Tags

Type your tag names separated by a space and hit enter

The nuclear exosome contributes to autogenous control of NAB2 mRNA levels.
Mol Cell Biol. 2005 Mar; 25(5):1577-85.MC

Abstract

The RNA-processing exosome is a complex of riboexonucleases required for 3'-end formation of some noncoding RNAs and for the degradation of mRNAs in eukaryotes. The nuclear form of the exosome functions in an mRNA surveillance pathway that retains and degrades improperly processed precursor mRNAs within the nucleus. We report here that the nuclear exosome controls the level of NAB2 mRNA, encoding the nuclear poly(A)+-RNA-binding protein Nab2p. Mutations affecting the activity of the nuclear, but not the cytoplasmic, exosome cause an increase in the amount of NAB2 mRNA. Cis- and trans-acting mutations that inhibit degradation by the nuclear-exosome subunit Rrp6p result in elevated levels of NAB2 mRNA. Control of NAB2 mRNA levels occurs posttranscriptionally and requires a sequence of 26 consecutive adenosines (A26) in the NAB2 3' untranslated region, which represses NAB2 3'-end formation and sensitizes the transcript to degradation by Rrp6p. Analysis of NAB2 mRNA levels in a nab2-1 mutant and in the presence of excess Nab2p indicates that Nab2p activity negatively controls NAB2 mRNA levels in an A26- and Rrp6p-dependent manner. These findings suggest a novel regulatory circuit in which the nuclear exosome controls the level of NAB2 mRNA in response to changes in the activity of Nab2 protein.

Authors+Show Affiliations

Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Box 672, 601 Elmwood Ave., Rochester, NY 14642, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15713618

Citation

Roth, Kelly M., et al. "The Nuclear Exosome Contributes to Autogenous Control of NAB2 mRNA Levels." Molecular and Cellular Biology, vol. 25, no. 5, 2005, pp. 1577-85.
Roth KM, Wolf MK, Rossi M, et al. The nuclear exosome contributes to autogenous control of NAB2 mRNA levels. Mol Cell Biol. 2005;25(5):1577-85.
Roth, K. M., Wolf, M. K., Rossi, M., & Butler, J. S. (2005). The nuclear exosome contributes to autogenous control of NAB2 mRNA levels. Molecular and Cellular Biology, 25(5), 1577-85.
Roth KM, et al. The Nuclear Exosome Contributes to Autogenous Control of NAB2 mRNA Levels. Mol Cell Biol. 2005;25(5):1577-85. PubMed PMID: 15713618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The nuclear exosome contributes to autogenous control of NAB2 mRNA levels. AU - Roth,Kelly M, AU - Wolf,Maria K, AU - Rossi,Marie, AU - Butler,J Scott, PY - 2005/2/17/pubmed PY - 2005/3/29/medline PY - 2005/2/17/entrez SP - 1577 EP - 85 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 25 IS - 5 N2 - The RNA-processing exosome is a complex of riboexonucleases required for 3'-end formation of some noncoding RNAs and for the degradation of mRNAs in eukaryotes. The nuclear form of the exosome functions in an mRNA surveillance pathway that retains and degrades improperly processed precursor mRNAs within the nucleus. We report here that the nuclear exosome controls the level of NAB2 mRNA, encoding the nuclear poly(A)+-RNA-binding protein Nab2p. Mutations affecting the activity of the nuclear, but not the cytoplasmic, exosome cause an increase in the amount of NAB2 mRNA. Cis- and trans-acting mutations that inhibit degradation by the nuclear-exosome subunit Rrp6p result in elevated levels of NAB2 mRNA. Control of NAB2 mRNA levels occurs posttranscriptionally and requires a sequence of 26 consecutive adenosines (A26) in the NAB2 3' untranslated region, which represses NAB2 3'-end formation and sensitizes the transcript to degradation by Rrp6p. Analysis of NAB2 mRNA levels in a nab2-1 mutant and in the presence of excess Nab2p indicates that Nab2p activity negatively controls NAB2 mRNA levels in an A26- and Rrp6p-dependent manner. These findings suggest a novel regulatory circuit in which the nuclear exosome controls the level of NAB2 mRNA in response to changes in the activity of Nab2 protein. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/15713618/The_nuclear_exosome_contributes_to_autogenous_control_of_NAB2_mRNA_levels_ DB - PRIME DP - Unbound Medicine ER -