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Detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei and Burkholderia thailandensis by multiplex PCR.
FEMS Immunol Med Microbiol. 2005 Mar 01; 43(3):413-7.FI

Abstract

Burkholderia pseudomallei, a Gram-negative bacterium that causes melioidosis may be differentiated from closely related species of Burkholderia mallei that causes glanders and non-pathogenic species of Burkholderia thailandensis by multiplex PCR. The multiplex PCR consists of primers that flank a 10-bp repetitive element in B. pseudomallei and B. mallei amplifying PCR fragment of varying sizes between 400-700 bp, a unique sequence in B. thailandensis amplifying a PCR fragment of 308 bp and the metalloprotease gene amplifying a PCR fragment of 245 bp in B. pseudomallei and B. thailandensis. The multiplex PCR not only can differentiate the three Burkholderia species but can also be used for epidemiological typing of B. pseudomallei and B. mallei strains.

Authors+Show Affiliations

Centre for BioMedical Sciences, Defence Medical and Environment Research Institute, DSO National Laboratories 27 Medical Drive, Singapore 117510, Singapore. lmayann@dso.org.sgNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15708316

Citation

Lee, May-Ann, et al. "Detection and Differentiation of Burkholderia Pseudomallei, Burkholderia Mallei and Burkholderia Thailandensis By Multiplex PCR." FEMS Immunology and Medical Microbiology, vol. 43, no. 3, 2005, pp. 413-7.
Lee MA, Wang D, Yap EH. Detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei and Burkholderia thailandensis by multiplex PCR. FEMS Immunol Med Microbiol. 2005;43(3):413-7.
Lee, M. A., Wang, D., & Yap, E. H. (2005). Detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei and Burkholderia thailandensis by multiplex PCR. FEMS Immunology and Medical Microbiology, 43(3), 413-7.
Lee MA, Wang D, Yap EH. Detection and Differentiation of Burkholderia Pseudomallei, Burkholderia Mallei and Burkholderia Thailandensis By Multiplex PCR. FEMS Immunol Med Microbiol. 2005 Mar 1;43(3):413-7. PubMed PMID: 15708316.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei and Burkholderia thailandensis by multiplex PCR. AU - Lee,May-Ann, AU - Wang,Dongling, AU - Yap,Eu Hian, PY - 2004/05/31/received PY - 2004/08/13/revised PY - 2004/10/22/accepted PY - 2005/2/15/pubmed PY - 2005/4/19/medline PY - 2005/2/15/entrez SP - 413 EP - 7 JF - FEMS immunology and medical microbiology JO - FEMS Immunol Med Microbiol VL - 43 IS - 3 N2 - Burkholderia pseudomallei, a Gram-negative bacterium that causes melioidosis may be differentiated from closely related species of Burkholderia mallei that causes glanders and non-pathogenic species of Burkholderia thailandensis by multiplex PCR. The multiplex PCR consists of primers that flank a 10-bp repetitive element in B. pseudomallei and B. mallei amplifying PCR fragment of varying sizes between 400-700 bp, a unique sequence in B. thailandensis amplifying a PCR fragment of 308 bp and the metalloprotease gene amplifying a PCR fragment of 245 bp in B. pseudomallei and B. thailandensis. The multiplex PCR not only can differentiate the three Burkholderia species but can also be used for epidemiological typing of B. pseudomallei and B. mallei strains. SN - 0928-8244 UR - https://www.unboundmedicine.com/medline/citation/15708316/Detection_and_differentiation_of_Burkholderia_pseudomallei_Burkholderia_mallei_and_Burkholderia_thailandensis_by_multiplex_PCR_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0928-8244&date=2005&volume=43&issue=3&spage=413 DB - PRIME DP - Unbound Medicine ER -