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Determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxymethamphetamine in urine by online solid-phase extraction and ion-pairing liquid chromatography with detection by electrospray tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2005; 19(6):775-80.RC

Abstract

A method using an online solid-phase extraction (SPE) and ion-pairing liquid chromatography with electrospray tandem mass spectrometry (LC/ES-MS/MS) was developed for determination of amphetamine (Amp), methamphetamine (mAmp), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxyethylamphetamine (MDEA), and 3,4-methylenedioxymethamphetamine (MDMA) in urine samples. A SPE cartridge column with both hydrophilic and lipophilic functions was utilized for online extraction. A reversed-phase C18 LC column was employed for LC separation and MS/MS was used for detection. Trifluoroacetic acid was added to the mobile phase as an ion-pairing reagent. This method was fully automated and the extraction and analysis procedures were controlled by a six-port switch valve. Recoveries ranging from 85-101% were measured. Good linear ranges (10-500 ng/mL) for Amp and mAmp were determined. For MDA, MDMA and MDEA, dual linear ranges were obtained from 5-100 and 100-500 ng/mL, respectively. The detection limit of each analytical compound, based on a signal-to-noise ratio of 3, ranged from 1-3 ng/mL. The applicability of this newly developed method was examined by analyzing several urine samples from drug users. Good agreement was obtained between the results from this method and a literature GC/MS method.

Authors+Show Affiliations

P.O. Box 86-72, Department of Chemistry, Soochow University, Taipei, Taiwan.No affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15712289

Citation

Wu, Ti-Yu, and Ming-Ren Fuh. "Determination of Amphetamine, Methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxymethamphetamine in Urine By Online Solid-phase Extraction and Ion-pairing Liquid Chromatography With Detection By Electrospray Tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 19, no. 6, 2005, pp. 775-80.
Wu TY, Fuh MR. Determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxymethamphetamine in urine by online solid-phase extraction and ion-pairing liquid chromatography with detection by electrospray tandem mass spectrometry. Rapid Commun Mass Spectrom. 2005;19(6):775-80.
Wu, T. Y., & Fuh, M. R. (2005). Determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxymethamphetamine in urine by online solid-phase extraction and ion-pairing liquid chromatography with detection by electrospray tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 19(6), 775-80.
Wu TY, Fuh MR. Determination of Amphetamine, Methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxymethamphetamine in Urine By Online Solid-phase Extraction and Ion-pairing Liquid Chromatography With Detection By Electrospray Tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2005;19(6):775-80. PubMed PMID: 15712289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxymethamphetamine in urine by online solid-phase extraction and ion-pairing liquid chromatography with detection by electrospray tandem mass spectrometry. AU - Wu,Ti-Yu, AU - Fuh,Ming-Ren, PY - 2005/2/16/pubmed PY - 2005/5/13/medline PY - 2005/2/16/entrez SP - 775 EP - 80 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 19 IS - 6 N2 - A method using an online solid-phase extraction (SPE) and ion-pairing liquid chromatography with electrospray tandem mass spectrometry (LC/ES-MS/MS) was developed for determination of amphetamine (Amp), methamphetamine (mAmp), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxyethylamphetamine (MDEA), and 3,4-methylenedioxymethamphetamine (MDMA) in urine samples. A SPE cartridge column with both hydrophilic and lipophilic functions was utilized for online extraction. A reversed-phase C18 LC column was employed for LC separation and MS/MS was used for detection. Trifluoroacetic acid was added to the mobile phase as an ion-pairing reagent. This method was fully automated and the extraction and analysis procedures were controlled by a six-port switch valve. Recoveries ranging from 85-101% were measured. Good linear ranges (10-500 ng/mL) for Amp and mAmp were determined. For MDA, MDMA and MDEA, dual linear ranges were obtained from 5-100 and 100-500 ng/mL, respectively. The detection limit of each analytical compound, based on a signal-to-noise ratio of 3, ranged from 1-3 ng/mL. The applicability of this newly developed method was examined by analyzing several urine samples from drug users. Good agreement was obtained between the results from this method and a literature GC/MS method. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/15712289/Determination_of_amphetamine_methamphetamine_34_methylenedioxyamphetamine_34_methylenedioxyethylamphetamine_and_34_methylenedioxymethamphetamine_in_urine_by_online_solid_phase_extraction_and_ion_pairing_liquid_chromatography_with_detection_by_electrospray_tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.1856 DB - PRIME DP - Unbound Medicine ER -