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Fate of Escherichia coli O157:H7 in irrigation water on soils and plants as validated by culture method and real-time PCR.
Can J Microbiol. 2004 Dec; 50(12):1007-14.CJ

Abstract

One of the most common vehicles by which Escherichia coli O157:H7 may be introduced into crops is contaminated irrigation water. Water contamination is becoming more common in rural areas of the United States as a result of large animal operations, and up to 40% of tested drinking-water wells are contaminated with E. coli. In this study, 2 contrasting soil samples were inoculated with E. coli O157:H7 expressing green fluorescent protein through irrigation water. Real-time PCR and culture methods were used to quantify the fate of this pathogen in phyllosphere (leaf surface), rhizosphere (volume of soil tightly held by plant roots), and non-rhizosphere soils. A real-time PCR assay was designed with the eae gene of E. coli O157:H7. The probe was incorporated into real-time PCR containing DNA extracted from the phyllosphere, rhizosphere, and non-rhizosphere soils. The detection limit for E. coli O157:H7 quantification by real-time PCR was 1.2 x 10(3) in the rhizosphere, phyllosphere, and non-rhizosphere samples. E. coli O157:H7 concentrations were higher in the rhizosphere than in the non-rhizosphere soils and leaf surfaces, and persisted longer in clay soil. The persistence of E. coli O157:H7 in phyllosphere, rhizosphere, and non-rhizosphere soils over 45 days may play a significant part in the recontamination cycle of produce in the environment. Therefore, the rapidity of the real-time PCR assay may be a useful tool for quantification and monitoring of E. coli O157:H7 in irrigation water and on contaminated fresh produce.

Authors+Show Affiliations

Ibekwe AM
USDA-ARS, George E. Brown Jr. Salinity Lab, Riverside, CA 92507, USA. aibekwe@ussl.ars.usda.gov
Watt PM
No affiliation info available
Shouse PJ
No affiliation info available
Grieve CM
No affiliation info available

MeSH

Adhesins, BacterialColony Count, MicrobialDNA, BacterialEscherichia coli O157Escherichia coli ProteinsFood MicrobiologyPlant LeavesPlant RootsPolymerase Chain ReactionSoil MicrobiologyWater Microbiology

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15714231

Citation

Ibekwe, A Mark, et al. "Fate of Escherichia Coli O157:H7 in Irrigation Water On Soils and Plants as Validated By Culture Method and Real-time PCR." Canadian Journal of Microbiology, vol. 50, no. 12, 2004, pp. 1007-14.
Ibekwe AM, Watt PM, Shouse PJ, et al. Fate of Escherichia coli O157:H7 in irrigation water on soils and plants as validated by culture method and real-time PCR. Can J Microbiol. 2004;50(12):1007-14.
Ibekwe, A. M., Watt, P. M., Shouse, P. J., & Grieve, C. M. (2004). Fate of Escherichia coli O157:H7 in irrigation water on soils and plants as validated by culture method and real-time PCR. Canadian Journal of Microbiology, 50(12), 1007-14.
Ibekwe AM, et al. Fate of Escherichia Coli O157:H7 in Irrigation Water On Soils and Plants as Validated By Culture Method and Real-time PCR. Can J Microbiol. 2004;50(12):1007-14. PubMed PMID: 15714231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fate of Escherichia coli O157:H7 in irrigation water on soils and plants as validated by culture method and real-time PCR. AU - Ibekwe,A Mark, AU - Watt,Pamela M, AU - Shouse,Peter J, AU - Grieve,Catherine M, PY - 2005/2/17/pubmed PY - 2005/6/10/medline PY - 2005/2/17/entrez SP - 1007 EP - 14 JF - Canadian journal of microbiology JO - Can J Microbiol VL - 50 IS - 12 N2 - One of the most common vehicles by which Escherichia coli O157:H7 may be introduced into crops is contaminated irrigation water. Water contamination is becoming more common in rural areas of the United States as a result of large animal operations, and up to 40% of tested drinking-water wells are contaminated with E. coli. In this study, 2 contrasting soil samples were inoculated with E. coli O157:H7 expressing green fluorescent protein through irrigation water. Real-time PCR and culture methods were used to quantify the fate of this pathogen in phyllosphere (leaf surface), rhizosphere (volume of soil tightly held by plant roots), and non-rhizosphere soils. A real-time PCR assay was designed with the eae gene of E. coli O157:H7. The probe was incorporated into real-time PCR containing DNA extracted from the phyllosphere, rhizosphere, and non-rhizosphere soils. The detection limit for E. coli O157:H7 quantification by real-time PCR was 1.2 x 10(3) in the rhizosphere, phyllosphere, and non-rhizosphere samples. E. coli O157:H7 concentrations were higher in the rhizosphere than in the non-rhizosphere soils and leaf surfaces, and persisted longer in clay soil. The persistence of E. coli O157:H7 in phyllosphere, rhizosphere, and non-rhizosphere soils over 45 days may play a significant part in the recontamination cycle of produce in the environment. Therefore, the rapidity of the real-time PCR assay may be a useful tool for quantification and monitoring of E. coli O157:H7 in irrigation water and on contaminated fresh produce. SN - 0008-4166 UR - https://www.unboundmedicine.com/medline/citation/15714231/Fate_of_Escherichia_coli_O157:H7_in_irrigation_water_on_soils_and_plants_as_validated_by_culture_method_and_real_time_PCR_ DB - PRIME DP - Unbound Medicine ER -