methylation that distinguish between neuroblastomas of the two types. Three CpG islands (CGI) and two groups of CGIs were found to be methylated specifically in neuroblastomas with a poor prognosis. By quantitative analysis of 140 independent cases, methylation of all the five CGI (groups) was shown to be closely associated with each other, conforming to the CpG island methylator phenotype (CIMP) concept. The presence of CIMP was sensitively detected by methylation of the PCDHB CGIs and associated with significantly poor survival (hazard ratio, 22.1; 95% confidence interval, 5.3-93.4; P < 0.0001). Almost all cases with N-myc amplification (37 of 38 cases) exhibited CIMP. Even in 102 cases without N-myc amplification, the presence of CIMP (30 cases) strongly predicted poor survival (hazard ratio, 12.4; 95% confidence interval, 2.6-58.9; P = 0.002). Methylation of PCDHB CGIs, located in their gene bodies, did not suppress gene expression or induce histone modifications. However, CIMP was significantly associated with methylation of promoter CGIs of the RASSF1A and BLU tumor suppressor genes. The results showed that neuroblastomas with CIMP have a poor prognosis and suggested induction of silencing of important genes as an underlying mechanism. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/15705880/CpG_island_methylator_phenotype_is_a_strong_determinant_of_poor_prognosis_in_neuroblastomas_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&amp;pmid=15705880 DB - PRIME DP - Unbound Medicine ER -