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Comparative study of the comet assay and the micronucleus test in amphibian larvae (Xenopus laevis) using benzo(a)pyrene, ethyl methanesulfonate, and methyl methanesulfonate: establishment of a positive control in the amphibian comet assay.
Environ Toxicol. 2005 Feb; 20(1):74-84.ET

Abstract

The present investigation explored the potential use of the comet assay (CA) as a genotoxicity test in the amphibian Xenopus laevis and compared it with the French standard micronucleus test (MNT). Benzo[a]pyrene (B[a]P), methyl methanesulfonate (MMS), and ethyl methanesulfonate (EMS) were used as model compounds for assessing DNA damage. Damage levels were measured as DNA strand breaks after alkaline electrophoresis of nuclei isolated from larval amphibian erythrocytes using the CA in order to establish a positive control for further ecotoxicological investigations. The results led to the selection of MMS as a positive control on the basis of the higher sensitivity of Xenopus laevis to this compound. The CA and MNT were compared for their ability to detect DNA damage with the doses of chemical agents and exposure times applied. EMS and MMS were shown to increase micronucleus and DNA strand break formation in larval erythrocytes concurrently. However, B[a]P increased micronucleus formation but not that of DNA strand breaks. Time-dose experiments over 12 days of exposure suggest that the CA provides an earlier significant response to genotoxicants than does the MNT. In Xenopus the CA appears to be a sensitive and suitable method for detecting genotoxicity like that caused by EMS and MMS. It can be considered a genotoxicity-screening tool. The results for B[a]P show that both tests should be used in a complementary manner on Xenopus.

Authors+Show Affiliations

Laboratoire d'Ecologie des Hydrosystèmes-LEH, FRE CNRS UPS 2630, Centre Universitaire de Formation et de Recherche Jean-François Champollion, Campus d'Albi, place de Verdun, 81012 Albi cedex 9, France. florence.mouchet@wanadoo.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15712291

Citation

Mouchet, F, et al. "Comparative Study of the Comet Assay and the Micronucleus Test in Amphibian Larvae (Xenopus Laevis) Using Benzo(a)pyrene, Ethyl Methanesulfonate, and Methyl Methanesulfonate: Establishment of a Positive Control in the Amphibian Comet Assay." Environmental Toxicology, vol. 20, no. 1, 2005, pp. 74-84.
Mouchet F, Gauthier L, Mailhes C, et al. Comparative study of the comet assay and the micronucleus test in amphibian larvae (Xenopus laevis) using benzo(a)pyrene, ethyl methanesulfonate, and methyl methanesulfonate: establishment of a positive control in the amphibian comet assay. Environ Toxicol. 2005;20(1):74-84.
Mouchet, F., Gauthier, L., Mailhes, C., Ferrier, V., & Devaux, A. (2005). Comparative study of the comet assay and the micronucleus test in amphibian larvae (Xenopus laevis) using benzo(a)pyrene, ethyl methanesulfonate, and methyl methanesulfonate: establishment of a positive control in the amphibian comet assay. Environmental Toxicology, 20(1), 74-84.
Mouchet F, et al. Comparative Study of the Comet Assay and the Micronucleus Test in Amphibian Larvae (Xenopus Laevis) Using Benzo(a)pyrene, Ethyl Methanesulfonate, and Methyl Methanesulfonate: Establishment of a Positive Control in the Amphibian Comet Assay. Environ Toxicol. 2005;20(1):74-84. PubMed PMID: 15712291.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative study of the comet assay and the micronucleus test in amphibian larvae (Xenopus laevis) using benzo(a)pyrene, ethyl methanesulfonate, and methyl methanesulfonate: establishment of a positive control in the amphibian comet assay. AU - Mouchet,F, AU - Gauthier,L, AU - Mailhes,C, AU - Ferrier,V, AU - Devaux,A, PY - 2005/2/16/pubmed PY - 2005/6/4/medline PY - 2005/2/16/entrez SP - 74 EP - 84 JF - Environmental toxicology JO - Environ Toxicol VL - 20 IS - 1 N2 - The present investigation explored the potential use of the comet assay (CA) as a genotoxicity test in the amphibian Xenopus laevis and compared it with the French standard micronucleus test (MNT). Benzo[a]pyrene (B[a]P), methyl methanesulfonate (MMS), and ethyl methanesulfonate (EMS) were used as model compounds for assessing DNA damage. Damage levels were measured as DNA strand breaks after alkaline electrophoresis of nuclei isolated from larval amphibian erythrocytes using the CA in order to establish a positive control for further ecotoxicological investigations. The results led to the selection of MMS as a positive control on the basis of the higher sensitivity of Xenopus laevis to this compound. The CA and MNT were compared for their ability to detect DNA damage with the doses of chemical agents and exposure times applied. EMS and MMS were shown to increase micronucleus and DNA strand break formation in larval erythrocytes concurrently. However, B[a]P increased micronucleus formation but not that of DNA strand breaks. Time-dose experiments over 12 days of exposure suggest that the CA provides an earlier significant response to genotoxicants than does the MNT. In Xenopus the CA appears to be a sensitive and suitable method for detecting genotoxicity like that caused by EMS and MMS. It can be considered a genotoxicity-screening tool. The results for B[a]P show that both tests should be used in a complementary manner on Xenopus. SN - 1520-4081 UR - https://www.unboundmedicine.com/medline/citation/15712291/Comparative_study_of_the_comet_assay_and_the_micronucleus_test_in_amphibian_larvae__Xenopus_laevis__using_benzo_a_pyrene_ethyl_methanesulfonate_and_methyl_methanesulfonate:_establishment_of_a_positive_control_in_the_amphibian_comet_assay_ L2 - https://doi.org/10.1002/tox.20080 DB - PRIME DP - Unbound Medicine ER -