Tags

Type your tag names separated by a space and hit enter

Changes in retinal neuronal populations in the DBA/2J mouse.
Cell Tissue Res 2005; 320(1):51-9CT

Abstract

DBA/2J (D2) mice develop a form of progressive pigmentary glaucoma with increasing age. We have compared retinal cell populations of D2 mice with those in control C57BL/6J mice to provide information on retinal histopathology in the D2 mouse. The D2 mouse retina is characterized by a reduction in retinal thickness caused mainly by a thinning of the inner retinal layers. Immunocytochemical staining for specific inner retinal neuronal markers, viz., calbindin for horizontal cells; protein kinase C (PKC) and recoverin for bipolar cells, glycine, gamma-aminobutyric acid (GABA), choline acetyltransferase (ChAT), and nitric oxide synthase (NOS) for amacrine cells, and osteopontin (OPN) for ganglion cells, was performed to detect preferentially affected neurons in the D2 mouse retina. Calbindin, PKC, and recoverin immunoreactivities were not significantly altered. Amacrine cells immunoreactive for GABA, ChAT, and OPN were markedly decreased in number, whereas NOS-immunoreactive amacrine cells increased in number. However, no changes were observed in the population of glycine-immunoreactive amacrine cells. These findings indicate a significant loss of retinal ganglion and some amacrine cells, whereas glycinergic amacrine cells, horizontal, and bipolar cells are almost unaffected in the D2 mouse. The reduction in amacrine cells appears to be attributable to a loss of GABAergic and particularly cholinergic amacrine cells. The increase in nitrergic neurons with the consequent increase in NOS and NO may be important in the changes in the retinal organization that lead to glaucomain D2 mice. Thus, the D2 mouse retina represents a useful model for studying the pathogenesis of glaucoma and mechanisms of retinal neuronal death and for evaluating neuroprotection strategies.

Authors+Show Affiliations

Department of Ophthalmology, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15714280

Citation

Moon, Jung-Il, et al. "Changes in Retinal Neuronal Populations in the DBA/2J Mouse." Cell and Tissue Research, vol. 320, no. 1, 2005, pp. 51-9.
Moon JI, Kim IB, Gwon JS, et al. Changes in retinal neuronal populations in the DBA/2J mouse. Cell Tissue Res. 2005;320(1):51-9.
Moon, J. I., Kim, I. B., Gwon, J. S., Park, M. H., Kang, T. H., Lim, E. J., ... Chun, M. H. (2005). Changes in retinal neuronal populations in the DBA/2J mouse. Cell and Tissue Research, 320(1), pp. 51-9.
Moon JI, et al. Changes in Retinal Neuronal Populations in the DBA/2J Mouse. Cell Tissue Res. 2005;320(1):51-9. PubMed PMID: 15714280.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in retinal neuronal populations in the DBA/2J mouse. AU - Moon,Jung-Il, AU - Kim,In-Beom, AU - Gwon,Jae-Sung, AU - Park,Myoung-Hee, AU - Kang,Tae-Hoon, AU - Lim,Eun-Jin, AU - Choi,Kyu-Ryong, AU - Chun,Myung-Hoon, Y1 - 2005/02/16/ PY - 2004/10/06/received PY - 2004/11/26/accepted PY - 2005/2/17/pubmed PY - 2005/7/22/medline PY - 2005/2/17/entrez SP - 51 EP - 9 JF - Cell and tissue research JO - Cell Tissue Res. VL - 320 IS - 1 N2 - DBA/2J (D2) mice develop a form of progressive pigmentary glaucoma with increasing age. We have compared retinal cell populations of D2 mice with those in control C57BL/6J mice to provide information on retinal histopathology in the D2 mouse. The D2 mouse retina is characterized by a reduction in retinal thickness caused mainly by a thinning of the inner retinal layers. Immunocytochemical staining for specific inner retinal neuronal markers, viz., calbindin for horizontal cells; protein kinase C (PKC) and recoverin for bipolar cells, glycine, gamma-aminobutyric acid (GABA), choline acetyltransferase (ChAT), and nitric oxide synthase (NOS) for amacrine cells, and osteopontin (OPN) for ganglion cells, was performed to detect preferentially affected neurons in the D2 mouse retina. Calbindin, PKC, and recoverin immunoreactivities were not significantly altered. Amacrine cells immunoreactive for GABA, ChAT, and OPN were markedly decreased in number, whereas NOS-immunoreactive amacrine cells increased in number. However, no changes were observed in the population of glycine-immunoreactive amacrine cells. These findings indicate a significant loss of retinal ganglion and some amacrine cells, whereas glycinergic amacrine cells, horizontal, and bipolar cells are almost unaffected in the D2 mouse. The reduction in amacrine cells appears to be attributable to a loss of GABAergic and particularly cholinergic amacrine cells. The increase in nitrergic neurons with the consequent increase in NOS and NO may be important in the changes in the retinal organization that lead to glaucomain D2 mice. Thus, the D2 mouse retina represents a useful model for studying the pathogenesis of glaucoma and mechanisms of retinal neuronal death and for evaluating neuroprotection strategies. SN - 0302-766X UR - https://www.unboundmedicine.com/medline/citation/15714280/Changes_in_retinal_neuronal_populations_in_the_DBA/2J_mouse_ L2 - https://dx.doi.org/10.1007/s00441-004-1062-8 DB - PRIME DP - Unbound Medicine ER -