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Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection.
Electrophoresis. 2005 Mar; 26(6):1071-9.E

Abstract

Serotonin or 5-hydroxytryptamine (5-HT) is a major neurotransmitter in the central nervous system. In this work, a method for analyzing 5-HT in brain microdialysis samples using a commercially available capillary electrophoresis (CE) system has been developed. A pH-mediated in-capillary preconcentration of samples was performed, and after separation by capillary zone electrophoresis, native fluorescence of 5-HT was detected by a 266 nm solid-state laser. The separation conditions for the analysis of 5-HT in standard solutions and microdialysates have been optimized, and this method has been validated on both pharmacological and analytical bases. Separation of 5-HT was performed using a 80 mmol/L citrate buffer, pH 2.5, containing 20 mmol/L hydroxypropyl-beta-cyclodextrin (HP-beta-CD) and +30 kV voltage. The detection limit was 2.5 x 10(-10) mol/L. This method allows the in vivo brain monitoring of 5-HT using a simple, accurate CE measurement in underivatized microdialysis samples.

Authors+Show Affiliations

Laboratoire de Neuropharmacologie et Neurochimie, INSERM U512, Institut Fédératif des Neurosciences de Lyon (IFR 19), Université Claude Bernard, F-69373 Lyon Cedex 08, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

15706576

Citation

Benturquia, Nadia, et al. "Analysis of Serotonin in Brain Microdialysates Using Capillary Electrophoresis and Native Laser-induced Fluorescence Detection." Electrophoresis, vol. 26, no. 6, 2005, pp. 1071-9.
Benturquia N, Couderc F, Sauvinet V, et al. Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection. Electrophoresis. 2005;26(6):1071-9.
Benturquia, N., Couderc, F., Sauvinet, V., Orset, C., Parrot, S., Bayle, C., Renaud, B., & Denoroy, L. (2005). Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection. Electrophoresis, 26(6), 1071-9.
Benturquia N, et al. Analysis of Serotonin in Brain Microdialysates Using Capillary Electrophoresis and Native Laser-induced Fluorescence Detection. Electrophoresis. 2005;26(6):1071-9. PubMed PMID: 15706576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection. AU - Benturquia,Nadia, AU - Couderc,François, AU - Sauvinet,Valérie, AU - Orset,Cyrille, AU - Parrot,Sandrine, AU - Bayle,Christophe, AU - Renaud,Bernard, AU - Denoroy,Luc, PY - 2005/2/12/pubmed PY - 2005/8/6/medline PY - 2005/2/12/entrez SP - 1071 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 26 IS - 6 N2 - Serotonin or 5-hydroxytryptamine (5-HT) is a major neurotransmitter in the central nervous system. In this work, a method for analyzing 5-HT in brain microdialysis samples using a commercially available capillary electrophoresis (CE) system has been developed. A pH-mediated in-capillary preconcentration of samples was performed, and after separation by capillary zone electrophoresis, native fluorescence of 5-HT was detected by a 266 nm solid-state laser. The separation conditions for the analysis of 5-HT in standard solutions and microdialysates have been optimized, and this method has been validated on both pharmacological and analytical bases. Separation of 5-HT was performed using a 80 mmol/L citrate buffer, pH 2.5, containing 20 mmol/L hydroxypropyl-beta-cyclodextrin (HP-beta-CD) and +30 kV voltage. The detection limit was 2.5 x 10(-10) mol/L. This method allows the in vivo brain monitoring of 5-HT using a simple, accurate CE measurement in underivatized microdialysis samples. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15706576/Analysis_of_serotonin_in_brain_microdialysates_using_capillary_electrophoresis_and_native_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/elps.200410150 DB - PRIME DP - Unbound Medicine ER -