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RP-HPLC analysis of the phenolic compounds of plant extracts. investigation of their antioxidant capacity and antimicrobial activity.
J Agric Food Chem. 2005 Feb 23; 53(4):1190-5.JA

Abstract

Extracts of aromatic plants of Greek origin were examined as potential sources of phenolic compounds. RP-HPLC with UV detection was employed for the identification and quantification of the phenolic antioxidants, present in methanolic extracts. The most abundant phenolic acids were ferulic acid (1.1-280 mg/100 g of dry sample) and caffeic acid (1.2-60 mg/100 g of dry sample). (+)-Catechin and quercetin were the most abundant flavonoids. Apigenin and luteolin were detected in high amounts in Menta pulegium and Thymus vulgaris, respectively. The antioxidant capacity was determined, in dried ground plants and in their methanol extracts, with the Rancimat test using sunflower oil as substrate. Both pulverized plants and extracts showed antioxidant capacity. Total phenolic content in the extracts was determined spectrometrically according to the Folin-Ciocalteu assay and ranged from 1 to 21 mg of gallic acid/100 g of dry sample. Antimicrobial activity of the extracts against selected microbes was also conducted in this study.

Authors+Show Affiliations

Laboratory of Food Chemistry, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15713039

Citation

Proestos, C, et al. "RP-HPLC Analysis of the Phenolic Compounds of Plant Extracts. Investigation of Their Antioxidant Capacity and Antimicrobial Activity." Journal of Agricultural and Food Chemistry, vol. 53, no. 4, 2005, pp. 1190-5.
Proestos C, Chorianopoulos N, Nychas GJ, et al. RP-HPLC analysis of the phenolic compounds of plant extracts. investigation of their antioxidant capacity and antimicrobial activity. J Agric Food Chem. 2005;53(4):1190-5.
Proestos, C., Chorianopoulos, N., Nychas, G. J., & Komaitis, M. (2005). RP-HPLC analysis of the phenolic compounds of plant extracts. investigation of their antioxidant capacity and antimicrobial activity. Journal of Agricultural and Food Chemistry, 53(4), 1190-5.
Proestos C, et al. RP-HPLC Analysis of the Phenolic Compounds of Plant Extracts. Investigation of Their Antioxidant Capacity and Antimicrobial Activity. J Agric Food Chem. 2005 Feb 23;53(4):1190-5. PubMed PMID: 15713039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RP-HPLC analysis of the phenolic compounds of plant extracts. investigation of their antioxidant capacity and antimicrobial activity. AU - Proestos,C, AU - Chorianopoulos,N, AU - Nychas,G-J E, AU - Komaitis,M, PY - 2005/2/17/pubmed PY - 2005/3/31/medline PY - 2005/2/17/entrez SP - 1190 EP - 5 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 53 IS - 4 N2 - Extracts of aromatic plants of Greek origin were examined as potential sources of phenolic compounds. RP-HPLC with UV detection was employed for the identification and quantification of the phenolic antioxidants, present in methanolic extracts. The most abundant phenolic acids were ferulic acid (1.1-280 mg/100 g of dry sample) and caffeic acid (1.2-60 mg/100 g of dry sample). (+)-Catechin and quercetin were the most abundant flavonoids. Apigenin and luteolin were detected in high amounts in Menta pulegium and Thymus vulgaris, respectively. The antioxidant capacity was determined, in dried ground plants and in their methanol extracts, with the Rancimat test using sunflower oil as substrate. Both pulverized plants and extracts showed antioxidant capacity. Total phenolic content in the extracts was determined spectrometrically according to the Folin-Ciocalteu assay and ranged from 1 to 21 mg of gallic acid/100 g of dry sample. Antimicrobial activity of the extracts against selected microbes was also conducted in this study. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15713039/RP_HPLC_analysis_of_the_phenolic_compounds_of_plant_extracts__investigation_of_their_antioxidant_capacity_and_antimicrobial_activity_ L2 - https://doi.org/10.1021/jf040083t DB - PRIME DP - Unbound Medicine ER -