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Connections between integrins and Rac GTPase pathways control gonad formation and function in C. elegans.
Biochim Biophys Acta. 2005 May 25; 1723(1-3):248-55.BB

Abstract

The integrins are a family of alphabeta heterodimeric transmembrane receptors that link extracellular matrix (ECM) proteins to the cytoskeleton and orchestrate cell behaviors. It's been suggested that integrins interact with Rho family small GTPases, such as Rho and Rac. We took advantage of a C. elegans nematode line expressing HA-betatail, a beta integrin transgene inhibiting the functions of endogenous integrins, to determine the combined effects of reducing PAT-3 beta integrin and Rac pathway activities. Double mutants of HA-betatail and unc-73, a guanine nucleotide exchange factor GEF for MIG-2/Rac, had body wall and vulval muscle abnormalities. On the other hand, HA-betatail combined with mutant CED-5, another Rac interacting protein, showed ovulation defects and sterility. RNA-mediated interference (RNAi) of pat-3 on Rac mutant backgrounds also affected gonad structure and function. These results show a functional link between integrins and Rac signaling in muscles and gonads. Furthermore, data showing distinct phenotypes of HA-betatail with unc-73 versus ced-5 suggest some tissue-specificity in the usage of Rac signaling pathways.

Authors+Show Affiliations

Department of Biology, Baylor University, One Bear Place 97388, Waco, TX 76798, United States. Myeongwoo_Lee@baylor.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15716039

Citation

Lee, Myeongwoo, et al. "Connections Between Integrins and Rac GTPase Pathways Control Gonad Formation and Function in C. Elegans." Biochimica Et Biophysica Acta, vol. 1723, no. 1-3, 2005, pp. 248-55.
Lee M, Shen B, Schwarzbauer JE, et al. Connections between integrins and Rac GTPase pathways control gonad formation and function in C. elegans. Biochim Biophys Acta. 2005;1723(1-3):248-55.
Lee, M., Shen, B., Schwarzbauer, J. E., Ahn, J., & Kwon, J. (2005). Connections between integrins and Rac GTPase pathways control gonad formation and function in C. elegans. Biochimica Et Biophysica Acta, 1723(1-3), 248-55.
Lee M, et al. Connections Between Integrins and Rac GTPase Pathways Control Gonad Formation and Function in C. Elegans. Biochim Biophys Acta. 2005 May 25;1723(1-3):248-55. PubMed PMID: 15716039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Connections between integrins and Rac GTPase pathways control gonad formation and function in C. elegans. AU - Lee,Myeongwoo, AU - Shen,Bing, AU - Schwarzbauer,Jean E, AU - Ahn,Jeong, AU - Kwon,John, Y1 - 2005/01/22/ PY - 2004/11/18/received PY - 2005/01/07/revised PY - 2005/01/07/accepted PY - 2005/2/18/pubmed PY - 2005/7/13/medline PY - 2005/2/18/entrez SP - 248 EP - 55 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1723 IS - 1-3 N2 - The integrins are a family of alphabeta heterodimeric transmembrane receptors that link extracellular matrix (ECM) proteins to the cytoskeleton and orchestrate cell behaviors. It's been suggested that integrins interact with Rho family small GTPases, such as Rho and Rac. We took advantage of a C. elegans nematode line expressing HA-betatail, a beta integrin transgene inhibiting the functions of endogenous integrins, to determine the combined effects of reducing PAT-3 beta integrin and Rac pathway activities. Double mutants of HA-betatail and unc-73, a guanine nucleotide exchange factor GEF for MIG-2/Rac, had body wall and vulval muscle abnormalities. On the other hand, HA-betatail combined with mutant CED-5, another Rac interacting protein, showed ovulation defects and sterility. RNA-mediated interference (RNAi) of pat-3 on Rac mutant backgrounds also affected gonad structure and function. These results show a functional link between integrins and Rac signaling in muscles and gonads. Furthermore, data showing distinct phenotypes of HA-betatail with unc-73 versus ced-5 suggest some tissue-specificity in the usage of Rac signaling pathways. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/15716039/Connections_between_integrins_and_Rac_GTPase_pathways_control_gonad_formation_and_function_in_C__elegans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4165(05)00009-7 DB - PRIME DP - Unbound Medicine ER -