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Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species.
Life Sci. 2005 Mar 11; 76(17):1953-64.LS

Abstract

The antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of seven Hedyotisspecies were investigated. The antioxidant activity was evaluated by the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods while the radical scavenging activity was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The anti-inflammatory activity related to NO inhibition of the plant extracts was measured by the Griess assay while cytotoxicity were measured by the MTT assay against CEM-SS cell line. The antibacterial bioassay (against 4 bacteria, i.e. Bacillus subtilis B28 (mutant), Bacillus subtilis B29 (wild-type), Pseudomonas aeruginosa UI 60690 and methicillin resistant Staphylococcus aureus, (MRSA) was also carried out using the disc-diffusion method. All tested extracts exhibited very strong antioxidant properties when compared to Vitamin E (alpha-tocopherol) with percent inhibition of 89-98% in the FTC and 60-95% in the TBA assays. In the DPPH method, H. herbacea exhibited the strongest radical scavenging activity with an IC50 value of 32 microg/ml. The results from the Griess assay showed that the tested extracts are weak inhibitors of NO synthase. However, all tested extracts exhibited moderate cytotoxic properties against CEM-SS cell line giving CD50 values in the range of 21-41 microg/ml. In the antibacterial bioassay, the stems and the roots of H. capitellata showed moderate activity against the 4 tested bacteria while the leaves showed moderate activity towards B. subtilis B28, MRSA and P. aeruginosa only. The roots of H. dichotoma showed strong antibacterial activity against all 4 bacteria. All other extracts did not exhibit any antibacterial activity.

Authors+Show Affiliations

Faculty of Applied Science, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15707878

Citation

Ahmad, Rohaya, et al. "Antioxidant, Radical-scavenging, Anti-inflammatory, Cytotoxic and Antibacterial Activities of Methanolic Extracts of some Hedyotis Species." Life Sciences, vol. 76, no. 17, 2005, pp. 1953-64.
Ahmad R, Ali AM, Israf DA, et al. Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species. Life Sci. 2005;76(17):1953-64.
Ahmad, R., Ali, A. M., Israf, D. A., Ismail, N. H., Shaari, K., & Lajis, N. H. (2005). Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species. Life Sciences, 76(17), 1953-64.
Ahmad R, et al. Antioxidant, Radical-scavenging, Anti-inflammatory, Cytotoxic and Antibacterial Activities of Methanolic Extracts of some Hedyotis Species. Life Sci. 2005 Mar 11;76(17):1953-64. PubMed PMID: 15707878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species. AU - Ahmad,Rohaya, AU - Ali,Abdul Manaf, AU - Israf,Daud A, AU - Ismail,Nor Hadiani, AU - Shaari,Khozirah, AU - Lajis,Nordin Hj, Y1 - 2005/01/27/ PY - 2004/04/19/received PY - 2004/08/10/accepted PY - 2005/2/15/pubmed PY - 2005/3/16/medline PY - 2005/2/15/entrez SP - 1953 EP - 64 JF - Life sciences JO - Life Sci VL - 76 IS - 17 N2 - The antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of seven Hedyotisspecies were investigated. The antioxidant activity was evaluated by the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods while the radical scavenging activity was measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The anti-inflammatory activity related to NO inhibition of the plant extracts was measured by the Griess assay while cytotoxicity were measured by the MTT assay against CEM-SS cell line. The antibacterial bioassay (against 4 bacteria, i.e. Bacillus subtilis B28 (mutant), Bacillus subtilis B29 (wild-type), Pseudomonas aeruginosa UI 60690 and methicillin resistant Staphylococcus aureus, (MRSA) was also carried out using the disc-diffusion method. All tested extracts exhibited very strong antioxidant properties when compared to Vitamin E (alpha-tocopherol) with percent inhibition of 89-98% in the FTC and 60-95% in the TBA assays. In the DPPH method, H. herbacea exhibited the strongest radical scavenging activity with an IC50 value of 32 microg/ml. The results from the Griess assay showed that the tested extracts are weak inhibitors of NO synthase. However, all tested extracts exhibited moderate cytotoxic properties against CEM-SS cell line giving CD50 values in the range of 21-41 microg/ml. In the antibacterial bioassay, the stems and the roots of H. capitellata showed moderate activity against the 4 tested bacteria while the leaves showed moderate activity towards B. subtilis B28, MRSA and P. aeruginosa only. The roots of H. dichotoma showed strong antibacterial activity against all 4 bacteria. All other extracts did not exhibit any antibacterial activity. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/15707878/Antioxidant_radical_scavenging_anti_inflammatory_cytotoxic_and_antibacterial_activities_of_methanolic_extracts_of_some_Hedyotis_species_ DB - PRIME DP - Unbound Medicine ER -