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Dynamics of cytomegalovirus (CMV)-specific T cells in HIV-1-infected individuals progressing to AIDS with CMV end-organ disease.
J Infect Dis. 2005 Mar 15; 191(6):873-80.JI

Abstract

BACKGROUND

Since cytomegalovirus (CMV) infection can cause serious clinical complications in immunocompromised individuals, we assessed cellular immune requirements for protection against CMV end-organ disease (CMV-EOD) in human immunodeficiency virus type 1 (HIV-1) infection.

METHODS

Longitudinal samples from HIV-1-infected patients in the Amsterdam cohort were analyzed. Dynamics of CMV-specific CD8(+) and CD4(+) T cell responses were analyzed by 4-color fluorescence analysis using major histocompatibility class I CMV peptide-tetramers and by intracellular staining for perforin, granzyme B, and interferon (IFN)- gamma after stimulation with CMV-specific stimuli. CMV load was measured in parallel.

RESULTS

In individuals progressing to acquired immunodeficiency syndrome with CMV-EOD, CMV-specific IFN- gamma -producing CD4(+) T cells disappeared during the year before onset of CMV-EOD. This disappearance was accompanied by a sharp increase in CMV load before onset of disease. Despite increasing CMV-specific CD8(+) T cell counts, decreasing CMV-specific IFN- gamma -producing CD8(+) T cell counts were found over time. In contrast, the percentage of CMV-specific perforin- and granzyme B-expressing CD8(+) T cells increased.

CONCLUSIONS

Our data indicate that insufficient help of CD4(+) T cells may cause loss of IFN- gamma -producing CD8(+) T cells and loss of control of CMV dissemination. Increasing CMV-infected cell counts in the face of high CMV-specific perforin- and granzyme B-expressing CD8(+) T cell counts may explain the immune pathological characteristics of CMV disease.

Authors+Show Affiliations

Department of Clinical Viro-Immunology, Sanquin Research and Landsteiner Laboratory, Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15717261

Citation

Bronke, Corine, et al. "Dynamics of Cytomegalovirus (CMV)-specific T Cells in HIV-1-infected Individuals Progressing to AIDS With CMV End-organ Disease." The Journal of Infectious Diseases, vol. 191, no. 6, 2005, pp. 873-80.
Bronke C, Palmer NM, Jansen CA, et al. Dynamics of cytomegalovirus (CMV)-specific T cells in HIV-1-infected individuals progressing to AIDS with CMV end-organ disease. J Infect Dis. 2005;191(6):873-80.
Bronke, C., Palmer, N. M., Jansen, C. A., Westerlaken, G. H., Polstra, A. M., Reiss, P., Bakker, M., Miedema, F., Tesselaar, K., & van Baarle, D. (2005). Dynamics of cytomegalovirus (CMV)-specific T cells in HIV-1-infected individuals progressing to AIDS with CMV end-organ disease. The Journal of Infectious Diseases, 191(6), 873-80.
Bronke C, et al. Dynamics of Cytomegalovirus (CMV)-specific T Cells in HIV-1-infected Individuals Progressing to AIDS With CMV End-organ Disease. J Infect Dis. 2005 Mar 15;191(6):873-80. PubMed PMID: 15717261.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamics of cytomegalovirus (CMV)-specific T cells in HIV-1-infected individuals progressing to AIDS with CMV end-organ disease. AU - Bronke,Corine, AU - Palmer,Nanette M, AU - Jansen,Christine A, AU - Westerlaken,Geertje H A, AU - Polstra,Abeltje M, AU - Reiss,Peter, AU - Bakker,Margreet, AU - Miedema,Frank, AU - Tesselaar,Kiki, AU - van Baarle,Debbie, Y1 - 2005/01/31/ PY - 2004/07/13/received PY - 2004/10/04/accepted PY - 2005/2/18/pubmed PY - 2005/4/6/medline PY - 2005/2/18/entrez SP - 873 EP - 80 JF - The Journal of infectious diseases JO - J Infect Dis VL - 191 IS - 6 N2 - BACKGROUND: Since cytomegalovirus (CMV) infection can cause serious clinical complications in immunocompromised individuals, we assessed cellular immune requirements for protection against CMV end-organ disease (CMV-EOD) in human immunodeficiency virus type 1 (HIV-1) infection. METHODS: Longitudinal samples from HIV-1-infected patients in the Amsterdam cohort were analyzed. Dynamics of CMV-specific CD8(+) and CD4(+) T cell responses were analyzed by 4-color fluorescence analysis using major histocompatibility class I CMV peptide-tetramers and by intracellular staining for perforin, granzyme B, and interferon (IFN)- gamma after stimulation with CMV-specific stimuli. CMV load was measured in parallel. RESULTS: In individuals progressing to acquired immunodeficiency syndrome with CMV-EOD, CMV-specific IFN- gamma -producing CD4(+) T cells disappeared during the year before onset of CMV-EOD. This disappearance was accompanied by a sharp increase in CMV load before onset of disease. Despite increasing CMV-specific CD8(+) T cell counts, decreasing CMV-specific IFN- gamma -producing CD8(+) T cell counts were found over time. In contrast, the percentage of CMV-specific perforin- and granzyme B-expressing CD8(+) T cells increased. CONCLUSIONS: Our data indicate that insufficient help of CD4(+) T cells may cause loss of IFN- gamma -producing CD8(+) T cells and loss of control of CMV dissemination. Increasing CMV-infected cell counts in the face of high CMV-specific perforin- and granzyme B-expressing CD8(+) T cell counts may explain the immune pathological characteristics of CMV disease. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/15717261/Dynamics_of_cytomegalovirus__CMV__specific_T_cells_in_HIV_1_infected_individuals_progressing_to_AIDS_with_CMV_end_organ_disease_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1086/427828 DB - PRIME DP - Unbound Medicine ER -