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Characterization of microglia induced from mouse embryonic stem cells and their migration into the brain parenchyma.
J Neuroimmunol 2005; 160(1-2):210-8JN

Abstract

We derived microglia from mouse embryonic stem cells (ES cells) at very high density. Using the markers Mac1(+)/CD45(low) and Mac1(+)/CD45(high) to define microglia and macrophages, respectively, we show that Mac1(+) cells are induced by GM-CSF stimulation following neuronal differentiation of mouse ES cells using a five-step method. CD45(low) expression was high and CD45(high) expression was low on induced cells. We used a density gradient method to obtain a large amount of microglia-like cells, approximately 90% of Mac1(+) cells. Microglia-like cells expressed MHC class I, class II, CD40, CD80, CD86, and IFN-gammaR. The expression level of these molecules on microglia-like cells was barely enhanced by IFN-gamma. Intravenously transferred GFP(+) microglia derived from GFP(+) ES cells selectively accumulated in brain but not in peripheral tissues such as spleen and lymph node. GFP(+) cells were detected mainly in corpus callosum and hippocampus but were rarely seen in cerebral cortex, where Iba1, another marker of microglia, is primarily expressed. Furthermore, both GFP(+) and Iba1(+) cells exhibited a ramified morphology characteristic of mature microglia. These studies suggest that ES cell-derived microglia-like cells obtained using our protocol are functional and migrate selectively into the brain but not into peripheral tissues after intravenous transplantation.

Authors+Show Affiliations

Department of Neurosurgery, Kochi Medical School, Kochi University, Kohasu, Okoh-cho, Nankoku, Kochi 783-8505, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15710475

Citation

Tsuchiya, Takahiro, et al. "Characterization of Microglia Induced From Mouse Embryonic Stem Cells and Their Migration Into the Brain Parenchyma." Journal of Neuroimmunology, vol. 160, no. 1-2, 2005, pp. 210-8.
Tsuchiya T, Park KC, Toyonaga S, et al. Characterization of microglia induced from mouse embryonic stem cells and their migration into the brain parenchyma. J Neuroimmunol. 2005;160(1-2):210-8.
Tsuchiya, T., Park, K. C., Toyonaga, S., Yamada, S. M., Nakabayashi, H., Nakai, E., ... Shimizu, K. (2005). Characterization of microglia induced from mouse embryonic stem cells and their migration into the brain parenchyma. Journal of Neuroimmunology, 160(1-2), pp. 210-8.
Tsuchiya T, et al. Characterization of Microglia Induced From Mouse Embryonic Stem Cells and Their Migration Into the Brain Parenchyma. J Neuroimmunol. 2005;160(1-2):210-8. PubMed PMID: 15710475.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of microglia induced from mouse embryonic stem cells and their migration into the brain parenchyma. AU - Tsuchiya,Takahiro, AU - Park,Kae Chang, AU - Toyonaga,Shinichi, AU - Yamada,Shoko M, AU - Nakabayashi,Hiromichi, AU - Nakai,Eiichi, AU - Ikawa,Naoki, AU - Furuya,Masato, AU - Tominaga,Akira, AU - Shimizu,Keiji, Y1 - 2004/12/24/ PY - 2004/09/13/received PY - 2004/10/25/revised PY - 2004/10/25/accepted PY - 2005/2/16/pubmed PY - 2005/4/29/medline PY - 2005/2/16/entrez SP - 210 EP - 8 JF - Journal of neuroimmunology JO - J. Neuroimmunol. VL - 160 IS - 1-2 N2 - We derived microglia from mouse embryonic stem cells (ES cells) at very high density. Using the markers Mac1(+)/CD45(low) and Mac1(+)/CD45(high) to define microglia and macrophages, respectively, we show that Mac1(+) cells are induced by GM-CSF stimulation following neuronal differentiation of mouse ES cells using a five-step method. CD45(low) expression was high and CD45(high) expression was low on induced cells. We used a density gradient method to obtain a large amount of microglia-like cells, approximately 90% of Mac1(+) cells. Microglia-like cells expressed MHC class I, class II, CD40, CD80, CD86, and IFN-gammaR. The expression level of these molecules on microglia-like cells was barely enhanced by IFN-gamma. Intravenously transferred GFP(+) microglia derived from GFP(+) ES cells selectively accumulated in brain but not in peripheral tissues such as spleen and lymph node. GFP(+) cells were detected mainly in corpus callosum and hippocampus but were rarely seen in cerebral cortex, where Iba1, another marker of microglia, is primarily expressed. Furthermore, both GFP(+) and Iba1(+) cells exhibited a ramified morphology characteristic of mature microglia. These studies suggest that ES cell-derived microglia-like cells obtained using our protocol are functional and migrate selectively into the brain but not into peripheral tissues after intravenous transplantation. SN - 0165-5728 UR - https://www.unboundmedicine.com/medline/citation/15710475/Characterization_of_microglia_induced_from_mouse_embryonic_stem_cells_and_their_migration_into_the_brain_parenchyma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-5728(04)00404-7 DB - PRIME DP - Unbound Medicine ER -