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Isolation and characterization of novel xrs2 mutations in Saccharomyces cerevisiae.
Genetics. 2005 May; 170(1):71-85.G

Abstract

The Mre11/Rad50/Xrs2 (MRX) complex is involved in DNA damage repair, DNA damage response, telomere control, and meiotic recombination. Here, we constructed and characterized novel mutant alleles of XRS2. The alleles with mutations in the C-terminal conserved domain of Xrs2 were grouped into the same class. Mutant Xrs2 in this class lacked Mre11 interaction ability. The second class, lacking a C-terminal end, showed defects only in telomere control. A previous study showed that this C-terminal end contains a Tel1-association domain. These results indicate that Xrs2 contains two functional domains, Mre11- and Tel1-binding domains. While the Mre11-binding domain is essential for Xrs2 function, the Tel1-binding domain may be essential only for Tel1 function in telomere maintenance. The third class, despite containing a large deletion in the N-terminal region, showed no defects in DNA damage repair. However, some mutants, which showed a reduced level of Xrs2 protein, were partially defective in formation of meiotic DSBs and telomere maintenance. These defects were suppressed by overexpression of the mutant Xrs2 protein. This result suggests that the total amount of Xrs2 protein is a critical determinant for the function of the MRX complex especially with regard to telomere maintenance and meiotic DSB formation.

Authors+Show Affiliations

Department of Radiation Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15716496

Citation

Shima, Hiroki, et al. "Isolation and Characterization of Novel Xrs2 Mutations in Saccharomyces Cerevisiae." Genetics, vol. 170, no. 1, 2005, pp. 71-85.
Shima H, Suzuki M, Shinohara M. Isolation and characterization of novel xrs2 mutations in Saccharomyces cerevisiae. Genetics. 2005;170(1):71-85.
Shima, H., Suzuki, M., & Shinohara, M. (2005). Isolation and characterization of novel xrs2 mutations in Saccharomyces cerevisiae. Genetics, 170(1), 71-85.
Shima H, Suzuki M, Shinohara M. Isolation and Characterization of Novel Xrs2 Mutations in Saccharomyces Cerevisiae. Genetics. 2005;170(1):71-85. PubMed PMID: 15716496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of novel xrs2 mutations in Saccharomyces cerevisiae. AU - Shima,Hiroki, AU - Suzuki,Masakatu, AU - Shinohara,Miki, Y1 - 2005/02/16/ PY - 2005/2/18/pubmed PY - 2006/6/7/medline PY - 2005/2/18/entrez SP - 71 EP - 85 JF - Genetics JO - Genetics VL - 170 IS - 1 N2 - The Mre11/Rad50/Xrs2 (MRX) complex is involved in DNA damage repair, DNA damage response, telomere control, and meiotic recombination. Here, we constructed and characterized novel mutant alleles of XRS2. The alleles with mutations in the C-terminal conserved domain of Xrs2 were grouped into the same class. Mutant Xrs2 in this class lacked Mre11 interaction ability. The second class, lacking a C-terminal end, showed defects only in telomere control. A previous study showed that this C-terminal end contains a Tel1-association domain. These results indicate that Xrs2 contains two functional domains, Mre11- and Tel1-binding domains. While the Mre11-binding domain is essential for Xrs2 function, the Tel1-binding domain may be essential only for Tel1 function in telomere maintenance. The third class, despite containing a large deletion in the N-terminal region, showed no defects in DNA damage repair. However, some mutants, which showed a reduced level of Xrs2 protein, were partially defective in formation of meiotic DSBs and telomere maintenance. These defects were suppressed by overexpression of the mutant Xrs2 protein. This result suggests that the total amount of Xrs2 protein is a critical determinant for the function of the MRX complex especially with regard to telomere maintenance and meiotic DSB formation. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/15716496/Isolation_and_characterization_of_novel_xrs2_mutations_in_Saccharomyces_cerevisiae_ L2 - https://academic.oup.com/genetics/article-lookup/doi/10.1534/genetics.104.037580 DB - PRIME DP - Unbound Medicine ER -