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Fabrication of DNA-protein conjugate layer on gold-substrate and its application to immunosensor.
Colloids Surf B Biointerfaces. 2005 Feb 25; 40(3-4):173-7.CS

Abstract

The fabrication of antibody thin film using both protein G and oligonucleotide was carried out by self-assembly (SA) technique for immunosensor. A mixture of 11-mercaptoundecanoic acid (MUA) and oligonucleotide with thiol (SH) end group was self-assembled of gold (Au) surface for two-dimensional (2D) configuration. Protein G was chemically adsorbed on the 11-MUA surface, and then the antibody was immobilized on the protein G region. On the immobilized single-stranded DNA, the complementary DNA-antibody conjugate was hybridized for the oriented immobilization of antibody. The formation of self-assembled 11-MUA/oligonucleotide layer, protein G immobilization, antibody layer, and antigen binding was investigated using surface plasmon resonance (SPR). The topographies of the fabricated surfaces were observed by atomic force microscopy (AFM). When compared with the amount of antigen binding on the antibody thin film fabricated by protein G only, the proposed biosurface fabricated with both protein G and oligonucleotide showed better binding capacity, which implicates the improvement of the detection limit.

Authors+Show Affiliations

Choi JW
Department of Chemical and Biomolecular Engineering, Sogang University, Mapo-Gu, Seoul 121 742, Republic of Korea. jwchoi@ccs.sogang.ac.kr
Chun BS
No affiliation info available
Oh BK
No affiliation info available
Lee W
No affiliation info available
Lee WH
No affiliation info available

MeSH

AntibodiesDNAGoldInsulinMicroscopy, Atomic ForceNerve Tissue ProteinsSurface Plasmon Resonance

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15708509

Citation

Choi, Jeong-Woo, et al. "Fabrication of DNA-protein Conjugate Layer On Gold-substrate and Its Application to Immunosensor." Colloids and Surfaces. B, Biointerfaces, vol. 40, no. 3-4, 2005, pp. 173-7.
Choi JW, Chun BS, Oh BK, et al. Fabrication of DNA-protein conjugate layer on gold-substrate and its application to immunosensor. Colloids Surf B Biointerfaces. 2005;40(3-4):173-7.
Choi, J. W., Chun, B. S., Oh, B. K., Lee, W., & Lee, W. H. (2005). Fabrication of DNA-protein conjugate layer on gold-substrate and its application to immunosensor. Colloids and Surfaces. B, Biointerfaces, 40(3-4), 173-7.
Choi JW, et al. Fabrication of DNA-protein Conjugate Layer On Gold-substrate and Its Application to Immunosensor. Colloids Surf B Biointerfaces. 2005 Feb 25;40(3-4):173-7. PubMed PMID: 15708509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fabrication of DNA-protein conjugate layer on gold-substrate and its application to immunosensor. AU - Choi,Jeong-Woo, AU - Chun,Bum Suk, AU - Oh,Byung-Keun, AU - Lee,Woochang, AU - Lee,Won Hong, PY - 2005/2/15/pubmed PY - 2005/6/10/medline PY - 2005/2/15/entrez SP - 173 EP - 7 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 40 IS - 3-4 N2 - The fabrication of antibody thin film using both protein G and oligonucleotide was carried out by self-assembly (SA) technique for immunosensor. A mixture of 11-mercaptoundecanoic acid (MUA) and oligonucleotide with thiol (SH) end group was self-assembled of gold (Au) surface for two-dimensional (2D) configuration. Protein G was chemically adsorbed on the 11-MUA surface, and then the antibody was immobilized on the protein G region. On the immobilized single-stranded DNA, the complementary DNA-antibody conjugate was hybridized for the oriented immobilization of antibody. The formation of self-assembled 11-MUA/oligonucleotide layer, protein G immobilization, antibody layer, and antigen binding was investigated using surface plasmon resonance (SPR). The topographies of the fabricated surfaces were observed by atomic force microscopy (AFM). When compared with the amount of antigen binding on the antibody thin film fabricated by protein G only, the proposed biosurface fabricated with both protein G and oligonucleotide showed better binding capacity, which implicates the improvement of the detection limit. SN - 0927-7765 UR - https://www.unboundmedicine.com/medline/citation/15708509/Fabrication_of_DNA_protein_conjugate_layer_on_gold_substrate_and_its_application_to_immunosensor_ DB - PRIME DP - Unbound Medicine ER -