[Determination of flavoniod-glycosides in Yixintong pills by HPLC].Zhongguo Zhong Yao Za Zhi. 2004 Jun; 29(6):525-7.ZZ
To develop an HPLC method for determining flavoniod-glycosides in Yixintong pills.
Agilent ZORBAX Extend-C18 (4.6 mm x 250 mm, 5 microm) was used with tetrahydrofuran-methanol-acetonitrile-water-acetic acid (30:3:4:120:3) as mobile phase. Detection wavelength was 330 nm.
Good linearities of rhamnosylvitexin and vetexin-glucoside were obtained within the range of 0.044-1.78 microg (r = 0.9999) and 0.042-0.85 microg (r = 0.9999); the average recoveries were 100.0% and 99.2%; RSD were 0.92% and 1.8%, respectively.
This method can be used for quality control of Yixintong pills.